Anti-CD30 抗体 [Ber-H2], prediluted (ab871)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD30 antibody [Ber-H2], prediluted (ab871)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD30 antibody [Ber-H2], prediluted (ab871)

Key features and details

  • Mouse monoclonal [Ber-H2] to CD30, prediluted
  • Suitable for: IHC-P
  • Reacts with: Human
  • Isotype: IgG1

製品の概要

  • 製品名

    Anti-CD30 antibody [Ber-H2], prediluted
    CD30 一次抗体 製品一覧

  • 製品の詳細

    Mouse monoclonal [Ber-H2] to CD30, prediluted

  • 由来種

    Mouse

  • 特異性

    This antibody reacts with mononuclear cells in Hodgkin lymphoma, Reed-Sternberg cells and most anaplastic large cell lymphomas. It stains large cells localized around the B cell in the lymphoid tissues. This antibody stains the cell surface and the golgi region in the cells.

  • アプリケーション

    適用あり: IHC-Pmore details

  • 種交差性

    交差種: Human

  • 免疫原

    BALB/C mice were injected with L428 cell line cells.

  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab871の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IHC-P
Use at an assay dependent concentration.

This antibody has been pretitered and quality controlled to work on formalin-fixed paraffin-embedded as well as acetone fixed cryostat tissue sections. No further titration is required. We suggest an incubation period of 30-60 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Enzymatic predigestion with proteolytic enzymes may increase staining intensity. Although not required, microwave treatment will considerably enhance the signal.
特記事項
IHC-P
Use at an assay dependent concentration.

This antibody has been pretitered and quality controlled to work on formalin-fixed paraffin-embedded as well as acetone fixed cryostat tissue sections. No further titration is required. We suggest an incubation period of 30-60 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Enzymatic predigestion with proteolytic enzymes may increase staining intensity. Although not required, microwave treatment will considerably enhance the signal.

ターゲット情報

  • 機能

    Receptor for TNFSF8/CD30L. May play a role in the regulation of cellular growth and transformation of activated lymphoblasts. Regulates gene expression through activation of NF-kappa-B.

  • 配列類似性

    Contains 6 TNFR-Cys repeats.

  • 翻訳後修飾

    Phosphorylated on serine and tyrosine residues.

  • 細胞内局在

    Cytoplasm and Cell membrane.
  • Information by UniProt

  • 参照データベース

    • 別名

      • CD 30 antibody
      • CD30 antibody
      • CD30 antigen antibody
      • CD30L receptor antibody
      • Cytokine receptor CD30 antibody
      • D1S166E antibody
      • KI 1 antibody
      • KI 1 antigen antibody
      • Ki-1 antigen antibody
      • KI1 antibody
      • Lymphocyte activation antigen CD30 antibody
      • TNFRSF 8 antibody
      • Tnfrsf8 antibody
      • TNR8_HUMAN antibody
      • Tumor necrosis factor receptor superfamily member 8 antibody
      see all

    画像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD30 antibody [Ber-H2], prediluted (ab871)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD30 antibody [Ber-H2], prediluted (ab871)
      Formalin-fixed, paraffin-embeded human Hodgkin's lymphoma tissue stained for CD30 using ab871 in immunohistochemical analysis. Antigen retrieval with EDTA buffer pH 8.0
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD30 antibody [Ber-H2], prediluted (ab871)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD30 antibody [Ber-H2], prediluted (ab871)
      IHC image of ab871 staining in Hodgkin lymphoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab871, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    参考文献 (1)

    ab871 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab871 は 1 報の論文で使用されています。

    • Keren L  et al. MIBI-TOF: A multiplexed imaging platform relates cellular phenotypes and tissue structure. Sci Adv 5:eaax5851 (2019). Mass Cytometry . PubMed: 31633026

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