Anti-CD30 抗体 [Ber-H2], prediluted (ab871)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD30 antibody [Ber-H2], prediluted (ab871)

    Key features and details

    • Mouse monoclonal [Ber-H2] to CD30, prediluted
    • Suitable for: IHC-P
    • Reacts with: Human
    • Isotype: IgG1

    製品の概要

    • 製品名

      Anti-CD30 antibody [Ber-H2], prediluted
      CD30 一次抗体 製品一覧

    • 製品の詳細

      Mouse monoclonal [Ber-H2] to CD30, prediluted

    • 由来種

      Mouse

    • 特異性

      This antibody reacts with mononuclear cells in Hodgkin lymphoma, Reed-Sternberg cells and most anaplastic large cell lymphomas. It stains large cells localized around the B cell in the lymphoid tissues. This antibody stains the cell surface and the golgi region in the cells.

    • アプリケーション

      適用あり: IHC-Pmore details

    • 種交差性

      交差種: Human

    • 免疫原

      BALB/C mice were injected with L428 cell line cells.

    • 特記事項

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    製品の特性

    アプリケーション

    Our Abpromise guarantee covers the use of ab871 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    アプリケーション Abreviews 特記事項
    IHC-P
  • 追加情報
    This antibody has been pretitered and quality controlled to work on formalin-fixed paraffin-embedded as well as acetone fixed cryostat tissue sections.
    No further titration is required.
    We suggest an incubation period of 30-60 minutes at room temperature.
    However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user.
    Enzymatic predigestion with proteolytic enzymes may increase staining intensity.
    Although not required, microwave treatment will considerably enhance the signal.

    • ターゲット情報

    • 機能

      Receptor for TNFSF8/CD30L. May play a role in the regulation of cellular growth and transformation of activated lymphoblasts. Regulates gene expression through activation of NF-kappa-B.

    • 配列類似性

      Contains 6 TNFR-Cys repeats.

    • 翻訳後修飾

      Phosphorylated on serine and tyrosine residues.

    • 細胞内局在

      Cytoplasm and Cell membrane.
    • Information by UniProt

    • 参照データベース

      • 別名

        • CD 30 antibody
        • CD30 antibody
        • CD30 antigen antibody
        • CD30L receptor antibody
        • Cytokine receptor CD30 antibody
        • D1S166E antibody
        • KI 1 antibody
        • KI 1 antigen antibody
        • Ki-1 antigen antibody
        • KI1 antibody
        • Lymphocyte activation antigen CD30 antibody
        • TNFRSF 8 antibody
        • Tnfrsf8 antibody
        • TNR8_HUMAN antibody
        • Tumor necrosis factor receptor superfamily member 8 antibody
        see all

      画像

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD30 antibody [Ber-H2], prediluted (ab871)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD30 antibody [Ber-H2], prediluted (ab871)
        IHC image of ab871 staining in Hodgkin lymphoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab871, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

        For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

      参考文献 (1)

      ab871 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

      ab871 は 1 報の論文で使用されています。

      • Keren L  et al. MIBI-TOF: A multiplexed imaging platform relates cellular phenotypes and tissue structure. Sci Adv 5:eaax5851 (2019). Mass Cytometry . PubMed: 31633026

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