Anti-CD30 抗体 [Ber-H2], prediluted (ab871)
Key features and details
- Mouse monoclonal [Ber-H2] to CD30, prediluted
- Suitable for: IHC-P, IHC-Fr
- Reacts with: Human
- Isotype: IgG1
製品の概要
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製品名
Anti-CD30 antibody [Ber-H2], prediluted
CD30 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [Ber-H2] to CD30, prediluted -
由来種
Mouse -
特異性
This antibody reacts with mononuclear cells in Hodgkin lymphoma, Reed-Sternberg cells and most anaplastic large cell lymphomas. It stains large cells localized around the B cell in the lymphoid tissues. This antibody stains the cell surface and the golgi region in the cells. -
アプリケーション
適用あり: IHC-P, IHC-Frmore details -
種交差性
交差種: Human -
免疫原
BALB/C mice were injected with L428 cell line cells.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
バッファー
Preservative: 0.05% Sodium azide
Inert stabilizer -
Concentration information loading... -
ポリ/モノ
モノクローナル -
クローン名
Ber-H2 -
ミエローマ
unknown -
アイソタイプ
IgG1 -
軽鎖の種類
kappa -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
Our Abpromise guarantee covers the use of ab871 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P | ||
| IHC-Fr |
追加情報
This antibody has been pretitered and quality controlled to work on formalin-fixed paraffin-embedded as well as acetone fixed cryostat tissue sections.
No further titration is required.
We suggest an incubation period of 30-60 minutes at room temperature.
However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user.
Enzymatic predigestion with proteolytic enzymes may increase staining intensity.
Although not required, microwave treatment will considerably enhance the signal.
No further titration is required.
We suggest an incubation period of 30-60 minutes at room temperature.
However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user.
Enzymatic predigestion with proteolytic enzymes may increase staining intensity.
Although not required, microwave treatment will considerably enhance the signal.
ターゲット情報
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機能
Receptor for TNFSF8/CD30L. May play a role in the regulation of cellular growth and transformation of activated lymphoblasts. Regulates gene expression through activation of NF-kappa-B. -
配列類似性
Contains 6 TNFR-Cys repeats. -
翻訳後修飾
Phosphorylated on serine and tyrosine residues. -
細胞内局在
Cytoplasm and Cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 943 Human
- Omim: 153243 Human
- SwissProt: P28908 Human
- Unigene: 1314 Human
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別名
- CD 30 antibody
- CD30 antibody
- CD30 antigen antibody
see all
画像
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD30 antibody [Ber-H2], prediluted (ab871)](https://www.abcam.co.jp/ps/products/0/ab871/Images/ab871-3801-anti-cd30-antibody-ber-h2-prediluted-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD30 antibody [Ber-H2], prediluted (ab871)IHC image of ab871 staining in Hodgkin lymphoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab871, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
データシートおよび資料
参考文献
ab871 は論文での使用が確認できていません。
