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Anti-CD3 zeta 抗体 [BL-336-1B2] - BSA free (ab272071)

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Immunoprecipitation - Anti-CD3 zeta antibody [BL-336-1B2] - BSA free (ab272071)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 zeta antibody [BL-336-1B2] - BSA free (ab272071)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 zeta antibody [BL-336-1B2] - BSA free (ab272071)
  • Anti-CD3 zeta antibody [BL-336-1B2] - BSA free (ab272071)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [BL-336-1B2] to CD3 zeta - BSA free
  • Suitable for: WB, IHC-P, IP
  • Reacts with: Human

製品の概要

  • 製品名

    Anti-CD3 zeta antibody [BL-336-1B2] - BSA free
    CD3 zeta 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [BL-336-1B2] to CD3 zeta - BSA free

  • 由来種

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    IP
    Human
    See all applications and species data

  • 免疫原

    Synthetic peptide within Human CD3 zeta aa 150-164 (C terminal). The exact sequence is proprietary. NP_932170.1 and Gene ID 919.
    Database link: P20963

  • 特記事項

    This product is sold under License from Bethyl Laboratories, Inc.

    ab272071 is the carrier-free version of ab243874. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • バッファー

    pH: 8.20
    Preservative: 0.09% Sodium azide
    Constituent: 98% Borate buffered saline
  • Concentration information loading...

  • 特記事項（精製）

    Recombinant antibody was purified from cell culture supernatant.

  • ポリ/モノ

    モノクローナル

  • クローン名

    BL-336-1B2

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab272071 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

アプリケーション Species
IHC-P
Human
IP
Human
アプリケーション Abreviews 特記事項
WB
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP
Use at an assay dependent concentration.
特記事項
WB
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP
Use at an assay dependent concentration.

ターゲット情報

  • 機能

    Probable role in assembly and expression of the TCR complex as well as signal transduction upon antigen triggering.

  • 関連疾患

    Defects in CD247 are the cause of immunodeficiency due to defect in CD3-zeta (CD3ZID) [MIM:610163]. An immunological deficiency characterized by T-cells impaired immune response to alloantigens, tetanus toxoid and mitogens.

  • 配列類似性

    Belongs to the CD3Z/FCER1G family.
    Contains 3 ITAM domains.

  • ドメイン

    The ITAM domains mediate interaction with SHB.

  • 翻訳後修飾

    Phosphorylated on Tyr residues after T-cell receptor triggering.

  • 細胞内局在

    Membrane.
  • Information by UniProt

  • 参照データベース

    • 別名

      • 4930549J05Rik antibody
      • A430104F18Rik antibody
      • AW552088 antibody
      • CD247 antibody
      • CD247 antigen antibody
      • Cd247 molecule antibody
      • Cd3 antibody
      • CD3 antigen, zeta polypeptide, isoform CRA_b antibody
      • CD3 antigen, zeta subunit antibody
      • CD3-eta antibody
      • CD3H antibody
      • CD3Q antibody
      • Cd3z antibody
      • CD3Z antigen zeta polypeptide (TiT3 complex) antibody
      • CD3Z_HUMAN antibody
      • CD3zeta antibody
      • CD3zeta chain antibody
      • MGC140430 antibody
      • T cell receptor T3 zeta chain antibody
      • T cell surface glycoprotein CD3 zeta chain antibody
      • T-cell antigen receptor complex, zeta subunit of CD3 antibody
      • T-cell receptor T3 zeta chain antibody
      • T-cell surface glycoprotein CD3 zeta chain antibody
      • T3z antibody
      • TCR zeta chain antibody
      • TCRk antibody
      • TCRZ antibody
      • TCRzeta antibody
      see all

    画像

    • Immunoprecipitation - Anti-CD3 zeta antibody [BL-336-1B2] - BSA free (ab272071)
      Immunoprecipitation - Anti-CD3 zeta antibody [BL-336-1B2] - BSA free (ab272071)

      CD3 zeta was immunoprecipitated from 1 mg Jurkat whole cell lysate with ab243874 at 20 µl per reaction Western blot was performed on the immunoprecipitate using ab243874 at 1/1000 dilution.

      Lane 1: ab243874 IP in Jurkat whole cell lysate.

      Lane 2: Contol IgG in Jurkat whole cell lysate.

      Detection: Chemiluminescence with an exposure time of 10 seconds.

      This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (ab243874).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 zeta antibody [BL-336-1B2] - BSA free (ab272071)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 zeta antibody [BL-336-1B2] - BSA free (ab272071)
      Formalin-fixed, paraffin-embedded human metastatic lymph node tissue stained for CD3 zeta using ab243874 at 1/500 dilution in immunohistochemical analysis. A HRP-conjugated goat anti-rabbit IgG was used as the secondary. DAB staining.

      This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (ab243874).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 zeta antibody [BL-336-1B2] - BSA free (ab272071)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 zeta antibody [BL-336-1B2] - BSA free (ab272071)
      Formalin-fixed, paraffin-embedded human tonsil tissue stained for CD3 zeta using ab243874 at 1/500 dilution in immunohistochemical analysis. A HRP-conjugated goat anti-rabbit IgG was used as the secondary. DAB staining.

      This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (ab243874).

    • Anti-CD3 zeta antibody [BL-336-1B2] - BSA free (ab272071)
      Anti-CD3 zeta antibody [BL-336-1B2] - BSA free (ab272071)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab272071 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab272071 は論文での使用が確認できていません。

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