Anti-CD3 epsilon 抗体 [EP449E] (ab52959)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP449E] to CD3 epsilon
- Suitable for: WB, IHC-P, IP
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-CD3 epsilon antibody [EP449E]
CD3 epsilon 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EP449E] to CD3 epsilon -
由来種
Rabbit -
特異性
This antibody reacts with CD3 epsilon. It does not cross-react with other subunits of CD3.
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アプリケーション
適用あり: WB, IHC-P, IPmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide corresponding to Human CD3 epsilon aa 150-250. Derived from the cytoplasmic domain.
Database link: P07766 -
ポジティブ・コントロール
- Jurkat cell lysate and human spleen tissue. IP: Jurkat whole cell lysate.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EP449E -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab52959の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | (1) |
1/1000 - 1/10000. Detects a band of approximately 23 kDa (predicted molecular weight: 23 kDa).
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IHC-P | (2) |
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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IP |
1/20.
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特記事項 |
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WB
1/1000 - 1/10000. Detects a band of approximately 23 kDa (predicted molecular weight: 23 kDa). |
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
IP
1/20. |
ターゲット情報
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機能
The CD3 complex mediates signal transduction. -
配列類似性
Contains 1 Ig-like (immunoglobulin-like) domain.
Contains 1 ITAM domain. -
細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 916 Human
- Omim: 186830 Human
- SwissProt: P07766 Human
- Unigene: 3003 Human
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別名
- CD3 epsilon antibody
- CD3e antibody
- CD3e antigen antibody
see all
画像
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ab52959 at 1/250-1/500 dilution staining CD3 epsilon in human spleen by Immunohistochemistry, Paraffin embedded tissue.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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All lanes : Anti-CD3 epsilon antibody [EP449E] (ab52959) at 1/1000 dilution (purified)
Lane 1 : Jurkat( Human T cell leukemia T lymphocyte )membrane fraction cell lysate
Lane 2 : Jurkat (Human T cell leukemia T lymphocyte) without membrane fraction cell lysate
Lane 3 : Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Predicted band size: 23 kDa
Observed band size: 23 kDa
Exposure time: 20 secondsBlocking/Dilution buffer and concentration: 5% NFDM/TBST
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Purified ab52959 at 1/20 dilution (1µg) immunoprecipitating CD3 epsilon in Jurkat whole cell lysate.
Lane 1 (input): Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate 10µg
Lane 2 (+): ab52959 + Jurkat whole cell lysate.
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab52929 in Jurkat whole cell lysate.
VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
Blocking Buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
Observed band size: 23 kDa -
ab52959 showing positive staining in Normal tonsil tissue.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ab52959 showing positive staining in Normal spleen tissue.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ab52959 showing negative staining in Normal brain tissue.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ab52959 showing negative staining in Normal kidney tissue.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ab52959 showing negative staining in Skeletal muscle tissue.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (15)
ab52959 は 15 報の論文で使用されています。
- Simonson PD et al. Tyramide-conjugated DNA barcodes enable signal amplification for multiparametric CODEX imaging. Commun Biol 5:627 (2022). PubMed: 35754060
- Schaadt NS et al. Graph-based description of tertiary lymphoid organs at single-cell level. PLoS Comput Biol 16:e1007385 (2020). PubMed: 32084130
- Kim D et al. Somatic mTOR mutation in clonally expanded T lymphocytes associated with chronic graft versus host disease. Nat Commun 11:2246 (2020). PubMed: 32382059
- Rao A et al. Cytokines regulate the antigen-presenting characteristics of human circulating and tissue-resident intestinal ILCs. Nat Commun 11:2049 (2020). PubMed: 32341343
- Yang X et al. Misdiagnosis of primary pleural DLBCL as tuberculosis: A case report and literature review. Mol Clin Oncol 8:729-732 (2018). PubMed: 29732155