Anti-CD3 epsilon 抗体 [C3e/3125R] (ab270716)
![Western blot - Anti-CD3 epsilon antibody [C3e/3125R] (ab270716)](https://www.abcam.co.jp/ps/products/270/ab270716/Images/ab270716-381821-Recombinant-Rabbit-Monoclonal-Antibody-C3e-3125r-wb-molt4-cell-lysate.jpg "Western blot - Anti-CD3 epsilon antibody [C3e/3125R] (ab270716)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [C3e/3125R] to CD3 epsilon
- Suitable for: IHC-P, WB
- Reacts with: Human
製品の概要
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製品名
Anti-CD3 epsilon antibody [C3e/3125R]
CD3 epsilon 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [C3e/3125R] to CD3 epsilon -
由来種
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
-
免疫原
Recombinant fragment within Human CD3 epsilon aa 23-119. The exact sequence is proprietary.
Database link: P07766 -
ポジティブ･コントロール
- IHC-P: Human tonsil tissue. WB: MOLT-4 cell lysate.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.05% BSA -
Concentration information loading... -
精製度
Protein A/G purified -
特記事項（精製）
Ab Purified from Bioreactor Concentrate. -
ポリ/モノ
モノクローナル -
クローン名
C3e/3125R -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
アプリケーション
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab270716 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| アプリケーション | Species |
|---|---|
| IHC-P |
Human
|
| WB |
Human
|
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P |
Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
|
| WB |
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 24 kDa.
|
| 特記事項 |
|---|
|
IHC-P
Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
|
WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 24 kDa. |
ターゲット情報
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機能
The CD3 complex mediates signal transduction. -
配列類似性
Contains 1 Ig-like (immunoglobulin-like) domain.
Contains 1 ITAM domain. -
細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 916 Human
- Omim: 186830 Human
- SwissProt: P07766 Human
- Unigene: 3003 Human
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別名
- CD3 epsilon antibody
- CD3e antibody
- CD3e antigen antibody
see all
画像
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Western blot - Anti-CD3 epsilon antibody [C3e/3125R] (ab270716)Anti-CD3 epsilon antibody [C3e/3125R] (ab270716) at 2 µg/ml + MOLT-4 cell lysate.
Predicted band size: 24 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody [C3e/3125R] (ab270716)](https://www.abcam.co.jp/ps/products/270/ab270716/Images/ab270716-381820-Recombinant-Rabbit-Monoclonal-Antibody-C3e-3125r-ihcp-tonsil-tissue-human.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody [C3e/3125R] (ab270716)Formalin-fixed, paraffin-embedded human tonsil tissue stained for CD3 epsilon using ab270716 at 2 μg/ml in immunohistochemical analysis.
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![Anti-CD3 epsilon antibody [C3e/3125R] (ab270716)](https://www.abcam.co.jp/ps/products/270/ab270716/Images/ab270716-3-benefits-of-recombinant-antibodies.png)
Anti-CD3 epsilon antibody [C3e/3125R] (ab270716)
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
参考文献 (0)
ab270716 は論文での使用が確認できていません。
