RabMAb

Anti-CD3 抗体 [SP7], prediluted (ab21703)

Reviews (1)Q&A (2)References (13)

製品の概要

  • 製品名
    Anti-CD3 antibody [SP7], prediluted
    CD3 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [SP7] to CD3, prediluted
  • 由来種
    Rabbit
  • 特異性
    ab21703 recognises CD3 epsilon chain. This antibody reacts with the intracytoplasmic portion of the CD3 antigen expressed by T cells. It stains human T cells in both the cortex and medulla of the thymus and in peripheral lymphoid tissues.
  • アプリケーション
    適用あり: WB, Flow Cyt, IHC-FoFr, IHC-Fr, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Human, Cynomolgus monkey
    交差が予測される動物種: Rat, Sheep, Rabbit, Cow, Dog, Pig, Macaque monkey, Woodchuck
  • 免疫原

    Synthetic peptide corresponding to CD3.

  • ポジティブ･コントロール
    • Recombinant Human CD3 epsilon protein (ab114153) can be used as a positive control in WB. Tonsil tissue.
  • 特記事項


    This antibody is suitable for staining normal and neoplastic T cells.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab21703 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/25. Predicted molecular weight: 19 kDa.
Flow Cyt Use at an assay dependent concentration.
IHC-FoFr Use at an assay dependent concentration.
IHC-Fr 1/1.

Acetone fixed.
IHC-P 1/1. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Use neat. Perform high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0.

ターゲット情報

  • 機能
    The CD3 complex mediates signal transduction.
  • 関連疾患
    Defects in CD3D are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (T(-)/B(+)/NK(+) SCID) [MIM:608971]. A form of severe combined immunodeficiency (SCID), a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. Patients present in infancy recurrent, persistent infections by opportunistic organisms. The common characteristic of all types of SCID is absence of T-cell-mediated cellular immunity due to a defect in T-cell development.
  • 配列類似性
    Contains 1 ITAM domain.
  • 細胞内局在
    Membrane.
  • Information by UniProt
  • 参照データベース
    see all
  • 別名
    • 4930549J05Rik antibody
    • A430104F18Rik antibody
    • AW552088 antibody
    • Cd247 antibody
    • CD247 antigen antibody
    • CD247 antigen, zeta subunit antibody
    • CD247 molecule antibody
    • CD3 antibody
    • CD3 antigen, delta subunit antibody
    • CD3 delta antibody
    • CD3 epsilon antibody
    • CD3 eta antibody
    • CD3 gamma antibody
    • CD3 molecule delta polypeptide antibody
    • CD3 molecule, epsilon polypeptide antibody
    • CD3 molecule, gamma polypeptide antibody
    • CD3 zeta antibody
    • CD3-DELTA antibody
    • CD3d antibody
    • CD3D antigen delta polypeptide antibody
    • CD3d antigen, delta polypeptide (TiT3 complex) antibody
    • CD3d molecule delta antibody
    • CD3d molecule delta CD3 TCR complex antibody
    • CD3d molecule, delta (CD3-TCR complex) antibody
    • CD3D_HUMAN antibody
    • CD3E antibody
    • CD3e antigen antibody
    • CD3E antigen epsilon polypeptide antibody
    • CD3e antigen, epsilon polypeptide (TiT3 complex) antibody
    • CD3e molecule epsilon antibody
    • CD3e molecule epsilon CD3 TCR complex antibody
    • CD3e molecule, epsilon (CD3-TCR complex) antibody
    • CD3epsilon antibody
    • CD3G antibody
    • CD3g antigen antibody
    • CD3G antigen gamma polypeptide antibody
    • CD3g antigen, gamma polypeptide (TiT3 complex) antibody
    • CD3g molecule gamma antibody
    • CD3g molecule gamma CD3 TCR complex antibody
    • CD3g molecule, gamma (CD3-TCR complex) antibody
    • CD3H antibody
    • CD3Q antibody
    • CD3Z antibody
    • CD3zeta antibody
    • Ctg3 antibody
    • FLJ17620 antibody
    • FLJ17664 antibody
    • FLJ18683 antibody
    • FLJ79544 antibody
    • FLJ94613 antibody
    • IMD19 antibody
    • Leu-4 antibody
    • MGC138597 antibody
    • OKT3, delta chain antibody
    • OTTHUMP00000032544 antibody
    • T cell receptor antibody
    • T cell receptor T3 delta chain antibody
    • T cell receptor T3 gamma chain antibody
    • T cell receptor T3 zeta chain antibody
    • T cell receptor zeta chain antibody
    • T cell surface glycoprotein CD3 antibody
    • T cell surface glycoprotein CD3 delta chain antibody
    • T cell surface glycoprotein CD3 epsilon chain antibody
    • T cell surface glycoprotein CD3 gamma chain antibody
    • T cell surface glycoprotein CD3 zeta chain antibody
    • T-cell antigen receptor complex, delta subunit of T3 antibody
    • T-cell antigen receptor complex, epsilon subunit of T3 antibody
    • T-cell antigen receptor complex, gamma subunit of T3 antibody
    • T-cell antigen receptor complex, zeta subunit of CD3 antibody
    • T-cell receptor T3 delta chain antibody
    • T-cell receptor T3 gamma chain antibody
    • T-cell surface antigen T3/Leu-4 epsilon chain antibody
    • T-cell surface glycoprotein CD3 delta chain antibody
    • T-cell surface glycoprotein CD3 gamma chain antibody
    • T3 antibody
    • T3d antibody
    • T3e antibody
    • T3g antibody
    • T3z antibody
    • TCRE antibody
    • TCRk antibody
    • Tcrz antibody
    • TCRzeta antibody
    see all

画像

  • Western blot - Anti-CD3 antibody [SP7], prediluted (ab21703)
    Western blot - Anti-CD3 antibody [SP7], prediluted (ab21703)
    Anti-CD3 antibody [SP7], prediluted (ab21703) at 1/25 dilution + Jurkat cell lysate

    Predicted band size: 19 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 antibody [SP7], prediluted (ab21703)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 antibody [SP7], prediluted (ab21703)
    Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human tonsil tissue, staining CD3 with ab21703.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 antibody [SP7], prediluted (ab21703)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 antibody [SP7], prediluted (ab21703)
    Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human thymus tissue, staining CD3 with ab21703.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 antibody [SP7], prediluted (ab21703)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 antibody [SP7], prediluted (ab21703)
    Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human Hodgkin's lymphoma tissue, staining CD3 with ab21703.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 antibody [SP7], prediluted (ab21703)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 antibody [SP7], prediluted (ab21703)
    Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human uterus tissue, staining CD3 with ab21703.

参考文献

This product has been referenced in:
See all 13 Publications for this product

ab21703 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

レビューと Q&A

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1-3 of 3 Abreviews or Q&A

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD3 antibody [SP7], prediluted

 Excellent
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Breast cancer Eo771)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate Buffer, Vector laboratories(H-3300)
Permeabilization
Yes - 0.2% Triton-X
Specification
Breast cancer Eo771
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C
Fixative
Paraformaldehyde
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Anugraha Rajagopalan

Verified customer

投稿 Mar 01 2018

Question

Hi Kate, Thank you very much. I think we prefer a replacement because a lot of experiments are delayed in our lab because of the non-functional antibody. We prefer to change it to ab828 as I told you before, which we ordered from your company before and it worked very well for our experiments. Thanks again for your help. Have a good day,

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Answer

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement: Order number: #### To check the status of the order please contact our Customer Service team and reference this number. Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know. I wish you the best of luck with your research.  

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Question

Phone enquiry: customer wanted advice in regards to the concentration of the antibody and at what dilution it should be used at for IHC.

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Answer

Thank you for contacting us. As discussed on the phone this antibody is supplied as tissue culture supernatant, the exact concentration of the solution is therefore not know. The product is however prediluted and can therefore be used neat with your IHC staining. I hope this information has been of help. If you require any further information please do not hesitate to contact us again.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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