製品の概要
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製品名Anti-CD3 antibody [SP7], prediluted
CD3 一次抗体 製品一覧 -
製品の詳細Rabbit monoclonal [SP7] to CD3, prediluted
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由来種Rabbit
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特異性ab21703 recognises CD3 epsilon chain. This antibody reacts with the intracytoplasmic portion of the CD3 antigen expressed by T cells. It stains human T cells in both the cortex and medulla of the thymus and in peripheral lymphoid tissues.
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アプリケーション適用あり: WB, IHC-Fr, IHC-Pmore details
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種交差性交差種: Mouse, Human, Cynomolgus monkey
交差が予測される動物種: Rat, Sheep, Rabbit, Cow, Dog, Pig, Macaque monkey, Woodchuck
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免疫原
Synthetic peptide corresponding to CD3.
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ポジティブ･コントロール
- Tonsil tissue.
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特記事項
This antibody is suitable for staining normal and neoplastic T cells.
製品の特性
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製品の状態Prediluted
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保存方法Shipped at 4°C. Store at +4°C.
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バッファーPreservative: 0.05% Sodium Azide
Constituents: Inert stabilizer -
Concentration information loading... -
精製度Tissue culture supernatant
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一次抗体 備考This antibody is suitable for staining normal and neoplastic T cells.
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ポリ/モノモノクローナル
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クローン名SP7
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アイソタイプIgG
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研究分野
関連製品
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Alternative Versions
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Immunohistochemistry kits
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Positive Controls
アプリケーション
Our Abpromise guarantee covers the use of ab21703 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | 1/25. Predicted molecular weight: 19 kDa. | |
| IHC-Fr | 1/1. Acetone fixed. |
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| IHC-P | 1/1. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Use neat. Perform high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0. |
ターゲット情報
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機能The CD3 complex mediates signal transduction.
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関連疾患Defects in CD3D are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (T(-)/B(+)/NK(+) SCID) [MIM:608971]. A form of severe combined immunodeficiency (SCID), a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. Patients present in infancy recurrent, persistent infections by opportunistic organisms. The common characteristic of all types of SCID is absence of T-cell-mediated cellular immunity due to a defect in T-cell development.
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配列類似性Contains 1 ITAM domain.
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細胞内局在Membrane.
- Information by UniProt
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参照データベース
- Entrez Gene: 915 Human
- Entrez Gene: 916 Human
- Entrez Gene: 917 Human
- Entrez Gene: 919 Human
- Entrez Gene: 12500 Mouse
- Entrez Gene: 12501 Mouse
- Entrez Gene: 12502 Mouse
- Entrez Gene: 12503 Mouse
see all -
別名
- 4930549J05Rik antibody
- A430104F18Rik antibody
- AW552088 antibody
see all
画像
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![Western blot - Anti-CD3 antibody [SP7], prediluted (ab21703)](https://www.abcam.co.jp/ps/datasheet/images/21/ab21703/CD3-Primary-antibodies-ab21703-1.jpg)
Western blot - Anti-CD3 antibody [SP7], prediluted (ab21703)Anti-CD3 antibody [SP7], prediluted (ab21703) at 1/25 dilution + Jurkat cell lysate
Predicted band size: 19 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 antibody [SP7], prediluted (ab21703)](https://www.abcam.co.jp/ps/datasheet/images/21/ab21703/CD3-Primary-antibodies-ab21703-2.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 antibody [SP7], prediluted (ab21703)Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human tonsil tissue, staining CD3 with ab21703. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 antibody [SP7], prediluted (ab21703)](https://www.abcam.co.jp/ps/datasheet/images/21/ab21703/CD3-Primary-antibodies-ab21703-3.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 antibody [SP7], prediluted (ab21703)Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human thymus tissue, staining CD3 with ab21703. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 antibody [SP7], prediluted (ab21703)](https://www.abcam.co.jp/ps/datasheet/images/21/ab21703/CD3-Primary-antibodies-ab21703-4.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 antibody [SP7], prediluted (ab21703)Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human Hodgkin's lymphoma tissue, staining CD3 with ab21703. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 antibody [SP7], prediluted (ab21703)](https://www.abcam.co.jp/ps/datasheet/images/21/ab21703/CD3-Primary-antibodies-ab21703-5.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 antibody [SP7], prediluted (ab21703)Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human uterus tissue, staining CD3 with ab21703.
参考文献
This product has been referenced in:
- Wang L et al. An Acquired Vulnerability of Drug-Resistant Melanoma with Therapeutic Potential. Cell 173:1413-1425.e14 (2018). Read more (PubMed: 29754815) »
- Pillon A et al. Actinic keratosis modelling in mice: A translational study. PLoS One 12:e0179991 (2017). Read more (PubMed: 28662116) »
レビューと Q&A
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"