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  1. Link

    cd3-antibody-sp7-prediluted-ab21703.pdf

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Immunology Adaptive Immunity T Cells CD
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RabMAb

Anti-CD3 抗体 [SP7], prediluted (ab21703)

  • Datasheet
  • SDS
Reviews (1)Q&A (2)References (17)

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Western blot - Anti-CD3 antibody [SP7], prediluted (ab21703)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 antibody [SP7], prediluted (ab21703)
  • Flow Cytometry - Anti-CD3 antibody [SP7], prediluted (ab21703)
  • Multiplex immunohistochemistry - Anti-CD3 antibody [SP7], prediluted (ab21703)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 antibody [SP7], prediluted (ab21703)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 antibody [SP7], prediluted (ab21703)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 antibody [SP7], prediluted (ab21703)

Key features and details

  • Rabbit monoclonal [SP7] to CD3, prediluted
  • Suitable for: WB, Flow Cyt, mIHC, IHC-P
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

こちらの製品もご検討ください

一次抗体
Product image
Anti-CD3 antibody [SP162] (ab135372)
二次抗体
Product image
Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
一次抗体
Product image
Alexa Fluor® 488 Anti-CD3 zeta antibody [EP286Y] (ab197532)

関連製品

製品の概要

  • 製品名

    Anti-CD3 antibody [SP7], prediluted
    CD3 一次抗体 製品一覧
  • 製品の詳細

    Rabbit monoclonal [SP7] to CD3, prediluted
  • 由来種

    Rabbit
  • 特異性

    ab21703 recognises CD3 epsilon chain. This antibody reacts with the intracytoplasmic portion of the CD3 antigen expressed by T cells. It stains human T cells in both the cortex and medulla of the thymus and in peripheral lymphoid tissues.
  • アプリケーション

    適用あり: WB, Flow Cyt, mIHC, IHC-Pmore details
  • 種交差性

    交差種: Mouse, Rat, Human
    交差が予測される動物種: Sheep, Rabbit, Cow, Dog, Pig, Cynomolgus monkey, Macaque monkey, Woodchuck
  • 免疫原

    Synthetic peptide within Human CD3 aa 150 to the C-terminus. The exact sequence is proprietary.
    Database link: P07766

  • ポジティブ・コントロール

    • Recombinant Human CD3 epsilon protein (ab114153) can be used as a positive control in WB. Tonsil tissue.
  • 特記事項

    This antibody is suitable for staining normal and neoplastic T cells.

    This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Store at +4°C.
  • バッファー

    pH: 7.60
    Preservative: 0.1% Sodium azide
    Constituents: Tissue culture supernatant, Tris buffered saline, 1% BSA

    Inert stabilizer
  • Concentration information loading...
  • 精製度

    Tissue culture supernatant
  • 一次抗体 備考

    This antibody is suitable for staining normal and neoplastic T cells.
  • ポリ/モノ

    モノクローナル
  • クローン名

    SP7
  • アイソタイプ

    IgG
  • 研究分野

    • Immunology
    • Adaptive Immunity
    • T Cells
    • CD
    • Stem Cells
    • Hematopoietic Progenitors
    • Hematopoietic Stem Cells
    • Human Lineage Negative
    • Immunology
    • Adaptive Immunity
    • Regulatory T Cells

関連製品

  • Alternative Versions

    • Anti-CD3 antibody [SP7] (ab16669)
    • Anti-CD3 antibody [SP7] - BSA and Azide free (ab205228)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • Recombinant Human CD3 epsilon protein (ab114153)

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab21703の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
Use at an assay dependent concentration. Predicted molecular weight: 19 kDa.
Flow Cyt
Use at an assay dependent concentration.
mIHC
Use at an assay dependent concentration.
IHC-P (1)
1/1. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Use neat. Perform high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0.

特記事項
WB
Use at an assay dependent concentration. Predicted molecular weight: 19 kDa.
Flow Cyt
Use at an assay dependent concentration.
mIHC
Use at an assay dependent concentration.
IHC-P
1/1. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Use neat. Perform high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0.

ターゲット情報

  • 機能

    The CD3 complex mediates signal transduction.
  • 関連疾患

    Defects in CD3D are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (T(-)/B(+)/NK(+) SCID) [MIM:608971]. A form of severe combined immunodeficiency (SCID), a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. Patients present in infancy recurrent, persistent infections by opportunistic organisms. The common characteristic of all types of SCID is absence of T-cell-mediated cellular immunity due to a defect in T-cell development.
  • 配列類似性

    Contains 1 ITAM domain.
  • 細胞内局在

    Membrane.
  • Target information above from: UniProt accession P04234 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 参照データベース

    • Entrez Gene: 915 Human
    • Entrez Gene: 916 Human
    • Entrez Gene: 917 Human
    • Entrez Gene: 919 Human
    • Entrez Gene: 12500 Mouse
    • Entrez Gene: 12501 Mouse
    • Entrez Gene: 12502 Mouse
    • Entrez Gene: 12503 Mouse
    • Entrez Gene: 25300 Rat
    • Entrez Gene: 25710 Rat
    • Entrez Gene: 300678 Rat
    • Entrez Gene: 315609 Rat
    • Omim: 186740 Human
    • Omim: 186780 Human
    • Omim: 186790 Human
    • Omim: 186830 Human
    • SwissProt: P04234 Human
    • SwissProt: P07766 Human
    • SwissProt: P09693 Human
    • SwissProt: P20963 Human
    • SwissProt: P04235 Mouse
    • SwissProt: P11942 Mouse
    • SwissProt: P22646 Mouse
    • SwissProt: P24161 Mouse
    • SwissProt: P29020 Mouse
    • SwissProt: P19377 Rat
    • SwissProt: Q64159 Rat
    • Unigene: 504048 Human
    • Unigene: 4527 Mouse
    • Unigene: 10329 Rat
    see all
  • 別名

    • 4930549J05Rik antibody
    • A430104F18Rik antibody
    • AW552088 antibody
    • Cd247 antibody
    • CD247 antigen antibody
    • CD247 antigen, zeta subunit antibody
    • CD247 molecule antibody
    • CD3 antibody
    • CD3 antigen, delta subunit antibody
    • CD3 delta antibody
    • CD3 epsilon antibody
    • CD3 eta antibody
    • CD3 gamma antibody
    • CD3 molecule delta polypeptide antibody
    • CD3 molecule, epsilon polypeptide antibody
    • CD3 molecule, gamma polypeptide antibody
    • CD3 zeta antibody
    • CD3-DELTA antibody
    • CD3d antibody
    • CD3D antigen delta polypeptide antibody
    • CD3d antigen, delta polypeptide (TiT3 complex) antibody
    • CD3d molecule delta antibody
    • CD3d molecule delta CD3 TCR complex antibody
    • CD3d molecule, delta (CD3-TCR complex) antibody
    • CD3D_HUMAN antibody
    • CD3E antibody
    • CD3e antigen antibody
    • CD3E antigen epsilon polypeptide antibody
    • CD3e antigen, epsilon polypeptide (TiT3 complex) antibody
    • CD3e molecule epsilon antibody
    • CD3e molecule epsilon CD3 TCR complex antibody
    • CD3e molecule, epsilon (CD3-TCR complex) antibody
    • CD3epsilon antibody
    • CD3G antibody
    • CD3g antigen antibody
    • CD3G antigen gamma polypeptide antibody
    • CD3g antigen, gamma polypeptide (TiT3 complex) antibody
    • CD3g molecule gamma antibody
    • CD3g molecule gamma CD3 TCR complex antibody
    • CD3g molecule, gamma (CD3-TCR complex) antibody
    • CD3H antibody
    • CD3Q antibody
    • CD3Z antibody
    • CD3zeta antibody
    • Ctg3 antibody
    • FLJ17620 antibody
    • FLJ17664 antibody
    • FLJ18683 antibody
    • FLJ79544 antibody
    • FLJ94613 antibody
    • IMD19 antibody
    • Leu-4 antibody
    • MGC138597 antibody
    • OKT3, delta chain antibody
    • OTTHUMP00000032544 antibody
    • T cell receptor antibody
    • T cell receptor T3 delta chain antibody
    • T cell receptor T3 gamma chain antibody
    • T cell receptor T3 zeta chain antibody
    • T cell receptor zeta chain antibody
    • T cell surface antigen T3/Leu 4 epsilon chain antibody
    • T cell surface glycoprotein CD3 antibody
    • T cell surface glycoprotein CD3 delta chain antibody
    • T cell surface glycoprotein CD3 epsilon chain antibody
    • T cell surface glycoprotein CD3 gamma chain antibody
    • T cell surface glycoprotein CD3 zeta chain antibody
    • T-cell antigen receptor complex, delta subunit of T3 antibody
    • T-cell antigen receptor complex, epsilon subunit of T3 antibody
    • T-cell antigen receptor complex, gamma subunit of T3 antibody
    • T-cell antigen receptor complex, zeta subunit of CD3 antibody
    • T-cell receptor T3 delta chain antibody
    • T-cell receptor T3 gamma chain antibody
    • T-cell surface antigen T3/Leu-4 epsilon chain antibody
    • T-cell surface glycoprotein CD3 delta chain antibody
    • T-cell surface glycoprotein CD3 epsilon chain antibody
    • T-cell surface glycoprotein CD3 gamma chain antibody
    • T3 antibody
    • T3d antibody
    • T3e antibody
    • T3g antibody
    • T3z antibody
    • TCRE antibody
    • TCRk antibody
    • Tcrz antibody
    • TCRzeta antibody
    see all

画像

  • Western blot - Anti-CD3 antibody [SP7], prediluted (ab21703)
    Western blot - Anti-CD3 antibody [SP7], prediluted (ab21703)
    All lanes : Anti-CD3 antibody [SP7] (ab16669) at 1/25 dilution

    Lane 1 : THP1 whole cell lysate (-ve control)
    Lane 2 : Raji whole cell lysate (-ve control)
    Lane 3 : Jurkat whole cell lysate
    Lane 4 : Human Thymus tissue lysate
    Lane 5 : Mouse Thymus tissue lysate
    Lane 6 : Rat Thymus tissue lysate

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

    Predicted band size: 19 kDa
    Observed band size: 23 kDa why is the actual band size different from the predicted?



    Lanes 1 - 6: Merged signal (red and green). Green – ab16669 observed at 23 kDa. Red - loading control, ab8245, observed at 37 kDa.

    This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using Licor blocking buffer before being incubated with ab16669 and ab8245 (loading control) overnight at 4°C. Antibody binding was detected using Goat Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) at a 1:10000 dilution for 1hr at room temperature and then imaged.

    This data was developed using the undiluted version of this antibody (ab16669).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 antibody [SP7], prediluted (ab21703)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 antibody [SP7], prediluted (ab21703)
    Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human tonsil tissue, staining CD3 with ab21703.
  • Flow Cytometry - Anti-CD3 antibody [SP7], prediluted (ab21703)
    Flow Cytometry - Anti-CD3 antibody [SP7], prediluted (ab21703)

    Human peripheral blood lymphocytes stained with ab16669 (red line). Human whole blood was processed using a modified protocol based on Chow et al, 2005 (PMID: 16080188). In brief, human whole blood was fixed in 4% formaldehyde (methanol-free) for 10 min at 22°C. Red blood cells were then lyzed by the addition of Triton X-100 (final concentration - 0.1%) for 15 min at 37°C. For experimentation, cells were treated with 50% methanol (-20°C) for 15 min at 4°C. Cells were then incubated with the antibody (ab16669, 1/1000 dilution) for 30 min at 4°C. The secondary antibody used was Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/2000 dilution for 30 min at 4°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >30,000 total events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. Gating strategy - peripheral blood lymphocytes.

    This data was developed using the undiluted version of this antibody (ab16669).

  • Multiplex immunohistochemistry - Anti-CD3 antibody [SP7], prediluted (ab21703)
    Multiplex immunohistochemistry - Anti-CD3 antibody [SP7], prediluted (ab21703)

    This data was developed using the undiluted version of this antibody (ab16669).

    Fluorescence multiplex immunohistochemical analysis of normal human tonsil tissue (formalin-fixed paraffin-embedded section).

    Merged staining of anti-PD1 (ab237728; orange; Opal™520), anti-PDL1 (ab237726; green; Opal™540), anti-CD68 (ab192847; yellow; Opal™570), anti-CD3 (ab16669; red; Opal™620), anti-Ki67 (ab16667; light blue; Opal™650) and anti-PanCK (ab7753; grey; Opal™690).

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 7-color automation IHC kit (NEL821001KT, Akoya Biosciences®).

    The section was incubated in six rounds of staining; in the order of ab237728 (1/500 dilution), ab237726 (1/500 dilution), ab192847 (1/300 dilution), ab16669 (1/300 dilution), ab16667 (1/200 dilution) and ab7753 (1/200 dilution); each using a separate fluorescent tyramide signal amplification system.

    Sodium citrate antigen retrieval (Leica ER1, pH6.0, 30 minutes) was used in between rounds of tyramide signal amplification to remove the antibody from the previous round, to avoid any cross-reactivity.

    DAPI (dark blue) was used as a nuclear counter stain.

    Microscopy and pseudocoloring of individual Opal™ dyes was performed using a Vectra Polaris.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 antibody [SP7], prediluted (ab21703)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 antibody [SP7], prediluted (ab21703)
    Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human thymus tissue, staining CD3 with ab21703.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 antibody [SP7], prediluted (ab21703)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 antibody [SP7], prediluted (ab21703)
    Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human Hodgkin's lymphoma tissue, staining CD3 with ab21703.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 antibody [SP7], prediluted (ab21703)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 antibody [SP7], prediluted (ab21703)
    Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human uterus tissue, staining CD3 with ab21703.

プロトコール

  • Immunohistochemistry protocols

Click here to view the general protocols

データシートおよび資料

  • SDS download

  • Datasheet download

    Download

参考文献 (17)

ab21703 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab21703 は 17 報の論文で使用されています。

  • Schlesinger Y  et al. Single-cell transcriptomes of pancreatic preinvasive lesions and cancer reveal acinar metaplastic cells' heterogeneity. Nat Commun 11:4516 (2020). PubMed: 32908137
  • Kisu I  et al. Long-Term Outcome and Rejection After Allogeneic Uterus Transplantation in Cynomolgus Macaques. J Clin Med 8:N/A (2019). PubMed: 31581534
  • Schropp V  et al. Contribution of LTi and TH17 cells to B cell aggregate formation in the central nervous system in a mouse model of multiple sclerosis. J Neuroinflammation 16:111 (2019). PubMed: 31138214
  • Buss LA & Dachs GU Voluntary exercise slows breast tumor establishment and reduces tumor hypoxia in ApoE-/- mice. J Appl Physiol (1985) 124:938-949 (2018). PubMed: 29357514
  • Wang L  et al. An Acquired Vulnerability of Drug-Resistant Melanoma with Therapeutic Potential. Cell 173:1413-1425.e14 (2018). PubMed: 29754815
View all Publications for this product

レビューと Q&A

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レビューを送る 質問を送る

1-3 of 3 Abreviews or Q&A

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD3 antibody [SP7], prediluted

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Breast cancer Eo771)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate Buffer, Vector laboratories(H-3300)
Permeabilization
Yes - 0.2% Triton-X
Specification
Breast cancer Eo771
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C
Fixative
Paraformaldehyde
Read More

Anugraha Rajagopalan

Verified customer

投稿 Mar 01 2018

Question

Hi Kate, Thank you very much. I think we prefer a replacement because a lot of experiments are delayed in our lab because of the non-functional antibody. We prefer to change it to ab828 as I told you before, which we ordered from your company before and it worked very well for our experiments. Thanks again for your help. Have a good day,

Read More

Abcam community

Verified customer

Asked on Dec 19 2011

Answer

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement: Order number: #### To check the status of the order please contact our Customer Service team and reference this number. Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know. I wish you the best of luck with your research.  

Read More

Abcam Scientific Support

Answered on Dec 19 2011

Question

Phone enquiry: customer wanted advice in regards to the concentration of the antibody and at what dilution it should be used at for IHC.

Read More

Abcam community

Verified customer

Asked on Nov 24 2011

Answer

Thank you for contacting us. As discussed on the phone this antibody is supplied as tissue culture supernatant, the exact concentration of the solution is therefore not know. The product is however prediluted and can therefore be used neat with your IHC staining. I hope this information has been of help. If you require any further information please do not hesitate to contact us again.

Read More

Abcam Scientific Support

Answered on Nov 24 2011

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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