Anti-CD3 抗体 [PS1] (ab699)
製品の概要
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製品名
Anti-CD3 antibody [PS1]
CD3 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [PS1] to CD3 -
由来種
Mouse -
特異性
This antibody, when used in conjunction with UCHL-1, is regarded as a reliable pan T-cell marker for immunophenotyping of lymphomas (See reference). Exceptions include some of the more aggressive, high-grade T cell lymphomas, which may not express detectable antigen. This antibody does not react with B-cells. -
アプリケーション
適用あり: IHC-P, IHC-Fr, WB, ICC/IFmore details -
種交差性
交差種: Human -
免疫原
Prokaryotic fusion protein corresponding to the epsilon chain of CD3
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ポジティブ･コントロール
- Tonsil or T-cell lymphoma. IHC-P: Human colon tissue and colorectal mucosa tissue. IHC-Fr: Human skin tissue.
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特記事項
This antibody works well when used in combination with ab972 - Antigen retrieval solution - heat mediated high pH 9.5.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: Proprietary, not sodium azide or thimerosal.
Constituents: PBS, Tissue Culture Supernatant. pH 7.3, Protein carrier. -
Concentration information loading... -
ポリ/モノ
モノクローナル -
クローン名
PS1 -
ミエローマ
unknown -
アイソタイプ
IgG2a -
軽鎖の種類
unknown -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
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Unmasking reagent
アプリケーション
Our Abpromise guarantee covers the use of ab699 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P | Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. | |
| IHC-Fr | Use at an assay dependent concentration. | |
| WB | Use at an assay dependent concentration. PubMed: 21654633 | |
| ICC/IF | Use at an assay dependent concentration. |
ターゲット情報
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機能
The CD3 complex mediates signal transduction. -
関連疾患
Defects in CD3D are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (T(-)/B(+)/NK(+) SCID) [MIM:608971]. A form of severe combined immunodeficiency (SCID), a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. Patients present in infancy recurrent, persistent infections by opportunistic organisms. The common characteristic of all types of SCID is absence of T-cell-mediated cellular immunity due to a defect in T-cell development. -
配列類似性
Contains 1 ITAM domain. -
細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 915 Human
- Entrez Gene: 916 Human
- Entrez Gene: 917 Human
- Entrez Gene: 919 Human
- Omim: 186740 Human
- Omim: 186780 Human
- Omim: 186790 Human
- Omim: 186830 Human
see all -
別名
- 4930549J05Rik antibody
- A430104F18Rik antibody
- AW552088 antibody
see all
画像
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 antibody [PS1] (ab699)](https://www.abcam.co.jp/ps/products/0/ab699/Images/ab699-3649-anti-cd3-antibody-ps1-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 antibody [PS1] (ab699)This image is courtesy of an anonymous Abreviewab699 staining CD3 in paraffin-embedded human colorectal mucosa tissue sections by IHC-P. Tissue was fixed with formaldehyde and blocked with 10% Goat serum for 30 minutes at 20°C; antigen retrieval was by heat mediation in citrate buffer (pH 6). Samples were incubated with primary antibody (1/50 in PBS/BSA/Tween) for 30 minutes at 20°C. An undiluted HRP-conjugated rat anti mouse/rabbit polymer was used as secondary antibody.
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![Immunohistochemistry (Frozen sections) - Anti-CD3 antibody [PS1] (ab699)](https://www.abcam.co.jp/ps/products/0/ab699/Images/ab699-3648-anti-cd3-antibody-ps1-immunohistochemistry.jpg)
Immunohistochemistry (Frozen sections) - Anti-CD3 antibody [PS1] (ab699)Image courtesy of an anonymous Abreview.ab699 staining CD3 in human colon tissue by immunohistochemistry. Tissue was fixed in acetone, blocked using 10% donkey serum/PBS for 30 minutes at 21°C and then incubated with ab699 at a 1/40 dilution for 16 hours at 4°C. The secondary used was an Alexa-Fluor® 555 conjugated donkey anti-mouse polyclonal used at a 1/1000 dilution. CD3 in red nuclear counterstaining with DAPI in blue.
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![Western blot - Anti-CD3 antibody [PS1] (ab699)](https://www.abcam.co.jp/ps/products/0/ab699/Images/ab699-282859-anti-cd3-antibody-ps1-western-blot.jpg)
Western blot - Anti-CD3 antibody [PS1] (ab699)All lanes : Anti-CD3 antibody [PS1] (ab699) at 1/1000 dilution
Lane 1 : Positive control - CD3 protein
Lane 2 : Whole cell Jurkat lysate
Lane 3 : Whole cell PBMCs lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat polyclonal anti-mouse CD3 HRP at 1/2000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Exposure time: 10 minutesGel running conditions: Reduced non-denaturing (native) (5-16% gels).
Incubation time: 16 hours at 4°C in 5% BSA/ TBST.
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![Immunocytochemistry/ Immunofluorescence - Anti-CD3 antibody [PS1] (ab699)](https://www.abcam.co.jp/ps/products/0/ab699/Images/ab699-282860-anti-cd3-antibody-ps1-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-CD3 antibody [PS1] (ab699)Methanol fixed human lymphocyte cells stained for CD3 (red) using ab699 at a 1/75 dilution in ICC/IF. Blocked for 30 minutes at RT using 4% BSA. Incubation time: 16 hours at 4°C in PBS/4% BSA. Secondary antibody: Goat polyclonal anti-mouse TexasRed® at a 1/100 dilution.
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![Immunohistochemistry (Frozen sections) - Anti-CD3 antibody [PS1] (ab699)](https://www.abcam.co.jp/ps/products/0/ab699/Images/ab699-3647-anti-cd3-antibody-ps1-immunohistochemistry.jpg)
Immunohistochemistry (Frozen sections) - Anti-CD3 antibody [PS1] (ab699)Image courtesy of an anonymous Abreview.ab669 staining CD3 in human skin tissue by Immunohistochemistry (Frozen sections). Tissue was fixed in methanol, blocked using 4% BSA for 30 minutes at room temperature and then incubated with ab699 at a 1/75 dilution for 16 hours at 4°C. The secondary used was a biotin conjugated goat anti-mouse polyclonal used at 10µg/ml.
プロトコール
参考文献
This product has been referenced in:
- Matsuda M et al. Human NK cell development in hIL-7 and hIL-15 knockin NOD/SCID/IL2rgKO mice. Life Sci Alliance 2:N/A (2019). Read more (PubMed: 30936185) »
- Peruzzotti-Jametti L et al. Macrophage-Derived Extracellular Succinate Licenses Neural Stem Cells to Suppress Chronic Neuroinflammation. Cell Stem Cell 22:355-368.e13 (2018). Read more (PubMed: 29478844) »
