Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Colon)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate pH 6 & TRIS pH 9
Permeabilization
Yes - 0.3% Triton X-100
Specification
Colon
Blocking step
10% Donkey Serum + 3% BSA as blocking agent for 24 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C
Fixative
Paraformaldehyde
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 20°C · Diluent: 3% BSA in 1xPBS
Dilution
1/20
Secondary antibody
Secondary antibody
None used
Additional data
Additional Notes
Staining appears to be weak when using GE Cell DIVE.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Dylan Windell
Verified customer
投稿 Oct 01 2021