Anti-CD3 抗体 (ab5690)
Key features and details
- Rabbit polyclonal to CD3
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-CD3 antibody
CD3 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to CD3 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide:
KAKAKPVTRGAGA
, corresponding to amino acids 156-168 of Human CD3 Epsilon chain. -
特記事項
Abcam is committed to meeting high standards of manufacturing and has decided to discontinue this product once the stock runs out as we are unable to secure its future high-quality supply. We suggest ab16669 as possible replacement. We are sorry for any inconvenience this may case.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
バッファー
pH: 7.40
Preservative: 0.05% Sodium azide
Constituent: PBS -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
アプリケーション
Our Abpromise guarantee covers the use of ab5690 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
アプリケーション | Abreviews | 特記事項 |
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WB | Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 23 kDa. | |
IHC-P | 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. The recommended starting incubation time is 10min. |
ターゲット情報
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機能
The CD3 complex mediates signal transduction. -
関連疾患
Defects in CD3D are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (T(-)/B(+)/NK(+) SCID) [MIM:608971]. A form of severe combined immunodeficiency (SCID), a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. Patients present in infancy recurrent, persistent infections by opportunistic organisms. The common characteristic of all types of SCID is absence of T-cell-mediated cellular immunity due to a defect in T-cell development. -
配列類似性
Contains 1 ITAM domain. -
細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 915 Human
- Entrez Gene: 916 Human
- Entrez Gene: 917 Human
- Entrez Gene: 919 Human
- Entrez Gene: 12500 Mouse
- Entrez Gene: 12501 Mouse
- Entrez Gene: 12502 Mouse
- Entrez Gene: 12503 Mouse
see all -
別名
- 4930549J05Rik antibody
- A430104F18Rik antibody
- AW552088 antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 antibody (ab5690)Viswanathan, K. et al PLoS One. 2012;7(9):e44694. doi: 10.1371/journal.pone.0044694. Epub 2012 Sep 26 Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/
CD3 and active Caspase 3 populations 72 hrs after mouse aortic allograft
C57Bl/6 donor aortic allografts were transplanted into Balb/C recipient mice (N = 3 per treatment) and followed up at 72hrs. Compared to saline, Serp-2 but not CrmA treatment reduced caspase 3 activity (panels A-C; p<0.0224). Neither protein treatment significantly reduced CD3+ T cells (panels D-F).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 antibody (ab5690)Divan, A. et al LoS One. 2018 May 3;13(5):e0196893. doi: 10.1371/journal.pone.0196893. eCollection 2018 Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/
T cells in central nervous system during late disseminated infection
A-E) Representative epifluorescence images of T cells (CD3), blood vessels (CD31), and nucleated cells (DAPI) in the brain, dura mater, and pia mater. (A) Epifluorescence images described from left to right. CD3 shown in FITC channel; CD31+ blood vessels shown in TRITC channel; nucleated cells shown in DAPI channel; merged image showing CD3+ cell associated with pia mater within the commissure of the isocortex. (B) T cells within the lymphatic-like vascular region of the sagittal sinus in the dura mater. (C) T cell associated with a blood vessel in the vasculature of the brain choroid plexus. (D) T cell associated with blood vessel in the dura mater. (E) T cell in extravascular region of the dura mater.
(After Figure 3 of Dirvan et al)
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All lanes : Anti-CD3 antibody (ab5690) at 1 µg/ml
Lane 1 : THP1 whole cell lysate (-ve control)
Lane 2 : Raji whole cell lysate (-ve control)
Lane 3 : Jurkat whole cell lysate
Lane 4 : Human Thymus tissue lysate
Lane 5 : Mouse Thymus tissue lysate
Lane 6 : Rat Thymus tissue lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 23 kDa
Observed band size: 23 kDaLanes 1 - 6: Merged signal (red and green). Green – ab5690 observed at 23 kDa. Red - loading control, ab8245, observed at 37 kDa.
This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using Licor blocking buffer before being incubated with ab5690 and ab8245 (loading control) overnight at 4°C. Antibody binding was detected using Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) at a 1:10000 dilution for 1hr at room temperature and then imaged.
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IHC image of CD3 staining in a formalin fixed, paraffin embedded normal human tonsil tissue section*, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 20 mins. The section was then incubated with ab5690 at 1/100 dilution for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
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Lanes 1-2 : Anti-CD3 antibody (ab5690) at 1 µg/ml
Lanes 3-4 : No primary antibody
Lanes 1 & 3 : Jurkat cell lysate at 30 µg
Lanes 2 & 4 : Rat thymus tissue lysate at 20 µg
Secondary
All lanes : Goat anti-rabbit IgG (H+L), highly cross - adsorbed, HiLyte™ Fluor 750-labeled at 1/12500 dilution
Predicted band size: 23 kDa
Observed band size: 23 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human spleen tissue labelling CD3 with ab5690 at 2µl/ml. Slides were steamed in IHC epitope retrieval solution for 35 minutes and then cooled for 20 minutes. Samples were incubated with the primary antibody at room temperature for 1 hour, incubated with a biotinylated secondary antibody for 30 minutes followed by HRP-Streptavidin for 30 minutes. Developed with DAB chromogen substrate for 5-10 minutes. Counter stained with hematoxylin. Magnification: 10X.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human spleen tissue labelling CD3 with ab5690 at 2µl/ml. Slides were steamed in IHC epitope retrieval solution for 35 minutes and then cooled for 20 minutes. Samples were incubated with the primary antibody at room temperature for 1 hour, incubated with a biotinylated secondary antibody for 30 minutes followed by HRP-Streptavidin for 30 minutes. Developed with DAB chromogen substrate for 5-10 minutes. Counter stained with hematoxylin. Magnification: 40X.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse spleen tissue labelling CD3 with ab5690 at 2µl/ml. Slides were steamed in IHC epitope retrieval solution for 35 minutes and then cooled for 20 minutes. Samples were incubated with the primary antibody at room temperature for 1 hour, incubated with a biotinylated secondary antibody for 30 minutes followed by HRP-Streptavidin for 30 minutes. Developed with DAB chromogen substrate for 5-10 minutes. Counter stained with hematoxylin. Magnification: 10X.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse spleen tissue labelling CD3 with ab5690 at 2µl/ml. Slides were steamed in IHC epitope retrieval solution for 35 minutes and then cooled for 20 minutes. Samples were incubated with the primary antibody at room temperature for 1 hour, incubated with a biotinylated secondary antibody for 30 minutes followed by HRP-Streptavidin for 30 minutes. Developed with DAB chromogen substrate for 5-10 minutes. Counter stained with hematoxylin. Magnification: 40X.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human skeletal muscle tissue showing no expression of CD3 when labelled with ab5690 at 2µl/ml. Slides were steamed in IHC epitope retrieval solution for 35 minutes and then cooled for 20 minutes. Samples were incubated with the primary antibody at room temperature for 1 hour, incubated with a biotinylated secondary antibody for 30 minutes followed by HRP-Streptavidin for 30 minutes. Developed with DAB chromogen substrate for 5-10 minutes. Counter stained with hematoxylin. Magnification: 40X.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 antibody (ab5690)This image is courtesy of an Anonymous Abreview.ab5690 staining CD3 in human lymph node tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded tissue sections). The sections were fixed in paraformaldehyde and subjected to heat-mediated antigen retrieval in citric buffer pH 6.0, prior to blocking with 10% serum for 1 hour at 20°C. The primary antibody was diluted 1/100 and incubated with the sample for 12 hours at 4°C. An HRP-conjugated goat anti-rabbit polyclonal was used as the secondary antibody, diluted 1/200.
プロトコール
参考文献 (323)
ab5690 は 323 報の論文で使用されています。
- Kim KW et al. FUT1 deficiency elicits immune dysregulation and corneal opacity in steady state and under stress. Cell Death Dis 11:285 (2020). PubMed: 32332708
- Yin P et al. Intranasal Delivery of Immunotherapeutic Nanoformulations for Treatment of Glioma Through in situ Activation of Immune Response. Int J Nanomedicine 15:1499-1515 (2020). PubMed: 32189965
- Cora MC et al. Previously Diagnosed Reticulum Cell Hyperplasia in Decalcified Rat Bone Marrow Stain Positive for Ionized Calcium Binding Adapter Molecule 1 (Iba1): A Monocytic/Macrophage Cell Marker. Toxicol Pathol 48:317-322 (2020). PubMed: 31801420
- Gruber ES et al. The Determination of Immunomodulation and Its Impact on Survival of Rectal Cancer Patients Depends on the Area Comprising a Tissue Microarray. Cancers (Basel) 12:N/A (2020). PubMed: 32121328
- Gilley KN et al. Influence of total western diet on docosahexaenoic acid suppression of silica-triggered lupus flaring in NZBWF1 mice. PLoS One 15:e0233183 (2020). PubMed: 32413078
レビューと Q&A
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