Anti-CD28 抗体 [EPR23280-63] - BSA and Azide free (ab270034)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23280-63] to CD28 - BSA and Azide free
- Suitable for: Flow Cyt
- Reacts with: Mouse
Related conjugates and formulations
製品の概要
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製品名
Anti-CD28 antibody [EPR23280-63] - BSA and Azide free
CD28 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR23280-63] to CD28 - BSA and Azide free -
由来種
Rabbit -
アプリケーション
適用あり: Flow Cytmore details
適用なし: ICC/IF,IHC-P,IP or WB -
種交差性
交差種: Mouse -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- Flow cyt: Mouse splenocytes.
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特記事項
ab270034 is the carrier-free version of ab246517.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
バッファー
pH: 7.2
Constituent: PBS -
キャリア・フリー
はい -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR23280-63 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab270034の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
Flow Cyt |
Use at an assay dependent concentration.
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特記事項 |
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Flow Cyt
Use at an assay dependent concentration. |
ターゲット情報
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機能
Involved in T-cell activation, the induction of cell proliferation and cytokine production and promotion of T-cell survival. -
組織特異性
Expressed in T-cells and plasma cells, but not in less mature B-cells. -
配列類似性
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 12487 Mouse
- SwissProt: P31041 Mouse
- Unigene: 255003 Mouse
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別名
- CD 28 antibody
- CD28 antibody
- CD28 antigen antibody
see all
画像
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Flow cytometric analysis of mouse splenocytes labelling CD28 with ab246517 at 1/500 dilution (0.1ug) (Right) compared with a Rabbit monoclonal IgG isotype control (ab172730) (Left). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.
Cells were stained with rabbit IgG (Left) or ab246517 (Right). Then stained with anti-CD3 conjugated to Alexa Fluor® 647.
Gated on viable cells.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab246517).
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
Certificate of Compliance
参考文献 (0)
ab270034 は論文での使用が確認できていません。