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Anti-CD276 抗体 [SP265] - C-terminal (ab227679)

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Immunocytochemistry - Anti-CD276 antibody [SP265] - C-terminal (ab227679)
  • Western blot - Anti-CD276 antibody [SP265] - C-terminal (ab227679)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD276 antibody [SP265] - C-terminal (ab227679)
  • Immunocytochemistry/ Immunofluorescence - Anti-CD276 antibody [SP265] - C-terminal (ab227679)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD276 antibody [SP265] - C-terminal (ab227679)
  • Flow Cytometry - Anti-CD276 antibody [SP265] - C-terminal (ab227679)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD276 antibody [SP265] - C-terminal (ab227679)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD276 antibody [SP265] - C-terminal (ab227679)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD276 antibody [SP265] - C-terminal (ab227679)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD276 antibody [SP265] - C-terminal (ab227679)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD276 antibody [SP265] - C-terminal (ab227679)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD276 antibody [SP265] - C-terminal (ab227679)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD276 antibody [SP265] - C-terminal (ab227679)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD276 antibody [SP265] - C-terminal (ab227679)
  • Anti-CD276 antibody [SP265] - C-terminal (ab227679)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [SP265] to CD276 - C-terminal
  • Suitable for: ICC/IF, IHC-P, WB, Flow Cyt
  • Knockout validated
  • Reacts with: Human

製品の概要

  • 製品名

    Anti-CD276 antibody [SP265] - C-terminal
    CD276 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [SP265] to CD276 - C-terminal

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: ICC/IF, IHC-P, WB, Flow Cytmore details

  • 種交差性

    交差種: Human
    交差が予測される動物種: Mouse, Rat

  • 免疫原

    Synthetic peptide within Human CD276 aa 500 to the C-terminus (C terminal). The exact sequence is proprietary.
    Database link: Q5ZPR3

  • ポジティブ･コントロール

    • WB: Hs 700T cell lysate. IHC-P: Human ovarian adenocarcinoma, lung squamous cell carcinoma, renal cell carcinoma, liver hepatoma, bladder transitional cell carcinoma, cervical squamous cell carcinoma, skin squamous cell carcinoma, skin melanoma, Hodgkin's lymphoma and prostate adenocarcinoma tissue. Flow cytometry: PC-3 cells. ICC: HEK293 and LNCaP cells

  • 特記事項

    This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab227679 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF 1/25 - 1/100.
IHC-P 1/100.

For antigen retrieval: Boil tissue section in EDTA buffer, pH 8.0 for 10 minutes followed by cooling at room temperature for 20 minutes.
WB 1/100. Predicted molecular weight: 57 kDa.
Flow Cyt 1/400.

ターゲット情報

  • 機能

    May participate in the regulation of T-cell-mediated immune response. May play a protective role in tumor cells by inhibiting natural-killer mediated cell lysis as well as a role of marker for detection of neuroblastoma cells. May be involved in the development of acute and chronic transplant rejection and in the regulation of lymphocytic activity at mucosal surfaces. Could also play a key role in providing the placenta and fetus with a suitable immunological environment throughout pregnancy. Both isoform 1 and isoform 2 appear to be redundant in their ability to modulate CD4 T-cell responses. Isoform 2 is shown to enhance the induction of cytotoxic T-cells and selectively stimulates interferon gamma production in the presence of T-cell receptor signaling.

  • 組織特異性

    Ubiquitous but not detectable in peripheral blood lymphocytes or granulocytes. Weakly expressed in resting monocytes. Expressed in dendritic cells derived from monocytes. Expressed in epithelial cells of sinonasal tissue. Expressed in extravillous trophoblast cells and Hofbauer cells of the first trimester placenta and term placenta.

  • 配列類似性

    Belongs to the immunoglobulin superfamily. BTN/MOG family.
    Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 2 Ig-like V-type (immunoglobulin-like) domains.

  • 細胞内局在

    Membrane.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • 4Ig B7 H3 antibody
    • 4Ig-B7-H3 antibody
    • AU016588 antibody
    • B7 H3 antibody
    • B7 homolog 3 antibody
    • B7-H3 antibody
    • B7H3 antibody
    • B7RP-2 antibody
    • CD_antigen=CD276 antibody
    • CD276 antibody
    • CD276 antigen antibody
    • CD276 molecule antibody
    • CD276_HUMAN antibody
    • Costimulatory molecule antibody
    • Flags: Precursor antibody
    • PSEC0249 antibody
    • UNQ309/PRO352 antibody
    see all

画像

  • Immunocytochemistry - Anti-CD276 antibody [SP265] - C-terminal (ab227679)
    Immunocytochemistry - Anti-CD276 antibody [SP265] - C-terminal (ab227679)
    ab227679 staining CD276 in wild-type HEK293 cells (top panel) and CD276 knockout HEK293 cells (ab266658) (bottom panel). The cells were fixed with 100% methanol (5 min) then permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab227679 at 1/100 dilution and ab7291 (Mouse monoclonal to alpha Tubulin) at 1/1000 dilution overnight at 4°C followed by a further incubation at room temperature for 1h with a goat secondary antibody to rabbit IgG (Alexa Fluor® 488) (ab150081) at 2 μg/ml (shown in green) and a goat secondary antibody to mouse IgG (Alexa Fluor® 594) (ab150120) at 2 μg/ml (shown in red). Nuclear DNA was labelled in blue with DAPI.
    Image was taken with a confocal microscope (Leica-Microsystems TCS SP8).
  • Western blot - Anti-CD276 antibody [SP265] - C-terminal (ab227679)
    Western blot - Anti-CD276 antibody [SP265] - C-terminal (ab227679)
    Anti-CD276 antibody [SP265] - C-terminal (ab227679) at 1/100 dilution + Hs 700T (human pancreatic adenocarcinoma cell line) cell lysate

    Developed using the ECL technique.

    Predicted band size: 57 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD276 antibody [SP265] - C-terminal (ab227679)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD276 antibody [SP265] - C-terminal (ab227679)

    Formalin-fixed, paraffin-embedded human ovarian adenocarcinoma tissue stained for CD276 with ab227679 at 1/100 dilution in immunohistochemical analysis.

  • Immunocytochemistry/ Immunofluorescence - Anti-CD276 antibody [SP265] - C-terminal (ab227679)
    Immunocytochemistry/ Immunofluorescence - Anti-CD276 antibody [SP265] - C-terminal (ab227679)

    Immunocytochemistry/ Immunofluorescence analysis of LNCaP (Human prostate carcinoma epithelial cell) cells labeling CD276  with purified ab227679 at 1/25 (9.24 µg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD276 antibody [SP265] - C-terminal (ab227679)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD276 antibody [SP265] - C-terminal (ab227679)

    Formalin-fixed, paraffin-embedded human bladder transitional cell carcinoma tissue stained for CD276 with ab227679 at 1/100 dilution in immunohistochemical analysis.

  • Flow Cytometry - Anti-CD276 antibody [SP265] - C-terminal (ab227679)
    Flow Cytometry - Anti-CD276 antibody [SP265] - C-terminal (ab227679)

    Flow cytometric analysis of PC-3 (human prostate adenocarcinoma cell line) cell line labeling CC276 with ab227679 at 1/400 (green) compared with a rabbit isotype control (blue).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD276 antibody [SP265] - C-terminal (ab227679)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD276 antibody [SP265] - C-terminal (ab227679)

    Formalin-fixed, paraffin-embedded human lung squamous cell carcinoma tissue stained for CD276 with ab227679 at 1/100 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD276 antibody [SP265] - C-terminal (ab227679)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD276 antibody [SP265] - C-terminal (ab227679)

    Formalin-fixed, paraffin-embedded human renal cell carcinoma tissue stained for CD276 with ab227679 at 1/100 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD276 antibody [SP265] - C-terminal (ab227679)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD276 antibody [SP265] - C-terminal (ab227679)

    Formalin-fixed, paraffin-embedded human liver hepatoma tissue stained for CD276 with ab227679 at 1/100 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD276 antibody [SP265] - C-terminal (ab227679)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD276 antibody [SP265] - C-terminal (ab227679)

    Formalin-fixed, paraffin-embedded human cervical squamous cell carcinoma tissue stained for CD276 with ab227679 at 1/100 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD276 antibody [SP265] - C-terminal (ab227679)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD276 antibody [SP265] - C-terminal (ab227679)

    Formalin-fixed, paraffin-embedded human skin squamous cell carcinoma tissue stained for CD276 with ab227679 at 1/100 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD276 antibody [SP265] - C-terminal (ab227679)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD276 antibody [SP265] - C-terminal (ab227679)

    Formalin-fixed, paraffin-embedded human skin melanoma tissue stained for CD276 with ab227679 at 1/100 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD276 antibody [SP265] - C-terminal (ab227679)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD276 antibody [SP265] - C-terminal (ab227679)

    Formalin-fixed, paraffin-embedded human Hodgkin's lymphoma tissue stained for CD276 with ab227679 at 1/100 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD276 antibody [SP265] - C-terminal (ab227679)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD276 antibody [SP265] - C-terminal (ab227679)

    Formalin-fixed, paraffin-embedded human prostate adenocarcinoma tissue stained for CD276 with ab227679 at 1/100 dilution in immunohistochemical analysis.

  • Anti-CD276 antibody [SP265] - C-terminal (ab227679)
    Anti-CD276 antibody [SP265] - C-terminal (ab227679)

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

参考文献 (1)

ab227679 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab227679 は 1 報の論文で使用されています。

  • Li H  et al. MEK Inhibitor Augments Antitumor Activity of B7-H3-Redirected Bispecific Antibody. Front Oncol 10:1527 (2020). PubMed: 32984002

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