Anti-CD276 抗体 [6A1] (ab105922)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD276 antibody [6A1] (ab105922)](https://www.abcam.co.jp/ps/products/105/ab105922/Images/ab105922-118300-anti-cd276-antibody-6a1-immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD276 antibody [6A1] (ab105922)")
Key features and details
- Mouse monoclonal [6A1] to CD276
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG1
製品の概要
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製品名
Anti-CD276 antibody [6A1]
CD276 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [6A1] to CD276 -
由来種
Mouse -
アプリケーション
適用あり: WB, IHC-Pmore details
適用なし: Flow Cyt -
種交差性
交差種: Human -
免疫原
Recombinant fragment corresponding to Human CD276 aa 30-130.
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ポジティブ･コントロール
- WB: HEK293 cell lysate IHC-P: Human cervical cancer and ovarian cancer tissues.
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特記事項
This product was changed from ascites to supernatant. Lot no’s high than GR209552-21 are from Tissue Culture Supernatant
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. -
バッファー
Preservative: 0.05% Sodium azide
Constituent: PBS -
Concentration information loading... -
精製度
Protein G purified -
特記事項（精製）
Purified from tissue culture supernatant. -
ポリ/モノ
モノクローナル -
クローン名
6A1 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab105922の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/200 - 1/1000. Predicted molecular weight: 57 kDa.
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| IHC-P | (3) |
1/200 - 1/1000.
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| 特記事項 |
|---|
|
WB
1/200 - 1/1000. Predicted molecular weight: 57 kDa. |
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IHC-P
1/200 - 1/1000. |
追加情報
Is unsuitable for Flow Cyt.
ターゲット情報
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機能
May participate in the regulation of T-cell-mediated immune response. May play a protective role in tumor cells by inhibiting natural-killer mediated cell lysis as well as a role of marker for detection of neuroblastoma cells. May be involved in the development of acute and chronic transplant rejection and in the regulation of lymphocytic activity at mucosal surfaces. Could also play a key role in providing the placenta and fetus with a suitable immunological environment throughout pregnancy. Both isoform 1 and isoform 2 appear to be redundant in their ability to modulate CD4 T-cell responses. Isoform 2 is shown to enhance the induction of cytotoxic T-cells and selectively stimulates interferon gamma production in the presence of T-cell receptor signaling. -
組織特異性
Ubiquitous but not detectable in peripheral blood lymphocytes or granulocytes. Weakly expressed in resting monocytes. Expressed in dendritic cells derived from monocytes. Expressed in epithelial cells of sinonasal tissue. Expressed in extravillous trophoblast cells and Hofbauer cells of the first trimester placenta and term placenta. -
配列類似性
Belongs to the immunoglobulin superfamily. BTN/MOG family.
Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
Contains 2 Ig-like V-type (immunoglobulin-like) domains. -
細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 80381 Human
- Omim: 605715 Human
- SwissProt: Q5ZPR3 Human
- Unigene: 77873 Human
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別名
- 4Ig B7 H3 antibody
- 4Ig-B7-H3 antibody
- AU016588 antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD276 antibody [6A1] (ab105922)ab105922, at a 1/200 dilution, staining CD276 in paraffin-embedded Human ovarian cancer tissues.
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![Western blot - Anti-CD276 antibody [6A1] (ab105922)](https://www.abcam.co.jp/ps/products/105/ab105922/Images/ab105922-118302-anti-cd276-antibody-6a1-western-blot.jpg)
Western blot - Anti-CD276 antibody [6A1] (ab105922)All lanes : Anti-CD276 antibody [6A1] (ab105922) at 1/200 dilution
Lane 1 : HEK293 cell lysate
Lane 2 : Transfected HEK293 cell lysate
Predicted band size: 57 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD276 antibody [6A1] (ab105922)](https://www.abcam.co.jp/ps/products/105/ab105922/Images/ab105922-118301-anti-cd276-antibody-6a1-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD276 antibody [6A1] (ab105922)ab105922, at a 1/200 dilution, staining CD276 in paraffin-embedded Human cervical cancer tissues. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD276 antibody [6A1] (ab105922)](https://www.abcam.co.jp/ps/products/105/ab105922/Images/ab105922-289423-anti-cd276-antibody-6a1-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD276 antibody [6A1] (ab105922)This image is courtesy of an anonymous Abreview.Formaldehyde-fixed, paraffin-embedded human tonsil tissue stained for CD276 using ab105922 at 1/800 dilution in immunohistochemical analysis.
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (9)
ab105922 は 9 報の論文で使用されています。
- Aicher WK et al. Expression patterns of the immune checkpoint ligand CD276 in urothelial carcinoma. BMC Urol 21:60 (2021). PubMed: 33845814
- Li H et al. MAPK-RAP1A Signaling Enriched in Hepatocellular Carcinoma Is Associated With Favorable Tumor-Infiltrating Immune Cells and Clinical Prognosis. Front Oncol 11:649980 (2021). PubMed: 34178637
- Deng J et al. Expression and Clinical Significance of Immune Checkpoint Regulator B7-H3 (CD276) in Human Meningioma. World Neurosurg 135:e12-e18 (2020). PubMed: 31629137
- Iida K et al. Prognostic impact of tumor-infiltrating CD276/Foxp3-positive lymphocytes and associated circulating cytokines in patients undergoing radical nephrectomy for localized renal cell carcinoma. Oncol Lett 17:4004-4010 (2019). PubMed: 30930997
- Zheng Y et al. High expression of B7-H2 or B7-H3 is associated with poor prognosis in hepatocellular carcinoma. Mol Med Rep 19:4315-4325 (2019). PubMed: 30942404
- Tang YC et al. Ginsenoside Rg3 targets cancer stem cells and tumor angiogenesis to inhibit colorectal cancer progression in vivo. Int J Oncol 52:127-138 (2018). PubMed: 29115601
- Xu ZL et al. B7-H3 promotes malignant progression of muscle-invasive bladder cancer. Oncol Rep 40:2722-2733 (2018). PubMed: 30132557
- Li Y et al. B7-H3 promotes the proliferation, migration and invasiveness of cervical cancer cells and is an indicator of poor prognosis. Oncol Rep 38:1043-1050 (2017). PubMed: 28627681
- Huang C et al. B7-H3, B7-H4, Foxp3 and IL-2 expression in cervical cancer: Associations with patient outcome and clinical significance. Oncol Rep 35:2183-90 (2016). PubMed: 26848032
