Anti-DPP4 抗体 [236.3] (ab119346)
Key features and details
- Mouse monoclonal [236.3] to DPP4
- Suitable for: ICC/IF, Flow Cyt
- Reacts with: Rat, Human
- Isotype: IgG2b
製品の概要
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製品名
Anti-DPP4 antibody [236.3]
DPP4 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [236.3] to DPP4 -
由来種
Mouse -
アプリケーション
適用あり: ICC/IF, Flow Cytmore details -
種交差性
交差種: Rat, Human -
免疫原
Full length protein corresponding to Rat DPP4. Full length native protein
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. -
バッファー
Constituent: 99% PBS -
Concentration information loading...
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精製度
Protein G purified -
ポリ/モノ
モノクローナル -
クローン名
236.3 -
アイソタイプ
IgG2b -
軽鎖の種類
kappa -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab119346の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC/IF |
1/20 - 1/200.
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Flow Cyt |
Use at an assay dependent concentration.
ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
特記事項 |
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ICC/IF
1/20 - 1/200. |
Flow Cyt
Use at an assay dependent concentration. ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
ターゲット情報
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機能
Cell surface glycoprotein receptor involved in the costimulatory signal essential for T-cell receptor (TCR)-mediated T-cell activation. Acts as a positive regulator of T-cell coactivation, by binding at least ADA, CAV1, IGF2R, and PTPRC. Its binding to CAV1 and CARD11 induces T-cell proliferation and NF-kappa-B activation in a T-cell receptor/CD3-dependent manner. Its interaction with ADA also regulates lymphocyte-epithelial cell adhesion. In association with FAP is involved in the pericellular proteolysis of the extracellular matrix (ECM), the migration and invasion of endothelial cells into the ECM. May be involved in the promotion of lymphatic endothelial cells adhesion, migration and tube formation. When overexpressed, enhanced cell proliferation, a process inhibited by GPC3. Acts also as a serine exopeptidase with a dipeptidyl peptidase activity that regulates various physiological processes by cleaving peptides in the circulation, including many chemokines, mitogenic growth factors, neuropeptides and peptide hormones. Removes N-terminal dipeptides sequentially from polypeptides having unsubstituted N-termini provided that the penultimate residue is proline. -
組織特異性
Expressed specifically in lymphatic vessels but not in blood vessels in the skin, small intestine, esophagus, ovary, breast and prostate glands. Not detected in lymphatic vessels in the lung, kidney, uterus, liver and stomach (at protein level). Expressed in the poorly differentiated crypt cells of the small intestine as well as in the mature villous cells. Expressed at very low levels in the colon. -
配列類似性
Belongs to the peptidase S9B family. DPPIV subfamily. -
ドメイン
The extracellular cysteine-rich region is necessary for association with collagen, dimer formation and optimal dipeptidyl peptidase activity. -
翻訳後修飾
The soluble form (Dipeptidyl peptidase 4 soluble form also named SDPP) derives from the membrane form (Dipeptidyl peptidase 4 membrane form also named MDPP) by proteolytic processing.
N- and O-Glycosylated.
Phosphorylated. Mannose 6-phosphate residues in the carbohydrate moiety are necessary for interaction with IGF2R in activated T-cells. Mannose 6-phosphorylation is induced during T-cell activation. -
細胞内局在
Cell membrane. Apical cell membrane. Cell projection > invadopodium membrane. Cell projection > lamellipodium membrane. Cell junction. Membrane raft. Translocated to the apical membrane through the concerted action of N- and O-Glycans and its association with lipid microdomains containing cholesterol and sphingolipids. Redistributed to membrane rafts in T-cell in a interleukin-12-dependent activation. Its interaction with CAV1 is necessary for its translocation to membrane rafts. Colocalized with PTPRC in membrane rafts. Colocalized with FAP in invadopodia and lamellipodia of migratory activated endothelial cells in collagenous matrix. Colocalized with FAP on endothelial cells of capillary-like microvessels but not large vessels within invasive breast ductal carcinoma. Colocalized with ADA at the cell junction in lymphocyte-epithelial cell adhesion. Colocalized with IGF2R in internalized cytoplasmic vesicles adjacent to the cell surface and Secreted. Detected in the serum and the seminal fluid. - Information by UniProt
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参照データベース
- Entrez Gene: 1803 Human
- Entrez Gene: 25253 Rat
- Omim: 102720 Human
- SwissProt: P27487 Human
- SwissProt: P14740 Rat
- Unigene: 368912 Human
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別名
- CD26 antigen antibody
- ADA-binding protein antibody
- ADABP antibody
see all
画像
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Flow cytometry analysis of CD26 in PC12 cells (green) compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x10^6 cells/ml, fixed with 2% paraformaldehyde and washed with PBS. Cells were blocked with a 2% solution of BSA-PBS for 30 min at room temperature and incubated with a CD26 monoclonal antibody (ab119346) at a dilution of 2 ug/test for 60 min at room temperature. Cells were then incubated for 40 min at room temperature in the dark using a Dylight 488-conjugated secondary antibody and re-suspended in PBS for FACS analysis.
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Flow cytometry analysis of CD26 in Hela cells (green) compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x10^6 cells/ml, fixed with 2% paraformaldehyde and washed with PBS. Cells were blocked with a 2% solution of BSA-PBS for 30 min at room temperature and incubated with a CD26 monoclonal antibody (ab119346) at a dilution of 2 ug/test for 60 min at room temperature. Cells were then incubated for 40 min at room temperature in the dark using a Dylight 488-conjugated secondary antibody and re-suspended in PBS for FACS analysis.
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ab119346 labelling CD26 (green) in the cytoplasm of HeLa cells (right) compared with a negative control (left) by Immunocytochemistry/Immunofluorescence. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with the primary antibody (1:20 in 3% BSA-PBS) overnight at 4 ºC. A DyLight 488-conjugated Goat anti-mouse IgG (H+L) was used as the secondary antibody. Red (phalloidin) - F-actin, Blue (DAPI) - nuclei. Images were taken at a magnification of 60x.
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ab119346 labelling CD26 (green) in the cytoplasm of PC12 cells (right) compared with a negative control (left) by Immunocytochemistry/Immunofluorescence. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with the primary antibody (1:20 in 3% BSA-PBS) overnight at 4 ºC. A DyLight 488-conjugated Goat anti-mouse IgG (H+L) was used as the secondary antibody. Red (phalloidin) - F-actin, Blue (DAPI) - nuclei. Images were taken at a magnification of 60x.
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ab119346 labelling CD26 (green) in the cytoplasm of H-4-II-E cells (right) compared with a negative control (left) by Immunocytochemistry/Immunofluorescence. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with the primary antibody (1:20 in 3% BSA-PBS) overnight at 4 ºC. A DyLight 488-conjugated Goat anti-mouse IgG (H+L) was used as the secondary antibody. Red (phalloidin) - F-actin, Blue (DAPI) - nuclei. Images were taken at a magnification of 60x.
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (5)
ab119346 は 5 報の論文で使用されています。
- Dosh RH et al. Interleukin 1 is a key driver of inflammatory bowel disease-demonstration in a murine IL-1Ra knockout model. Oncotarget 10:3559-3575 (2019). PubMed: 31191826
- Alsaadi EAJ et al. A Fusion Peptide in the Spike Protein of MERS Coronavirus. Viruses 11:N/A (2019). PubMed: 31491938
- Király K et al. Glial cell type-specific changes in spinal dipeptidyl peptidase 4 expression and effects of its inhibitors in inflammatory and neuropatic pain. Sci Rep 8:3490 (2018). IHC-P . PubMed: 29472575
- Zhang M et al. Expression levels of seprase/FAPa and DPPIV/CD26 in epithelial ovarian carcinoma. Oncol Lett 10:34-42 (2015). PubMed: 26170973
- Thompson NL et al. A Fischer rat substrain deficient in dipeptidyl peptidase IV activity makes normal steady-state RNA levels and an altered protein. Use as a liver-cell transplantation model. Biochem J 273 ( Pt 3):497-502 (1991). IHC, IP . PubMed: 1705112