Anti-CD25 抗体 [PC61.5] - Low endotoxin, Azide free (ab210333)

製品の概要

  • 製品名

    Anti-CD25 antibody [PC61.5] - Low endotoxin, Azide free
    CD25 一次抗体 製品一覧
  • 製品の詳細

    Rat monoclonal [PC61.5] to CD25 - Low endotoxin, Azide free
  • 由来種

    Rat
  • アプリケーション

    適用あり: Functional Studies, IHC-Fr, Flow Cyt, IPmore details
  • 種交差性

    交差種: Mouse
  • 免疫原

    The details of the immunogen for this antibody are not available.

  • 特記事項

    Endotoxin Level: Less than or equal to 0.01 EU/µg, as determined by the LaL assay.

    ab210333 is used as a marker for T cells, B cells and dendritic cell subsets. Expression of CD25, CD4 and the transcription factor Foxp3 is regarded as a phenotypic signature for Treg cells. As such, this antibody is widely used for depletion of Treg cells in vivo, as well as to distinguish Treg cells from naïve or conventional T cells which are CD25-.

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • バッファー

    pH: 7.2
    Constituents: 0.12% Monobasic dihydrogen sodium phosphate, 0.87% Sodium chloride
  • Concentration information loading...
  • 精製度

    Affinity purified
  • 特記事項(精製)

    Purity >90% by SDS-PAGE.
  • ポリ/モノ

    モノクローナル
  • クローン名

    PC61.5
  • アイソタイプ

    IgG1
  • 軽鎖の種類

    lambda

アプリケーション

Our Abpromise guarantee covers the use of ab210333 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Functional Studies Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.
IP Use at an assay dependent concentration.

ターゲット情報

  • 関連性

    The interleukin 2 (IL2) receptor alpha (IL2RA) (CD25) and beta (IL2RB) chains, together with the common gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric alpha chains (IL2RA) result in low-affinity receptor, while homodimeric beta (IL2RB) chains produce a medium-affinity receptor. Normally an integral-membrane protein, soluble CD25 has been isolated and determined to result from extracellular proteolyisis.
  • 細胞内局在

    Type I Cell Membrane
  • 参照データベース

  • 別名

    • IL2R antibody
    • IL2RA antibody
    • Interleukin 2 Receptor alpha antibody
    • TCGFR antibody

参考文献

ab210333 has not yet been referenced specifically in any publications.

レビューと Q&A

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Lymph Node)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate and Tris
Permeabilization
No
Specification
Lymph Node
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: 21°C
Fixative
Formaldehyde

Dr. Chris Gilbert

Verified customer

投稿 Aug 20 2018

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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