リコンビナントRabMAb

Anti-CD21 抗体 [EP3093] - BSA and Azide free (ab271855)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD21 antibody [EP3093] - BSA and Azide free (ab271855)
  • Immunocytochemistry/ Immunofluorescence - Anti-CD21 antibody [EP3093] - BSA and Azide free (ab271855)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD21 antibody [EP3093] - BSA and Azide free (ab271855)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD21 antibody [EP3093] - BSA and Azide free (ab271855)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD21 antibody [EP3093] - BSA and Azide free (ab271855)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD21 antibody [EP3093] - BSA and Azide free (ab271855)
  • Anti-CD21 antibody [EP3093] - BSA and Azide free (ab271855)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP3093] to CD21 - BSA and Azide free
  • Suitable for: ICC/IF, Flow Cyt, IHC-Fr, IHC-P, WB, IP
  • Reacts with: Mouse, Human

製品の概要

  • 製品名

    Anti-CD21 antibody [EP3093] - BSA and Azide free
    CD21 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EP3093] to CD21 - BSA and Azide free

  • 由来種

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    See all applications and species data

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • IHC-P: Human normal tonsil tissue. ICC: Raji cells. IP: Daudi cell lysate. Flow Cyt: Cynomolgus monkey whole blood. WB: Daudi and Raji cell lysate.

  • 特記事項

    Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

    ab271855 is the carrier-free version of ab75985. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • バッファー

    pH: 7.20
    Constituent: PBS

  • キャリア・フリー

    はい
  • Concentration information loading...

  • 精製度

    Affinity purified

  • ポリ/モノ

    モノクローナル

  • クローン名

    EP3093

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab271855 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

アプリケーション Species
ICC/IF
Human
IHC-P
Human
アプリケーション Abreviews 特記事項
ICC/IF
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent concentration.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 
IHC-Fr
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Predicted molecular weight: 113 kDa.
IP
Use at an assay dependent concentration.
特記事項
ICC/IF
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent concentration.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 
IHC-Fr
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Predicted molecular weight: 113 kDa.
IP
Use at an assay dependent concentration.

ターゲット情報

  • 機能

    Receptor for complement C3Dd, for the Epstein-Barr virus on human B-cells and T-cells and for HNRPU. Participates in B lymphocytes activation.

  • 組織特異性

    Mature B-lymphocytes, T-lymphocytes, pharyngeal epithelial cells, astrocytes and follicular dendritic cells of the spleen.

  • 関連疾患

    Genetic variations in CR2 are associated with susceptibility to systemic lupus erythematosus type 9 (SLEB9) [MIM:610927]. Systemic lupus erythematosus (SLE) is a chronic autoimmune disease with a complex genetic basis. SLE is an inflammatory, and often febrile multisystemic disorder of connective tissue characterized principally by involvement of the skin, joints, kidneys, and serosal membranes. It is thought to represent a failure of the regulatory mechanisms of the autoimmune system.

  • 配列類似性

    Belongs to the receptors of complement activation (RCA) family.
    Contains 15 Sushi (CCP/SCR) domains.

  • 細胞内局在

    Membrane.
  • Information by UniProt

  • 参照データベース

    • 別名

      • C3DR antibody
      • CD 21 antibody
      • CD21 antibody
      • Complement C3d receptor 2 antibody
      • Complement C3d receptor antibody
      • Complement component (3d/Epstein Barr virus) receptor 2 antibody
      • Complement receptor type 2 antibody
      • CR antibody
      • Cr2 antibody
      • CR2_HUMAN antibody
      • CVID7 antibody
      • EBV receptor antibody
      • EBV-R antibody
      • Epstein Barr virus receptor antibody
      • Epstein-Barr virus receptor antibody
      • EVBR antibody
      • SLEB9 antibody
      see all

    画像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD21 antibody [EP3093] - BSA and Azide free (ab271855)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD21 antibody [EP3093] - BSA and Azide free (ab271855)

      ab75985 showing positive staining in human normal tonsil tissue.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab75985).

    • Immunocytochemistry/ Immunofluorescence - Anti-CD21 antibody [EP3093] - BSA and Azide free (ab271855)
      Immunocytochemistry/ Immunofluorescence - Anti-CD21 antibody [EP3093] - BSA and Azide free (ab271855)

      ab75985 staining CD21 antibody in Raji (human Burkitt's lymphoma) cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 100% methanol. Samples were incubated with primary antibody at a dilution of 1/500. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a dilution of 1/1000.

      DAPI was used as a nuclear counterstain and the negative control was PBS only.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab75985).
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD21 antibody [EP3093] - BSA and Azide free (ab271855)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD21 antibody [EP3093] - BSA and Azide free (ab271855)

      ab75985 showing negative staining in human normal liver tissue.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab75985).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD21 antibody [EP3093] - BSA and Azide free (ab271855)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD21 antibody [EP3093] - BSA and Azide free (ab271855)

      ab75985 showing negative staining in human normal kidney tissue.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab75985).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD21 antibody [EP3093] - BSA and Azide free (ab271855)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD21 antibody [EP3093] - BSA and Azide free (ab271855)

      ab75985 showing negative staining in human normal brain tissue.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab75985).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD21 antibody [EP3093] - BSA and Azide free (ab271855)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD21 antibody [EP3093] - BSA and Azide free (ab271855)

      ab75985 showing negative staining in human normal breast tissue.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab75985).

    • Anti-CD21 antibody [EP3093] - BSA and Azide free (ab271855)
      Anti-CD21 antibody [EP3093] - BSA and Azide free (ab271855)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab271855 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab271855 は論文での使用が確認できていません。

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