Anti-CD200 / OX2 抗体 [OX-104] (ab23552)
Key features and details
- Mouse monoclonal [OX-104] to CD200 / OX2
- Suitable for: Flow Cyt
- Reacts with: Human
- Isotype: IgG1
Related conjugates and formulations
製品の概要
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製品名
Anti-CD200 / OX2 antibody [OX-104]
CD200 / OX2 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [OX-104] to CD200 / OX2 -
由来種
Mouse -
アプリケーション
適用あり: Flow Cytmore details -
種交差性
交差種: Human -
免疫原
Fusion protein corresponding to Human CD200/ OX2 (extracellular). Recombinant fusion protein consisting of the extracellular region of human CD200/ OX2 fused with domains three and four of rat CD4. Full details of the immunogen can be found in PubMed ID 11260322.
Database link: P41217 -
ポジティブ・コントロール
- Flow Cyt: Human peripheral blood mononuclear cells.
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特記事項
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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精製度
Protein G purified -
ポリ/モノ
モノクローナル -
クローン名
OX-104 -
アイソタイプ
IgG1 -
軽鎖の種類
kappa -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab23552の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
Flow Cyt |
Use a concentration of 1 µg/ml.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
特記事項 |
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Flow Cyt
Use a concentration of 1 µg/ml. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
ターゲット情報
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機能
Costimulates T-cell proliferation. May regulate myeloid cell activity in a variety of tissues. -
配列類似性
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
細胞内局在
Cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 4345 Human
- Omim: 155970 Human
- SwissProt: P41217 Human
- Unigene: 79015 Human
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別名
- Antigen identified by monoclonal antibody MRC OX 2 antibody
- CD200 antibody
- CD200 antigen antibody
see all
画像
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Human peripheral blood mononuclear cells stained with ab23552 (right) or mouse IgG1 kappa (ab170190) isotype (left). Human peripheral blood mononuclear cells were incubated for 30 min on ice in 1x PBS containing 10 µg/ml human IgG and 10 % normal goat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody (ab23552) or mouse IgG1 kappa (ab170190) (1x106 in 100 µl at 1 µg/ml) for 30 min on ice. The secondary antibody Goat anti-mouse IgG H&L (Alexa Fluor® 488, pre-adsorbed) (ab150177) was used at 1/2000 dilution for 30 min at 4°C. The cells were simultaneously stained with CD19 APC antibody.
Acquisition of >30,000 events were collected using a 50 mW Blue laser (488nm) and 530/30 bandpass filter. Events were gated on viable lymphocytes.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab23552 は 3 報の論文で使用されています。
- Zhou H et al. Feasibility of repairing full-thickness skin defects by iPSC-derived epithelial stem cells seeded on a human acellular amniotic membrane. Stem Cell Res Ther 10:155 (2019). PubMed: 31151466
- Adhikary G et al. Identification of a population of epidermal squamous cell carcinoma cells with enhanced potential for tumor formation. PLoS One 8:e84324 (2013). Human . PubMed: 24376802
- Rasmussen JG et al. Activation of Protease-Activated Receptor 2 Induces VEGF Independently of HIF-1. PLoS One 7:e46087 (2012). Flow Cyt ; Human . PubMed: 23049945