Anti-CD200 / OX2 抗体 [MRC OX-2] (ab22353)
![Flow Cytometry - Anti-CD200 / OX2 antibody [MRC OX-2] (ab22353)](https://www.abcam.co.jp/ps/products/22/ab22353/Images/ab22353-2-ab22353Flow1.jpg "Flow Cytometry - Anti-CD200 / OX2 antibody [MRC OX-2] (ab22353)")
Key features and details
- Mouse monoclonal [MRC OX-2] to CD200 / OX2
- Suitable for: Flow Cyt
- Reacts with: Rat
- Isotype: IgG1
Related conjugates and formulations
製品の概要
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製品名
Anti-CD200 / OX2 antibody [MRC OX-2]
CD200 / OX2 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [MRC OX-2] to CD200 / OX2 -
由来種
Mouse -
アプリケーション
適用あり: Flow Cytmore details -
種交差性
交差種: Rat -
免疫原
Tissue, cells or virus. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- Flow Cyt: Lewis rat splenocytes.
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特記事項
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
精製度
Protein G purified -
特記事項（精製）
Purified from TCS. -
一次抗体 備考
This antibody is a useful marker for the follicular dendritic cells in lymphoid tissues as it does not label the dendritic (interdigitating) cells in T areas that are Ia antigen positive. -
ポリ/モノ
モノクローナル -
クローン名
MRC OX-2 -
アイソタイプ
IgG1 -
軽鎖の種類
kappa -
研究分野
関連製品
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Alternative Versions
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab22353の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Flow Cyt |
Use a concentration of 0.2 µg/ml.
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| 特記事項 |
|---|
|
Flow Cyt
Use a concentration of 0.2 µg/ml. |
ターゲット情報
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機能
Costimulates T-cell proliferation. May regulate myeloid cell activity in a variety of tissues. -
配列類似性
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
細胞内局在
Cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 24560 Rat
- SwissProt: P04218 Rat
- Unigene: 7085 Rat
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別名
- Antigen identified by monoclonal antibody MRC OX 2 antibody
- CD200 antibody
- CD200 antigen antibody
see all
画像
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Flow Cytometry - Anti-CD200 / OX2 antibody [MRC OX-2] (ab22353)
Lewis rat splenocytes stained with ab22353 (right) or mouse IgG1κ (left). Lewis rat splenocytes were incubated for 30 min on ice in 10% rat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody (ab22353) or mouse IgG1κ Isotype (ab170190) (1x106 in 100µl at 0.2 µg/ml) for 30 min on ice.
The secondary antibody Goat Anti-Mouse IgG H&L (Alexa Fluor ® 488, pre-adsorbed) (ab150117) was used at 1/2000 dilution for 30 min at 4°C. The cells were simultaneously stained with CD3 APC antibody.
Acquisition of >30,000 events were collected using a 50 mW Blue laser (488nm) and 530/30 bandpass filter. Events were gated on viable lymphocytes.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab22353 は論文での使用が確認できていません。