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  1. Link

    cd20-antibody-l26-ab9475.pdf

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Immunology Adaptive Immunity B Cells CD
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Anti-CD20 抗体 [L26] (ab9475)

  • Datasheet
  • SDS
Reviews (7)Q&A (6)References (39)

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Abpromise

保証された製品品質、優れたカスタマー・サポート。 詳細はこちら。

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD20 antibody [L26] (ab9475)
  • Flow Cytometry - Anti-CD20 antibody [L26] (ab9475)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD20 antibody [L26] (ab9475)

Key features and details

  • Mouse monoclonal [L26] to CD20
  • Suitable for: IHC-P, Flow Cyt
  • Reacts with: Human
  • Isotype: IgG2a

こちらの製品もご検討ください

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Anti-Fas antibody [EPR5700] - BSA and Azide free (ab178076)
二次抗体
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Goat Anti-Mouse IgG H&L (HRP) (ab205719)

関連製品

製品の概要

  • 製品名

    Anti-CD20 antibody [L26]
    CD20 一次抗体 製品一覧
  • 製品の詳細

    Mouse monoclonal [L26] to CD20
  • 由来種

    Mouse
  • アプリケーション

    適用あり: IHC-P, Flow Cytmore details
  • 種交差性

    交差種: Human
  • 免疫原

    Tissue, cells or virus corresponding to Human CD20. Human tonsil B cells.

  • ポジティブ・コントロール

    • Tonsil.
  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー

    pH: 7.30
    Preservative: 0.1% Sodium azide

    Contains PBS, BSA, Tween
  • Concentration information loading...
  • 精製度

    Tissue culture supernatant
  • ポリ/モノ

    モノクローナル
  • クローン名

    L26
  • ミエローマ

    unknown
  • アイソタイプ

    IgG2a
  • 軽鎖の種類

    kappa
  • 研究分野

    • Immunology
    • Adaptive Immunity
    • B Cells
    • CD
    • Stem Cells
    • Hematopoietic Progenitors
    • Hematopoietic Stem Cells
    • Human Lineage Negative
    • Cancer
    • Tumor immunology
    • Tumor-associated antigens
    • Neuroscience
    • Processes

関連製品

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG2a, kappa monoclonal [MG2a-53] - Isotype control (ab18415)

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab9475の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IHC-P (4)
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt
Use 0.1µg for 106 cells.

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

 

特記事項
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt
Use 0.1µg for 106 cells.

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

 

ターゲット情報

  • 機能

    This protein may be involved in the regulation of B-cell activation and proliferation.
  • 組織特異性

    Expressed on B-cells.
  • 関連疾患

    Defects in MS4A1 are the cause of immunodeficiency common variable type 5 (CVID5) [MIM:613495]; also called antibody deficiency due to CD20 defect. CVID5 is a primary immunodeficiency characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections and an inability to mount an antibody response to antigen. The defect results from a failure of B-cell differentiation and impaired secretion of immunoglobulins; the numbers of circulating B cells is usually in the normal range, but can be low.
  • 配列類似性

    Belongs to the MS4A family.
  • 翻訳後修飾

    Phosphorylated. Might be functionally regulated by protein kinase(s).
  • 細胞内局在

    Membrane.
  • Target information above from: UniProt accession P11836 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 参照データベース

    • Entrez Gene: 931 Human
    • Omim: 112210 Human
    • SwissProt: P11836 Human
    • Unigene: 712553 Human
    • 別名

      • APY antibody
      • ATOPY antibody
      • B lymphocyte antigen CD20 antibody
      • B Lymphocyte Cell Surface Antigen B1 antibody
      • B-lymphocyte antigen CD20 antibody
      • B-lymphocyte cell-surface antigen B1 antibody
      • B-lymphocyte surface antigen B1 antibody
      • B1 antibody
      • Bp 35 antibody
      • Bp35 antibody
      • CD 20 antibody
      • CD20 antibody
      • CD20 antigen antibody
      • CD20 receptor antibody
      • CD20_HUMAN antibody
      • CVID 5 antibody
      • CVID5 antibody
      • Fc epsilon receptor I beta chain antibody
      • Fc Fragment of IgE high affinity I receptor for beta polypeptide antibody
      • FCER1B antibody
      • High affinity immunoglobulin epsilon receptor subunit beta antibody
      • IgE Fc receptor subunit beta antibody
      • IGEL antibody
      • IGER antibody
      • IGHER antibody
      • LEU 16 antibody
      • Leu-16 antibody
      • LEU16 antibody
      • leukocyte surface antigen Leu 16 antibody
      • Leukocyte surface antigen Leu-16 antibody
      • Ly44 antibody
      • Membrane spanning 4 domains A1 antibody
      • Membrane spanning 4 domains subfamily A member 2 antibody
      • membrane-spanning 4-domains A1 antibody
      • Membrane-spanning 4-domains subfamily A member 1 antibody
      • MGC3969 antibody
      • MS4A1 antibody
      • MS4A2 antibody
      • S7 antibody
      see all

    画像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD20 antibody [L26] (ab9475)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD20 antibody [L26] (ab9475)
      Formalin-fixed, paraffin-embeded human tonsil tissue stained for CD20 using ab9475 at 1/50 dilution in immunohistochemical analysis. Antigen retrieval with citrate buffer pH 6.0.
    • Flow Cytometry - Anti-CD20 antibody [L26] (ab9475)
      Flow Cytometry - Anti-CD20 antibody [L26] (ab9475)

      Human peripheral blood lymphocytes stained with ab9475 (red line). Human whole blood was processed using a modified protocol based on Chow et al, 2005 (PMID: 16080188). In brief, human whole blood was fixed in 4% formaldehyde (methanol-free) for 10 min at 22°C. Red blood cells were then lyzed by the addition of Triton X-100 (final concentration - 0.1%) for 15 min at 37°C. For experimentation, cells were treated with 50% methanol (-20°C) for 15 min at 4°C. Cells were then incubated with the antibody (ab9475, 0.1μg/1x106 cells) for 30 min at 4°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 4°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >30,000 total events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. Gating strategy - peripheral blood lymphocytes.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD20 antibody [L26] (ab9475)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD20 antibody [L26] (ab9475)

      ab9475 - immunohistochemistry

      Formalin fixed paraffin embedded human tonsil stained with CD20, B cell using ABC and Fast Red chromagen.

    プロトコール

    • Flow cytometry protocols
    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    データシートおよび資料

    • SDS download

    • Datasheet download

      Download

    参考文献 (39)

    ab9475 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab9475 は 39 報の論文で使用されています。

    • Stone D  et al. Gene Transfer in Adeno-Associated Virus Seropositive Rhesus Macaques Following Rapamycin Treatment and Subcutaneous Delivery of AAV6, but Not Retargeted AAV6 Vectors. Hum Gene Ther 32:96-112 (2021). PubMed: 32998579
    • Gaudio E  et al. Targeting CD205 with the antibody drug conjugate MEN1309/OBT076 is an active new therapeutic strategy in lymphoma models. Haematologica 105:2584-2591 (2020). PubMed: 33131247
    • Huang M  et al. The Influence of Immune Heterogeneity on the Effectiveness of Immune Checkpoint Inhibitors in Multifocal Hepatocellular Carcinomas. Clin Cancer Res 26:4947-4957 (2020). PubMed: 32527942
    • Hambraeus M  et al. Differential Activation of Immune Effector Processes in Mature Compared to Immature Sacrococcygeal Teratomas. Fetal Pediatr Pathol N/A:1-13 (2020). PubMed: 33063585
    • Mei Q  et al. Camrelizumab Plus Gemcitabine, Vinorelbine, and Pegylated Liposomal Doxorubicin in Relapsed/Refractory Primary Mediastinal B-Cell Lymphoma: A Single-Arm, Open-Label, Phase II Trial. Clin Cancer Res 26:4521-4530 (2020). PubMed: 32499235
    View all Publications for this product

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-10 of 13 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD20 antibody [L26]

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Frontal Cortex (Brain))
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Tris-EDTA
    Permeabilization
    Yes - .1% TritonX-100
    Specification
    Frontal Cortex (Brain)
    Blocking step
    (agent) for 30 minute(s) · Concentration: 3% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    投稿 Mar 27 2020

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD20 antibody [L26]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (tonsil)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Tris/EDTA pH 9.0
    Permeabilization
    No
    Specification
    tonsil
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 15% · Temperature: RT°C
    Fixative
    10% NBF
    Read More

    Abcam user community

    Verified customer

    投稿 Aug 12 2019

    Western blot abreview for Anti-CD20 antibody [L26]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Lymphocytes)
    Gel Running Conditions
    Reduced Denaturing (4-12% Bis-Tris)
    Loading amount
    20 µg
    Specification
    Lymphocytes
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    投稿 Sep 15 2018

    Immunocytochemistry/ Immunofluorescence abreview for Anti-CD20 antibody [L26]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.5% · Temperature: 22°C
    Sample
    Human Cell (Daudi and Jurkat)
    Specification
    Daudi and Jurkat
    Permeabilization
    Yes - 0.5% Triton X-100
    Fixative
    Formaldehyde
    Read More

    MS. Natalie Erdmann

    Verified customer

    投稿 Mar 19 2015

    Question





    In which domain of the protein is the immunogen localised?
     



     




     



    Patrick Franken





    Sr. Research Associate




    Genmab B.V. · Yalelaan 60 · 3584 CM Utrecht · P.O. Box 85199 · 3508 AD Utrecht

    The Netherlands · Main +31 30 2 123 123 · Direct +31 30 2 123 342 

    Cell +31 6 5798 5139 · CC no. 30169902



    Help protect our environment - only print this message if necessary.

    Read More

    Abcam community

    Verified customer

    Asked on Nov 16 2017

    Answer



    The localization of the epitope is located in the cytoplasmic domain.



    Read More

    Abcam Scientific Support

    Answered on Nov 16 2017

    Question

    One doubt: some of this antibody only react with mouse, and you suggested human tissue. Is it correct?
    Thanks,

    Read More

    Abcam community

    Verified customer

    Asked on Aug 14 2012

    Answer

    Thank you for your email.

    There may be some antibodies where I might have suggested the human tissues or cells. I apologize for this. I have included the positive control information irrespective of species so similar mouse tissues sections can used as positive control.

    Actually the positive control information for each antibody is present on the datasheet; please check the datasheet. Alternatively, we always follow human protein Atlas database (http://www.proteinatlas.org/index.php). The second option is, searching positive control for other antibodies in catalogue against same target; the cell line or tissue sections tested with those antibodies could also be used as positive control. The third place to search for is literature.

    I hope this information will be helpful. Should you have any question please do not hesitate to contact me.

    Read More

    Abcam Scientific Support

    Answered on Aug 15 2012

    Question

    Our customer would like to know which are the best positive control
    and negative control for the antibodies below.
    1. anti Natural killer - ab36392
    2. anti- interferon gama - ab9918
    3. anti TNF alfa - ab34674
    4. anti TGF beta - ab15537
    5. anti neutrófilo - ab2557
    6. anti CD20 - ab9475
    7. anti células T CD8 - ab22378
    8. anti células T CD4 - ab51312
    She will do ICC and IHC-P assays.
    Thanks and best regards,

    Read More

    Abcam community

    Verified customer

    Asked on Aug 10 2012

    Answer

    Thank you for contacting us.

    The positive control for ab36392 is IL2 activatedcultured NK cells.

    ab9918; human tonsil sections, human cervical squamous cell carcinoma section

    ab34674; human artery or colon tissue sections

    ab15537; HepG2 cells. mouse brain sections, human placenta sections; Human hepatocellular liver carcinoma cell line; Human hepatocarcinoma tissue sections

    ab2557; mouse spleen tissue sections

    ab9475; human tonsil tissue sections

    ab22378; mouse spleen tissue sections

    ab51312; Thymus tissue sections, human pancreas tissue sections.

    The best database to search for positive control is Human Protein Atlas. Please click the link http://www.proteinatlas.org/index.php

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Use our products? Submit an Abreview. Earn rewards!
    https://www.abcam.com/abreviews

    Read More

    Abcam Scientific Support

    Answered on Aug 10 2012

    Question

    what is the concentration of this product? ab9475 250 µl

    Read More

    Abcam community

    Verified customer

    Asked on Jul 11 2012

    Answer

    Thank you for contacting Abcam.

    The antibody ab9475, is supplied as a tissue culture supernatant, not a as a purified antibody. Due to this we are unable to determine the amount of antibody present within the sample, as other non-specific proteins will also be present.

    If there is anything else I can help you with, please let me know

    Read More

    Abcam Scientific Support

    Answered on Jul 11 2012

    Question

    Hi.I made a 1:500 dilution in TBST on the primary antibody:
    Anti-CD20 antibody [L26] (ab9475)
    How many times can I reuse it?

    Read More

    Abcam community

    Verified customer

    Asked on Feb 29 2012

    Answer

    Thank you for your inquiry.

    When stored at +4C you can use the antibody dilution several times in the same application (about 3-5x). If you encounter any difficulties though, it is recommended to use a fresh aliquot/dilution of the primary antibody.

    I hope this information is helpful to you and wish you the best of luck with your research. Please do not hesitate to contact us if you have any questions.

    Read More

    Abcam Scientific Support

    Answered on Feb 29 2012

    Question

    HI

    What is your phone number.

    Do you have CD20 antigen & CD20 monoclonal antibody?

    Read More

    Abcam community

    Verified customer

    Asked on Feb 15 2012

    Answer

    Thank you for your enquiry.

    We have a number of monoclonal antibodies to CD20. We do not have a full length CD20 protein but we do have 2 small peptides derived from CD20, these are catalog numbers ab97360 and ab22803.

    Feel free to call us at 888-77-ABCAM(22226). My name is Jeremy so feel free to ask for me when you call.

    Read More

    Abcam Scientific Support

    Answered on Feb 15 2012

    1-10 of 13 Abreviews or Q&A

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