Anti-CD20 抗体 [GOT214A] - BSA and Azide free (ab271298)
![Immunocytochemistry/ Immunofluorescence - Anti-CD20 antibody [GOT214A] - BSA and Azide free (ab271298)](https://www.abcam.co.jp/ps/products/271/ab271298/Images/ab271298-2-anti-cd20-antibody-got214a-immunocytochemistry-mouse-pbmc.jpg "Immunocytochemistry/ Immunofluorescence - Anti-CD20 antibody [GOT214A] - BSA and Azide free (ab271298)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rat monoclonal [GOT214A] to CD20 - BSA and Azide free
- Suitable for: ICC/IF, IHC-P
- Reacts with: Mouse
製品の概要
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製品名
Anti-CD20 antibody [GOT214A] - BSA and Azide free
CD20 一次抗体 製品一覧 -
製品の詳細
Rat monoclonal [GOT214A] to CD20 - BSA and Azide free -
由来種
Rat -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF MouseIHC-P Mouse
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免疫原
Tissue, cells or virus. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- IHC-P: Mouse spleen tissue. ICC: Mouse PBMC cells.
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特記事項
ab271298 is the carrier-free version of ab271288. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
ab271298 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
バッファー
pH: 7.2
Constituent: PBS -
キャリア・フリー
はい -
Concentration information loading... -
精製度
Ion Exchange Chromatography -
ポリ/モノ
モノクローナル -
クローン名
GOT214A -
アイソタイプ
IgG2a -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Related Products
アプリケーション
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab271298 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| アプリケーション | Species |
|---|---|
| ICC/IF |
Mouse
|
| IHC-P |
Mouse
|
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF |
Use at an assay dependent concentration.
|
|
| IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
| 特記事項 |
|---|
|
ICC/IF
Use at an assay dependent concentration. |
|
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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機能
This protein may be involved in the regulation of B-cell activation and proliferation. -
組織特異性
Expressed on B-cells. -
関連疾患
Defects in MS4A1 are the cause of immunodeficiency common variable type 5 (CVID5) [MIM:613495]; also called antibody deficiency due to CD20 defect. CVID5 is a primary immunodeficiency characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections and an inability to mount an antibody response to antigen. The defect results from a failure of B-cell differentiation and impaired secretion of immunoglobulins; the numbers of circulating B cells is usually in the normal range, but can be low. -
配列類似性
Belongs to the MS4A family. -
翻訳後修飾
Phosphorylated. Might be functionally regulated by protein kinase(s). -
細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 12482 Mouse
- SwissProt: P19437 Mouse
- Unigene: 4046 Mouse
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別名
- APY antibody
- ATOPY antibody
- B lymphocyte antigen CD20 antibody
see all
画像
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Immunocytochemistry/ Immunofluorescence - Anti-CD20 antibody [GOT214A] - BSA and Azide free (ab271298)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Mouse PBMC cells labelling CD20 with ab271288 at 1/50 (16.04 μg/mL) dilution, followed by ab150157 Goat Anti-Rat IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing membranous staining in some subset of mouse PBMC cell line. ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/200 dilution, followed by ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) at 1/500 dilution(Red). The Nuclear counterstain was DAPI (Blue).
-ve control 1: Used ab271288 at 1/50 (16.04 μg/mL) dilution, followed by ab150080 at 1/500 dilution.
-ve control 2: Used ab179513 at 1/200 dilution, followed by ab150157 at 1/1000 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab271288).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD20 antibody [GOT214A] - BSA and Azide free (ab271298)](https://www.abcam.co.jp/ps/products/271/ab271298/Images/ab271298-1-anti-cd20-antibody-got214a-immunohistochemistry-mouse-spleen.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD20 antibody [GOT214A] - BSA and Azide free (ab271298)Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling CD20 with ab271288 at 1/100 (8.02 μg/mL) dilution followed by a ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882). Membranous staining on the mouse spleen is observed. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882).
Heat mediated antigen retrieval using ab93678 (citrate buffer, pH 6.0).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab271288).
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![Anti-CD20 antibody [GOT214A] - BSA and Azide free (ab271298)](https://www.abcam.co.jp/ps/products/271/ab271298/Images/ab271298-3-benefits-of-recombinant-antibodies.png)
Anti-CD20 antibody [GOT214A] - BSA and Azide free (ab271298)
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
参考文献 (0)
ab271298 は論文での使用が確認できていません。
