PerCP Anti-CD20 抗体 [2H7] (ab114860)
Key features and details
- PerCP Mouse monoclonal [2H7] to CD20
- Suitable for: Flow Cyt
- Reacts with: Human, Non human primates
- Conjugation: PerCP. Ex: 482nm, Em: 675nm
- Isotype: IgG2b
製品の概要
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製品名
PerCP Anti-CD20 antibody [2H7]
CD20 一次抗体 製品一覧 -
製品の詳細
PerCP Mouse monoclonal [2H7] to CD20 -
由来種
Mouse -
標識
PerCP. Ex: 482nm, Em: 675nm -
アプリケーション
適用あり: Flow Cytmore details -
種交差性
交差種: Human, Non human primates -
免疫原
Tissue, cells or virus corresponding to Human CD20. Human tonsillar B cells.
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ポジティブ・コントロール
- Flow Cyt: Human peripheral blood cells.
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特記事項
The mouse monoclonal antibody 2H7 recognizes CD20 (B1, Bp35), a 33-37 kDa non-glycosylated membrane receptor with four transmembrane domains, expressed on pre-B lymphocytes, resting and activated B cells (not plasma cells), follicular dendritic cells, and at low levels on peripheral blood T lymphocytes.
Purified antibody is conjugated with Peridinin-chlorophyll-protein complex (PerCP) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
pH: 7.4
Preservative: 0.1% Sodium azide
Constituents: 99% PBS, 0.2% BSA -
Concentration information loading...
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精製度
Size exclusion -
一次抗体 備考
The mouse monoclonal antibody 2H7 recognizes CD20 (B1, Bp35), a 33-37 kDa non-glycosylated membrane receptor with four transmembrane domains, expressed on pre-B lymphocytes, resting and activated B cells (not plasma cells), follicular dendritic cells, and at low levels on peripheral blood T lymphocytes. -
ポリ/モノ
モノクローナル -
クローン名
2H7 -
アイソタイプ
IgG2b -
研究分野
関連製品
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Alternative Versions
- PE Anti-CD20 antibody [2H7] (ab112461)
- Biotin Anti-CD20 antibody [2H7] (ab123487)
- PerCP/Cy5.5® Anti-CD20 antibody [2H7] (ab157321)
- PE/DyLight™ 594 Anti-CD20 antibody [2H7] (ab187593)
- PE/Cy7® Anti-CD20 antibody [2H7] (ab234204)
- Alexa Fluor® 700 Anti-CD20 antibody [2H7] (ab269315)
- FITC Anti-CD20 antibody [2H7] (ab51792)
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab114860の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
Flow Cyt |
Use 10µl for 106 cells.
(or 100 µl of whole blood). ab172890 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
特記事項 |
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Flow Cyt
Use 10µl for 106 cells. (or 100 µl of whole blood). ab172890 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
ターゲット情報
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機能
This protein may be involved in the regulation of B-cell activation and proliferation. -
組織特異性
Expressed on B-cells. -
関連疾患
Defects in MS4A1 are the cause of immunodeficiency common variable type 5 (CVID5) [MIM:613495]; also called antibody deficiency due to CD20 defect. CVID5 is a primary immunodeficiency characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections and an inability to mount an antibody response to antigen. The defect results from a failure of B-cell differentiation and impaired secretion of immunoglobulins; the numbers of circulating B cells is usually in the normal range, but can be low. -
配列類似性
Belongs to the MS4A family. -
翻訳後修飾
Phosphorylated. Might be functionally regulated by protein kinase(s). -
細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 931 Human
- Omim: 112210 Human
- SwissProt: P11836 Human
- Unigene: 712553 Human
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別名
- APY antibody
- ATOPY antibody
- B lymphocyte antigen CD20 antibody
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データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab114860 は 2 報の論文で使用されています。
- Deans JP et al. Rapid redistribution of CD20 to a low density detergent-insoluble membrane compartment. J Biol Chem 273:344-8 (1998). PubMed: 9417086
- Deans JP et al. Association of 75/80-kDa phosphoproteins and the tyrosine kinases Lyn, Fyn, and Lck with the B cell molecule CD20. Evidence against involvement of the cytoplasmic regions of CD20. J Biol Chem 270:22632-8 (1995). PubMed: 7545683