Anti-CD2 抗体 [SP304] (ab227698)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [SP304] to CD2
- Suitable for: IHC-P
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-CD2 antibody [SP304]
CD2 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [SP304] to CD2 -
由来種
Rabbit -
アプリケーション
適用あり: IHC-Pmore details -
種交差性
交差種: Human -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- IHC-P: Human tonsil, thymus, colon, stomach, stomach adenocarcinoma, colon adenocarcinoma, lung squamous cell carcinoma, lung adenocarcinoma, uterus and cervical squamous cell carcinoma tissue.
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特記事項
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading...
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精製度
Protein A/G purified -
特記事項(精製)
Purified from TCS by protein A/G. -
ポリ/モノ
モノクローナル -
クローン名
SP304 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab227698の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P |
1/100.
Boil tissue section in EDTA buffer, pH 8.0 for 10 minutes followed by cooling at room temperature for 20 minutes. Incubate with primary antibody for 10 minutes at room temperature. |
特記事項 |
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IHC-P
1/100. Boil tissue section in EDTA buffer, pH 8.0 for 10 minutes followed by cooling at room temperature for 20 minutes. Incubate with primary antibody for 10 minutes at room temperature. |
ターゲット情報
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機能
CD2 interacts with lymphocyte function-associated antigen (LFA-3) and CD48/BCM1 to mediate adhesion between T-cells and other cell types. CD2 is implicated in the triggering of T-cells, the cytoplasmic domain is implicated in the signaling function. -
配列類似性
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 914 Human
- Omim: 186990 Human
- SwissProt: P06729 Human
- Unigene: 523500 Human
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別名
- CD 2 antibody
- CD2 antibody
- CD2 antigen (p50), sheep red blood cell receptor antibody
see all
画像
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Immunohistochemistry analysis of paraffin-embedded human tonsil tissue sections labelling CD2 with ab227698 at 1/100 dilution. The section was incubated with ab227698 for 10 mins at room temperature. LeicaDS9800 (Bond™ Polymer Refine Detection) was used as the secondary antibody. Sections were counterstained with Hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
Positive staining on the human tonsil tissue, performed on a Leica Biosystems BOND® RX instrument. -
Formalin-fixed, paraffin-embedded human tonsil tissue stained for CD2 using ab227698 at 1/100 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human tonsil tissue stained for CD2 using ab227698 at 1/100 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human thymus tissue stained for CD2 using ab227698 at 1/100 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human colon tissue stained for CD2 using ab227698 at 1/100 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human stomach tissue stained for CD2 using ab227698 at 1/100 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human stomach adenocarcinoma tissue stained for CD2 using ab227698 at 1/100 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human colon adenocarcinoma tissue stained for CD2 using ab227698 at 1/100 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human lung squamous cell carcinoma tissue stained for CD2 using ab227698 at 1/100 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human lung adenocarcinoma tissue stained for CD2 using ab227698 at 1/100 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human uterus tissue stained for CD2 using ab227698 at 1/100 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human cervical squamous cell carcinoma tissue stained for CD2 using ab227698 at 1/100 dilution in immunohistochemical analysis.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab227698 は論文での使用が確認できていません。