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Anti-CD1c 抗体 [EPR23189-196] - BSA and Azide free (ab270797)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD1c antibody [EPR23189-196] - BSA and Azide free (ab270797)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD1c antibody [EPR23189-196] - BSA and Azide free (ab270797)
  • Anti-CD1c antibody [EPR23189-196] - BSA and Azide free (ab270797)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23189-196] to CD1c - BSA and Azide free
  • Suitable for: WB, IHC-P
  • Reacts with: Human

製品の概要

  • 製品名

    Anti-CD1c antibody [EPR23189-196] - BSA and Azide free
    CD1c 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR23189-196] to CD1c - BSA and Azide free

  • 由来種

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    See all applications and species data

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: Jurkat and MOLT-4 whole cell lysates; Human thymus, tonsil and spleen tissue lysates. IHC-P: Human spleen and Hodgkin's lymphoma tissue.

  • 特記事項

    ab270797 is the carrier-free version of ab246520. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • バッファー

    pH: 7.2
    Constituent: PBS

  • キャリア・フリー

    はい
  • Concentration information loading...

  • 精製度

    Protein A purified

  • ポリ/モノ

    モノクローナル

  • クローン名

    EPR23189-196

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab270797 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

アプリケーション Species
IHC-P
Human
アプリケーション Abreviews 特記事項
WB
Use at an assay dependent concentration. Detects a band of approximately 43 kDa (predicted molecular weight: 38 kDa).
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
特記事項
WB
Use at an assay dependent concentration. Detects a band of approximately 43 kDa (predicted molecular weight: 38 kDa).
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • 追加情報
    Is unsuitable for Flow Cyt,ICC/IF or IP.

    • ターゲット情報

    • 機能

      Antigen-presenting protein that binds self and non-self lipid and glycolipid antigens and presents them to T-cell receptors on natural killer T-cells.

    • 組織特異性

      Expressed on cortical thymocytes, on certain T-cell leukemias, and in various other tissues.

    • 配列類似性

      Contains 1 Ig-like (immunoglobulin-like) domain.

    • 細胞内局在

      Cell membrane. Endosome membrane. Subject to intracellular trafficking between the cell membrane and endosomes.
    • Information by UniProt

    • 参照データベース

      • 別名

        • BDCA1 antibody
        • CD1 antibody
        • CD1A antibody
        • CD1c antibody
        • CD1c antigen antibody
        • CD1C antigen c polypeptide antibody
        • CD1c molecule antibody
        • CD1C_HUMAN antibody
        • Cortical thymocyte antigen CD1C antibody
        • Differentiation antigen CD1 alpha 3 antibody
        • R7 antibody
        • T cell surface glycoprotein CD1c antibody
        • T-cell surface glycoprotein CD1c antibody
        see all

      画像

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD1c antibody [EPR23189-196] - BSA and Azide free (ab270797)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD1c antibody [EPR23189-196] - BSA and Azide free (ab270797)

        Immunohistochemical analysis of paraffin-embedded Human Hodgkin's lymphoma tissue labeling CD1c with ab246520 at 1/1000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human Hodgkin's lymphoma. The section was incubated with ab246520 for 10 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

        Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

        Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

        This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab246520).

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD1c antibody [EPR23189-196] - BSA and Azide free (ab270797)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD1c antibody [EPR23189-196] - BSA and Azide free (ab270797)

        Immunohistochemical analysis of paraffin-embedded Human spleen tissue labeling CD1c with ab246520 at 1/1000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human spleen. (PMID: 15191950)The section was incubated with ab246520 for 10 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

        Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

        Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

        This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab246520).

      • Anti-CD1c antibody [EPR23189-196] - BSA and Azide free (ab270797)
        Anti-CD1c antibody [EPR23189-196] - BSA and Azide free (ab270797)

      プロトコール

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      参考文献 (0)

      ab270797 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

      ab270797 は論文での使用が確認できていません。

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