Anti-CD1b 抗体 [EP7251] (ab173576)
Key features and details
- Rabbit monoclonal [EP7251] to CD1b
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Related conjugates and formulations
製品の概要
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製品名
Anti-CD1b antibody [EP7251]
CD1b 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EP7251] to CD1b -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-P, ICC/IFmore details
適用なし: Flow Cyt or IP -
種交差性
交差種: Human
非交差種: Mouse, Rat -
免疫原
Synthetic peptide within Human CD1b aa 50-150 (Cysteine residue). The exact sequence is proprietary.
Database link: P29016 -
ポジティブ・コントロール
- Fetal thymus and Jurkat lysates, Human spleen tissue, Jurkat cells.
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特記事項
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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精製度
Tissue culture supernatant -
ポリ/モノ
モノクローナル -
クローン名
EP7251 -
アイソタイプ
IgG -
研究分野
関連製品
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Isotype control
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab173576の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB |
1/1000 - 1/10000. Predicted molecular weight: 37 kDa.
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IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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ICC/IF |
1/50 - 1/100.
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特記事項 |
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WB
1/1000 - 1/10000. Predicted molecular weight: 37 kDa. |
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
ICC/IF
1/50 - 1/100. |
ターゲット情報
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機能
Antigen-presenting protein that binds self and non-self lipid and glycolipid antigens and presents them to T-cell receptors on natural killer T-cells. -
組織特異性
Expressed on cortical thymocytes, on certain T-cell leukemias, and in various other tissues. -
配列類似性
Contains 1 Ig-like (immunoglobulin-like) domain. -
細胞内局在
Cell membrane. Endosome membrane. Lysosome membrane. Subject to intracellular trafficking between the cell membrane, endosomes and lysosomes. Localizes to cell surface lipid rafts. - Information by UniProt
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参照データベース
- Entrez Gene: 910 Human
- Omim: 188360 Human
- SwissProt: P29016 Human
- Unigene: 1310 Human
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別名
- A1 domain antibody
- CD1 antibody
- CD1A antibody
see all
画像
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Immunohistochemical analysis of paraffin-embedded Human spleen tissue labeling CD1b with ab173576 at 1/100 dilution
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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ab173576 showing +ve staining in Human normal thymus tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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ab173576 showing +ve staining in Human normal tonsil tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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ab173576 showing -ve staining in Human normal pancreas tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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ab173576 showing -ve staining in Human skeletal muscle tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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ab173576 showing -ve staining in Human endometrial adenocarcinoma tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Immunofluorescent staining of Jurkat cells labeling CD1b with ab173576 at 1/50 dilution
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All lanes : Anti-CD1b antibody [EP7251] (ab173576) at 1/1000 dilution
Lane 1 : Human fetal thymus lysate
Lane 2 : Jurkat lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 37 kDa
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab173576 は 1 報の論文で使用されています。
- Li C et al. Comprehensive Profiling Reveals Distinct Microenvironment and Metabolism Characterization of Lung Adenocarcinoma. Front Genet 12:619821 (2021). PubMed: 34122500