Anti-CD19 抗体 [MB19-1] (ab25177)
Key features and details
- Mouse monoclonal [MB19-1] to CD19
- Suitable for: Flow Cyt
- Reacts with: Mouse
- Isotype: IgA
Related conjugates and formulations
製品の概要
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製品名
Anti-CD19 antibody [MB19-1]
CD19 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [MB19-1] to CD19 -
由来種
Mouse -
アプリケーション
適用あり: Flow Cytmore details -
種交差性
交差種: Mouse -
免疫原
Tissue, cells or virus corresponding to Mouse CD19. Mouse CD19 cDNA-transfected mouse 300.19 cells
Database link: P25918 -
ポジティブ・コントロール
- Flow Cyt: Mouse splenocytes
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特記事項
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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特記事項(精製)
Diafiltration. -
ポリ/モノ
モノクローナル -
クローン名
MB19-1 -
アイソタイプ
IgA -
研究分野
関連製品
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Alternative Versions
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab25177の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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Flow Cyt |
Use a concentration of 1 - 5 µg/ml.
ab37322 - Mouse monoclonal IgA, is suitable for use as an isotype control with this antibody. |
特記事項 |
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Flow Cyt
Use a concentration of 1 - 5 µg/ml. ab37322 - Mouse monoclonal IgA, is suitable for use as an isotype control with this antibody. |
ターゲット情報
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機能
Assembles with the antigen receptor of B lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation. -
関連疾患
Defects in CD19 are the cause of immunodeficiency common variable type 3 (CVID3) [MIM:613493]; also called antibody deficiency due to CD19 defect. CVID3 is a primary immunodeficiency characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections and an inability to mount an antibody response to antigen. The defect results from a failure of B-cell differentiation and impaired secretion of immunoglobulins; the numbers of circulating B cells is usually in the normal range, but can be low. -
配列類似性
Contains 2 Ig-like C2-type (immunoglobulin-like) domains. -
翻訳後修飾
Phosphorylated on serine and threonine upon DNA damage, probably by ATM or ATR. Phosphorylated on tyrosine following B-cell activation. -
細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 12478 Mouse
- SwissProt: P25918 Mouse
- Unigene: 4360 Mouse
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別名
- Antibody deficiency due to defect in CD19 antibody
- Antibody deficiency due to defect in CD19, included antibody
- AW495831 antibody
see all
画像
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C57BL6 mouse splenocytes were incubated with Mouse IgA kappa monoclonal [S107] (ab37322) (left) or anti-CD19 [MB19-1] antibody (ab25177) (right) at 1ug/ml for 30 min at 4+°C. The secondary antibody Goat Anti-Mouse IgA alpha chain (FITC) was used at 1/200 dilution for 30 min at 4+°C. The cells were simultaneously stained with anti-CD19 APC (10ug/ml).
Acquisition of >30,000 total events were collected. Gating strategy - live lymphocytes
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (4)
ab25177 は 4 報の論文で使用されています。
- Sato S et al. Regulation of B lymphocyte development and activation by the CD19/CD21/CD81/Leu 13 complex requires the cytoplasmic domain of CD19. J Immunol 159:3278-87 (1997). PubMed: 9317126
- Sato S et al. CD19 and CD22 expression reciprocally regulates tyrosine phosphorylation of Vav protein during B lymphocyte signaling. Proc Natl Acad Sci U S A 94:13158-62 (1997). PubMed: 9371816
- Inaoki M et al. CD19-regulated signaling thresholds control peripheral tolerance and autoantibody production in B lymphocytes. J Exp Med 186:1923-31 (1997). PubMed: 9382890
- Sato S et al. CD22 is both a positive and negative regulator of B lymphocyte antigen receptor signal transduction: altered signaling in CD22-deficient mice. Immunity 5:551-62 (1996). PubMed: 8986715