製品の概要

  • 製品名
    Anti-CD19 antibody [CB19]
    CD19 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [CB19] to CD19
  • 由来種
    Mouse
  • アプリケーション
    適用あり: Flow Cytmore details
  • 種交差性
    交差種: Human

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab1255 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt
  • 追加情報
    Characterization of leukemias and lymphomas in human lysed whole peripheral blood or mononuclear cells separated by density gradient.

    CD19 immunofluorescence analysis can be performed on a flow cytometerequipped with an excitation source of 488nm and fitted with logarithmic amplifiers.

    10µl ofCD19 is sufficient for labelling of 1x106 cells.

    • ターゲット情報

    • 機能
      Assembles with the antigen receptor of B lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation.
    • 関連疾患
      Defects in CD19 are the cause of immunodeficiency common variable type 3 (CVID3) [MIM:613493]; also called antibody deficiency due to CD19 defect. CVID3 is a primary immunodeficiency characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections and an inability to mount an antibody response to antigen. The defect results from a failure of B-cell differentiation and impaired secretion of immunoglobulins; the numbers of circulating B cells is usually in the normal range, but can be low.
    • 配列類似性
      Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
    • 翻訳後修飾
      Phosphorylated on serine and threonine upon DNA damage, probably by ATM or ATR. Phosphorylated on tyrosine following B-cell activation.
    • 細胞内局在
      Membrane.
    • Information by UniProt
    • 参照データベース
      • 別名
        • Antibody deficiency due to defect in CD19, included antibody
        • AW495831 antibody
        • B lymphocyte antigen CD19 antibody
        • B lymphocyte surface antigen B4 antibody
        • B-lymphocyte antigen CD19 antibody
        • B-lymphocyte surface antigen B4 antibody
        • B4 antibody
        • CD19 antibody
        • CD19 antigen antibody
        • CD19 molecule antibody
        • Cd19 protein antibody
        • CD19_HUMAN antibody
        • CVID3 antibody
        • Differentiation antigen CD19 antibody
        • Leu 12 antibody
        • Leu-12 antibody
        • Leu12 antibody
        • MGC109570 antibody
        • MGC12802 antibody
        • T-cell surface antigen Leu-12 antibody
        see all

      プロトコール

      参考文献

      This product has been referenced in:
      • Bradbury LE  et al. The CD19/CD21 signal transducing complex of human B lymphocytes includes the target of antiproliferative antibody-1 and Leu-13 molecules. J Immunol 149:2841-50 (1992). Read more (PubMed: 1383329) »
      • Carter RH & Fearon DT CD19: lowering the threshold for antigen receptor stimulation of B lymphocytes. Science 256:105-7 (1992). Read more (PubMed: 1373518) »

      See all 2 Publications for this product

      ab1255 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

      レビューと Q&A

      レビューを送る 質問を送る

      1 Review or Q&A

      10
      Votes: Highest First

      Does the Ab bind its target CD19 if the cells are fixed (for example with formaldehyde) prior to staining?


      I have had confirmation from the lab that cells may be fixed prior CD19 staining and acquisition.

      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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