Anti-CD19 抗体 [CB19] (ab1255)

Submit a review Q&A (1)References (4)

Key features and details

  • Mouse monoclonal [CB19] to CD19
  • Reacts with: Human
  • Isotype: IgG1

製品の概要

  • 製品名

    Anti-CD19 antibody [CB19]
    CD19 一次抗体 製品一覧

  • 製品の詳細

    Mouse monoclonal [CB19] to CD19

  • 由来種

    Mouse

  • 種交差性

    交差種: Human

  • 特記事項

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

製品の特性

アプリケーション

  • 追加情報
    Characterization of leukemias and lymphomas in human lysed whole peripheral blood or mononuclear cells separated by density gradient.

    CD19 immunofluorescence analysis can be performed on a flow cytometerequipped with an excitation source of 488nm and fitted with logarithmic amplifiers.

    10µl ofCD19 is sufficient for labelling of 1x106 cells.

    • ターゲット情報

    • 機能

      Assembles with the antigen receptor of B lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation.

    • 関連疾患

      Defects in CD19 are the cause of immunodeficiency common variable type 3 (CVID3) [MIM:613493]; also called antibody deficiency due to CD19 defect. CVID3 is a primary immunodeficiency characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections and an inability to mount an antibody response to antigen. The defect results from a failure of B-cell differentiation and impaired secretion of immunoglobulins; the numbers of circulating B cells is usually in the normal range, but can be low.

    • 配列類似性

      Contains 2 Ig-like C2-type (immunoglobulin-like) domains.

    • 翻訳後修飾

      Phosphorylated on serine and threonine upon DNA damage, probably by ATM or ATR. Phosphorylated on tyrosine following B-cell activation.

    • 細胞内局在

      Membrane.
    • Information by UniProt

    • 参照データベース

      • 別名

        • Antibody deficiency due to defect in CD19 antibody
        • Antibody deficiency due to defect in CD19, included antibody
        • AW495831 antibody
        • B lymphocyte antigen CD19 antibody
        • B lymphocyte surface antigen B4 antibody
        • B-lymphocyte antigen CD19 antibody
        • B-lymphocyte surface antigen B4 antibody
        • B4 antibody
        • CD19 antibody
        • CD19 antigen antibody
        • CD19 molecule antibody
        • Cd19 protein antibody
        • CD19_HUMAN antibody
        • CVID3 antibody
        • Differentiation antigen CD19 antibody
        • Leu 12 antibody
        • Leu-12 antibody
        • Leu12 antibody
        • MGC109570 antibody
        • MGC12802 antibody
        • T-cell surface antigen Leu-12 antibody
        see all

      参考文献 (4)

      ab1255 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

      ab1255 は 4 報の論文で使用されています。

      • Ruella M  et al. Induction of resistance to chimeric antigen receptor T cell therapy by transduction of a single leukemic B cell. Nat Med 24:1499-1503 (2018). PubMed: 30275568
      • Silveira ELV  et al. Immune Cell Dynamics in Rhesus Macaques Infected with a Brazilian Strain of Zika Virus. J Immunol 199:1003-1011 (2017). PubMed: 28667164
      • Bradbury LE  et al. The CD19/CD21 signal transducing complex of human B lymphocytes includes the target of antiproliferative antibody-1 and Leu-13 molecules. J Immunol 149:2841-50 (1992). PubMed: 1383329
      • Carter RH & Fearon DT CD19: lowering the threshold for antigen receptor stimulation of B lymphocytes. Science 256:105-7 (1992). PubMed: 1373518

      レビューと Q&A

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      レビューを送る 質問を送る

      Question

      Does the Ab bind its target CD19 if the cells are fixed (for example with formaldehyde) prior to staining?


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      Answer

      I have had confirmation from the lab that cells may be fixed prior CD19 staining and acquisition.

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