Anti-CD19 抗体 [CB19] (ab1255)

Submit a review Q&A (1)References (3)

Key features and details

  • Mouse monoclonal [CB19] to CD19
  • Suitable for: Flow Cyt
  • Reacts with: Human
  • Isotype: IgG1

製品の概要

  • 製品名

    Anti-CD19 antibody [CB19]
    CD19 一次抗体 製品一覧

  • 製品の詳細

    Mouse monoclonal [CB19] to CD19

  • 由来種

    Mouse

  • アプリケーション

    適用あり: Flow Cytmore details

  • 種交差性

    交差種: Human

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab1255 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt
  • 追加情報
    Characterization of leukemias and lymphomas in human lysed whole peripheral blood or mononuclear cells separated by density gradient.

    CD19 immunofluorescence analysis can be performed on a flow cytometerequipped with an excitation source of 488nm and fitted with logarithmic amplifiers.

    10µl ofCD19 is sufficient for labelling of 1x106 cells.

    • ターゲット情報

    • 機能

      Assembles with the antigen receptor of B lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation.

    • 関連疾患

      Defects in CD19 are the cause of immunodeficiency common variable type 3 (CVID3) [MIM:613493]; also called antibody deficiency due to CD19 defect. CVID3 is a primary immunodeficiency characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections and an inability to mount an antibody response to antigen. The defect results from a failure of B-cell differentiation and impaired secretion of immunoglobulins; the numbers of circulating B cells is usually in the normal range, but can be low.

    • 配列類似性

      Contains 2 Ig-like C2-type (immunoglobulin-like) domains.

    • 翻訳後修飾

      Phosphorylated on serine and threonine upon DNA damage, probably by ATM or ATR. Phosphorylated on tyrosine following B-cell activation.

    • 細胞内局在

      Membrane.
    • Information by UniProt

    • 参照データベース

      • 別名

        • Antibody deficiency due to defect in CD19 antibody
        • Antibody deficiency due to defect in CD19, included antibody
        • AW495831 antibody
        • B lymphocyte antigen CD19 antibody
        • B lymphocyte surface antigen B4 antibody
        • B-lymphocyte antigen CD19 antibody
        • B-lymphocyte surface antigen B4 antibody
        • B4 antibody
        • CD19 antibody
        • CD19 antigen antibody
        • CD19 molecule antibody
        • Cd19 protein antibody
        • CD19_HUMAN antibody
        • CVID3 antibody
        • Differentiation antigen CD19 antibody
        • Leu 12 antibody
        • Leu-12 antibody
        • Leu12 antibody
        • MGC109570 antibody
        • MGC12802 antibody
        • T-cell surface antigen Leu-12 antibody
        see all

      参考文献

      ab1255 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

      ab1255 は 3 報の論文で使用されています。

      • Ruella M  et al. Induction of resistance to chimeric antigen receptor T cell therapy by transduction of a single leukemic B cell. Nat Med 24:1499-1503 (2018). PubMed: 30275568
      • Bradbury LE  et al. The CD19/CD21 signal transducing complex of human B lymphocytes includes the target of antiproliferative antibody-1 and Leu-13 molecules. J Immunol 149:2841-50 (1992). PubMed: 1383329
      • Carter RH & Fearon DT CD19: lowering the threshold for antigen receptor stimulation of B lymphocytes. Science 256:105-7 (1992). PubMed: 1373518

      レビューと Q&A

      Show All レビュー Q&A
      レビューを送る 質問を送る

      Question

      Does the Ab bind its target CD19 if the cells are fixed (for example with formaldehyde) prior to staining?


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      Answer

      I have had confirmation from the lab that cells may be fixed prior CD19 staining and acquisition.

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