Anti-CD18 抗体 [MEM-48] (ab657)
![Flow Cytometry - Anti-CD18 antibody [MEM-48] (ab657)](https://www.abcam.co.jp/ps/products/0/ab657/Images/ab657-199407-anti-cd18-antibody-mem-48-flow-cytometry.jpg "Flow Cytometry - Anti-CD18 antibody [MEM-48] (ab657)")
Key features and details
- Mouse monoclonal [MEM-48] to CD18
- Suitable for: Flow Cyt
- Reacts with: Human
- Isotype: IgG1
製品の概要
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製品名
Anti-CD18 antibody [MEM-48]
CD18 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [MEM-48] to CD18 -
由来種
Mouse -
特異性
Human CD18 antigen. This antibody recognizes all three leucocyte integrins. -
アプリケーション
適用あり: Flow Cytmore details -
種交差性
交差種: Human -
免疫原
Tissue/ cell preparation (Human). (Leucocytes of a patient suffering from a LGL-type leukaemia).
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特記事項
This product was changed from ascites to tissue culture supernatant on 24th January 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: PBS -
Concentration information loading... -
精製度
Protein A purified -
特記事項（精製）
Purified from TCS. Purity >95% by SDS-PAGE. -
ポリ/モノ
モノクローナル -
クローン名
MEM-48 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab657の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Flow Cyt | (1) |
Use 0.1-1µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
| 特記事項 |
|---|
|
Flow Cyt
Use 0.1-1µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
ターゲット情報
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機能
Integrin alpha-L/beta-2 is a receptor for ICAM1, ICAM2, ICAM3 and ICAM4. Integrins alpha-M/beta-2 and alpha-X/beta-2 are receptors for the iC3b fragment of the third complement component and for fibrinogen. Integrin alpha-X/beta-2 recognizes the sequence G-P-R in fibrinogen alpha-chain. Integrin alpha-M/beta-2 recognizes P1 and P2 peptides of fibrinogen gamma chain. Integrin alpha-M/beta-2 is also a receptor for factor X. Integrin alpha-D/beta-2 is a receptor for ICAM3 and VCAM1. -
関連疾患
Defects in ITGB2 are the cause of leukocyte adhesion deficiency type 1 (LAD1) [MIM:116920]. LAD1 patients have recurrent bacterial infections and their leukocytes are deficient in a wide range of adhesion-dependent functions. -
配列類似性
Belongs to the integrin beta chain family.
Contains 1 VWFA domain. -
翻訳後修飾
Both Ser-745 and Ser-756 become phosphorylated when T-cells are exposed to phorbol esters. Phosphorylation on Thr-758 (but not on Ser-756) allows interaction with 14-3-3 proteins. -
細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 3689 Human
- Omim: 600065 Human
- SwissProt: P05107 Human
- Unigene: 375957 Human
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別名
- 95 subunit beta antibody
- Beta 2 (antigen CD18 (p95) antibody
- CD 18 antibody
see all
画像
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Flow Cytometry - Anti-CD18 antibody [MEM-48] (ab657)
Human peripheral blood lymphocytes stained with ab657 (red line). Human whole blood was processed using a modified protocol based on Chow et al, 2005 (PMID: 16080188). In brief, human whole blood was fixed in 4% formaldehyde (methanol-free) for 10 min at 22ºC. Red blood cells were then lyzed by the addition of Triton X-100 (final concentration - 0.1%) for 15 min at 37ºC. For experimentation, cells were treated with 50% methanol (-20°C) for 15 min at 4ºC. Cells were then incubated with the antibody (ab657, 1μg/1x106 cells) for 30 min at 4ºC. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 4ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >30,000 total events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. Gating strategy - peripheral blood lymphocytes.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (8)
ab657 は 8 報の論文で使用されています。
- Nice JB et al. Aggregatibacter actinomycetemcomitans Leukotoxin Is Delivered to Host Cells in an LFA-1-Indepdendent Manner When Associated with Outer Membrane Vesicles. Toxins (Basel) 10:N/A (2018). PubMed: 30322160
- Laufer JM et al. CCR7 Is Recruited to the Immunological Synapse, Acts as Co-stimulatory Molecule and Drives LFA-1 Clustering for Efficient T Cell Adhesion Through ZAP70. Front Immunol 9:3115 (2018). PubMed: 30692994
- Asadi A et al. Characterization of Antibodies to Products of Proinsulin Processing Using Immunofluorescence Staining of Pancreas in Multiple Species. J Histochem Cytochem 63:646-62 (2015). PubMed: 26216140
- Xiang Y et al. Analysis on the relevance of asthma susceptibility with the alteration of integrin ß 4 expression. PLoS One 9:e95533 (2014). WB ; Human . PubMed: 24740264
- Giese S & Marsh M Tetherin can restrict cell-free and cell-cell transmission of HIV from primary macrophages to T cells. PLoS Pathog 10:e1004189 (2014). ICC/IF . PubMed: 24991932
- Evani SJ et al. Monocytes mediate metastatic breast tumor cell adhesion to endothelium under flow. FASEB J 27:3017-29 (2013). Human . PubMed: 23616566
- Drbal K et al. Human leukocytes contain a large pool of free forms of CD18. Biochem Biophys Res Commun 275:295-9 (2000). PubMed: 10964661
- Bazil V et al. Monoclonal antibodies against human leucocyte antigens. IV. Antibodies against subunits of the LFA-1 (CD11a/CD18) leucocyte-adhesion glycoprotein. Folia Biol (Praha) 36:41-50 (1990). PubMed: 1971601