Anti-CD18 抗体 [MEM-48] (ab657)

Reviews (1)Q&A (1)References (8)

Key features and details

  • Mouse monoclonal [MEM-48] to CD18
  • Suitable for: IHC-P, Flow Cyt, IHC-Fr, IP, WB, Functional Studies
  • Reacts with: Human
  • Isotype: IgG1

製品の概要

  • 製品名

    Anti-CD18 antibody [MEM-48]
    CD18 一次抗体 製品一覧

  • 製品の詳細

    Mouse monoclonal [MEM-48] to CD18

  • 由来種

    Mouse

  • 特異性

    Human CD18 antigen. This antibody recognizes all three leucocyte integrins.

  • アプリケーション

    適用あり: IHC-P, Flow Cyt, IHC-Fr, IP, WB, Functional Studiesmore details

  • 種交差性

    交差種: Human

  • 免疫原

    Tissue/ cell preparation (Human). (Leucocytes of a patient suffering from a LGL-type leukaemia).

  • 特記事項

    This product was changed from ascites to tissue culture supernatant on 24th January 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab657 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use at an assay dependent concentration.
Flow Cyt Use 0.1-1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
IHC-Fr Use a concentration of 10 µg/ml.
IP Use at an assay dependent concentration.
WB Use a concentration of 2 µg/ml. Use under non reducing condition.

BSA is recommended for blocking.
Functional Studies Use at an assay dependent concentration. The antibody induces high-affinity conformation of LFA-1 complex.

ターゲット情報

  • 機能

    Integrin alpha-L/beta-2 is a receptor for ICAM1, ICAM2, ICAM3 and ICAM4. Integrins alpha-M/beta-2 and alpha-X/beta-2 are receptors for the iC3b fragment of the third complement component and for fibrinogen. Integrin alpha-X/beta-2 recognizes the sequence G-P-R in fibrinogen alpha-chain. Integrin alpha-M/beta-2 recognizes P1 and P2 peptides of fibrinogen gamma chain. Integrin alpha-M/beta-2 is also a receptor for factor X. Integrin alpha-D/beta-2 is a receptor for ICAM3 and VCAM1.

  • 関連疾患

    Defects in ITGB2 are the cause of leukocyte adhesion deficiency type 1 (LAD1) [MIM:116920]. LAD1 patients have recurrent bacterial infections and their leukocytes are deficient in a wide range of adhesion-dependent functions.

  • 配列類似性

    Belongs to the integrin beta chain family.
    Contains 1 VWFA domain.

  • 翻訳後修飾

    Both Ser-745 and Ser-756 become phosphorylated when T-cells are exposed to phorbol esters. Phosphorylation on Thr-758 (but not on Ser-756) allows interaction with 14-3-3 proteins.

  • 細胞内局在

    Membrane.
  • Information by UniProt

  • 参照データベース

    • 別名

      • 95 subunit beta antibody
      • Beta 2 (antigen CD18 (p95) antibody
      • CD 18 antibody
      • CD18 antibody
      • Cell surface adhesion glycoprotein LFA 1/CR3/P150,959 beta subunit precursor) antibody
      • Cell surface adhesion glycoproteins LFA 1/CR3/p150,95 subunit beta antibody
      • Cell surface adhesion glycoproteins LFA-1/CR3/p150 antibody
      • Complement component 3 receptor 3 and 4 subunit antibody
      • Complement receptor C3 beta subunit antibody
      • Complement receptor C3 subunit beta antibody
      • Integrin beta 2 antibody
      • Integrin beta chain beta 2 antibody
      • Integrin beta-2 (ITGB2) antibody
      • Integrin beta-2 antibody
      • Integrin, beta 2 (complement component 3 receptor 3 and 4 subunit) antibody
      • ITB2_HUMAN antibody
      • ITGB2 antibody
      • LAD antibody
      • LCAMB antibody
      • Leukocyte associated antigens CD18/11A, CD18/11B, CD18/11C antibody
      • Leukocyte cell adhesion molecule CD18 antibody
      • LFA 1 antibody
      • LFA1 antibody
      • Lymphocyte function associated antigen 1 (LFA1) antibody
      • Lymphocyte function associated antigen 1 antibody
      • MAC 1 antibody
      • MAC1 antibody
      • Macrophage antigen 1 (mac1) beta antibody
      • MF17 antibody
      • MFI7 antibody
      • OTTHUMP00000115278 antibody
      • OTTHUMP00000115279 antibody
      • OTTHUMP00000115280 antibody
      • OTTHUMP00000115281 antibody
      • OTTHUMP00000115282 antibody
      see all

    画像

    • Flow Cytometry - Anti-CD18 antibody [MEM-48] (ab657)
      Flow Cytometry - Anti-CD18 antibody [MEM-48] (ab657)

      Human peripheral blood lymphocytes stained with ab657 (red line). Human whole blood was processed using a modified protocol based on Chow et al, 2005 (PMID: 16080188). In brief, human whole blood was fixed in 4% formaldehyde (methanol-free) for 10 min at 22ºC. Red blood cells were then lyzed by the addition of Triton X-100 (final concentration - 0.1%) for 15 min at 37ºC. For experimentation, cells were treated with 50% methanol (-20°C) for 15 min at 4ºC. Cells were then incubated with the antibody (ab657, 1μg/1x106 cells) for 30 min at 4ºC. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 4ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >30,000 total events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. Gating strategy - peripheral blood lymphocytes.

    参考文献

    ab657 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab657 は 8 報の論文で使用されています。

    • Nice JB  et al. Aggregatibacter actinomycetemcomitans Leukotoxin Is Delivered to Host Cells in an LFA-1-Indepdendent Manner When Associated with Outer Membrane Vesicles. Toxins (Basel) 10:N/A (2018). PubMed: 30322160
    • Laufer JM  et al. CCR7 Is Recruited to the Immunological Synapse, Acts as Co-stimulatory Molecule and Drives LFA-1 Clustering for Efficient T Cell Adhesion Through ZAP70. Front Immunol 9:3115 (2018). PubMed: 30692994
    • Asadi A  et al. Characterization of Antibodies to Products of Proinsulin Processing Using Immunofluorescence Staining of Pancreas in Multiple Species. J Histochem Cytochem 63:646-62 (2015). PubMed: 26216140
    • Xiang Y  et al. Analysis on the relevance of asthma susceptibility with the alteration of integrin ß 4 expression. PLoS One 9:e95533 (2014). WB ; Human . PubMed: 24740264
    • Giese S & Marsh M Tetherin can restrict cell-free and cell-cell transmission of HIV from primary macrophages to T cells. PLoS Pathog 10:e1004189 (2014). ICC/IF . PubMed: 24991932
    • Evani SJ  et al. Monocytes mediate metastatic breast tumor cell adhesion to endothelium under flow. FASEB J 27:3017-29 (2013). Human . PubMed: 23616566
    • Drbal K  et al. Human leukocytes contain a large pool of free forms of CD18. Biochem Biophys Res Commun 275:295-9 (2000). PubMed: 10964661
    • Bazil V  et al. Monoclonal antibodies against human leucocyte antigens. IV. Antibodies against subunits of the LFA-1 (CD11a/CD18) leucocyte-adhesion glycoprotein. Folia Biol (Praha) 36:41-50 (1990). PubMed: 1971601

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    Flow Cytometry abreview for Anti-CD18 antibody [MEM-48]

     Good
    Abreviews
    Application
    Flow Cytometry
    Sample
    Human Cell lysate - whole cell (HEK293 cells overexpressing cd18/cd11b)
    Specification
    HEK293 cells overexpressing cd18/cd11b
    Permeabilization
    No
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    Abcam user community

    Verified customer

    投稿 Aug 18 2005

    Question

    When these antibodies were tested in Western blotting, what cell line was used for characterization?

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    Answer

    Thank you for your enquiry. These two antibodies were characterized using HPB-ALL or Jurkat T cells. Triplet of zones on NON-REDUCING lysates is usually observed (78-96 kDa). If you have any additional questins, please contact us again.

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