Anti-CD18 抗体 [C71/16] - BSA and Azide free (ab272444)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rat monoclonal [C71/16] to CD18 - BSA and Azide free
- Suitable for: Flow Cyt
- Reacts with: Mouse
製品の概要
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製品名
Anti-CD18 antibody [C71/16] - BSA and Azide free
CD18 一次抗体 製品一覧 -
製品の詳細
Rat monoclonal [C71/16] to CD18 - BSA and Azide free -
由来種
Rat -
アプリケーション
適用あり: Flow Cytmore details -
種交差性
交差種: Mouse -
免疫原
Tissue, cells or virus. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- Flow Cyt: Mouse peripheral blood mononuclear cell (PBMC).
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特記事項
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
ab272444 is the carrier-free version of ab19580. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
バッファー
pH: 7.2
Constituent: PBS -
キャリア・フリー
はい -
Concentration information loading... -
精製度
Ion Exchange Chromatography -
ポリ/モノ
モノクローナル -
クローン名
C71/16 -
アイソタイプ
IgG2a -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
アプリケーション
Our Abpromise guarantee covers the use of ab272444 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Flow Cyt | Use at an assay dependent concentration. |
ターゲット情報
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機能
Integrin alpha-L/beta-2 is a receptor for ICAM1, ICAM2, ICAM3 and ICAM4. Integrins alpha-M/beta-2 and alpha-X/beta-2 are receptors for the iC3b fragment of the third complement component and for fibrinogen. Integrin alpha-X/beta-2 recognizes the sequence G-P-R in fibrinogen alpha-chain. Integrin alpha-M/beta-2 recognizes P1 and P2 peptides of fibrinogen gamma chain. Integrin alpha-M/beta-2 is also a receptor for factor X. Integrin alpha-D/beta-2 is a receptor for ICAM3 and VCAM1. -
関連疾患
Defects in ITGB2 are the cause of leukocyte adhesion deficiency type 1 (LAD1) [MIM:116920]. LAD1 patients have recurrent bacterial infections and their leukocytes are deficient in a wide range of adhesion-dependent functions. -
配列類似性
Belongs to the integrin beta chain family.
Contains 1 VWFA domain. -
翻訳後修飾
Both Ser-745 and Ser-756 become phosphorylated when T-cells are exposed to phorbol esters. Phosphorylation on Thr-758 (but not on Ser-756) allows interaction with 14-3-3 proteins. -
細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 16414 Mouse
- SwissProt: P11835 Mouse
- Unigene: 1137 Mouse
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別名
- 95 subunit beta antibody
- Beta 2 (antigen CD18 (p95) antibody
- CD 18 antibody
see all
画像
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Flow Cytometry - (ab272444)
Flow cytometric analysis of mouse peripheral blood mononuclear cell (PBMC) cells labelling CD18 with ab19580 at 1/1000 dilution (1.003µg/ml) (Right panel) compared with a rat monoclonal IgG isotype control (Left panel). Goat F(ab)2 Anti-rat IgG Fc (Alexa Fluor® 488, ab150161) at 1/2000 dilution was used as the secondary antibody. Gated on viable cells.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab19580).
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Flow Cytometry - (ab272444)Flow cytometric analysis of mouse peripheral blood mononuclear cell (PBMC) cells labelling CD18 with ab19580 at 1/1000 dilution (1.003µg/ml) (Red) compared with a rat monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat F(ab)2 Anti-rat IgG Fc (Alexa Fluor® 488, ab150161) at 1/2000 dilution was used as the secondary antibody. Gated on viable cells.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab19580).
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![Anti-CD18 antibody [C71/16] - BSA and Azide free (ab272444)](https://www.abcam.co.jp/ps/products/272/ab272444/Images/ab272444-3-benefits-of-recombinant-antibodies.png)
Anti-CD18 antibody [C71/16] - BSA and Azide free (ab272444)
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
参考文献 (0)
ab272444 は論文での使用が確認できていません。
