Anti-CD168 抗体 [EPR4055] (ab108339)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4055] to CD168
- Suitable for: WB, IP, IHC-P
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-CD168 antibody [EPR4055]
CD168 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR4055] to CD168 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IP, IHC-Pmore details
適用なし: ICC/IF -
種交差性
交差種: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- T47-D MCF-7, SKBR-3 and LnCaP cell lysate Paraffin-embedded human breast carcinoma tissue Paraffin-embedded human testis tissue IP: T-47D whole cell lysate.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR4055 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab108339の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 84 kDa.
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IP |
1/10 - 1/100.
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IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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特記事項 |
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WB
1/1000 - 1/10000. Predicted molecular weight: 84 kDa. |
IP
1/10 - 1/100. |
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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機能
Involved in cell motility. When hyaluronan binds to HMMR, the phosphorylation of a number of proteins, including the focal adhesion kinase occurs. May also be involved in cellular transformation and metastasis formation, and in regulating extracellular-regulated kinase (ERK) activity. -
組織特異性
Expressed in breast cancer cell lines and in normal breast tissue. -
細胞内局在
Cell surface. Cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 3161 Human
- Omim: 600936 Human
- SwissProt: O75330 Human
- Unigene: 728200 Human
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別名
- CD168 antibody
- CD168 antigen antibody
- HMMR antibody
see all
画像
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Lane 1: Wild type HAP1 whole cell lysate (20 µg)
Lane 2: Empty knockout HAP1 whole cell lysate (20 µg)
Lane 3: CD168 whole cell lysate (20 µg)Lanes 1 - 3: Merged signal (red and green). Green - ab108339 observed at 90 kDa. Red - loading control, ab9484, observed at 37 kDa.
ab108339 was shown to specifically react with CD168 when CD168 knockout samples were used. Wild-type and Empty knockout samples were subjected to SDS-PAGE. Ab108339 and ab9484 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
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ab108339, at 1/100, staining CD168 in paraffin-embedded human breast carcinoma tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Purified ab108339 at 1/50 dilution (2µg) immunoprecipitating CD168 in T-47D whole cell lysate.
Lane 1 (input): T-47D (Human ductal breast epithelial tumor epithelial cell) whole cell lysate 10µg
Lane 2 (+): ab108339 + T-47D whole cell lysate.
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab108339 in T-47D whole cell lysate.
VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
Blocking Buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
Observed band size: 85 kDa -
All lanes : Anti-CD168 antibody [EPR4055] (ab108339) at 1/1000 dilution
Lane 1 : T47-D cell lysate
Lane 2 : MCF-7 cell lysate
Lane 3 : SKBR-3 cell lysate
Lane 4 : LnCaP cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 84 kDa -
ab108339, at 1/100, staining CD168 in paraffin-embedded human testis tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ab108339 showing positive staining in Normal thymus tissue.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ab108339 showing positive staining in Normal stomach tissue.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ab108339 showing negative staining in Normal brain tissue.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ab108339 showing positive staining in Colonic adenocarcinoma tissue.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ab108339 showing negative staining in Normal liver tissue.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ab108339 showing positive staining in Normal tonsil tissue.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (7)
ab108339 は 7 報の論文で使用されています。
- Burren S et al. RHAMM in liver metastases of stage IV colorectal cancer with mismatch-repair proficient status correlates with tumor budding, cytotoxic T-cells and PD-1/PD-L1. Pathol Res Pract 223:153486 (2021). PubMed: 34051513
- Chen X et al. RHAMMB-mediated bifunctional nanotherapy targeting Bcl-xL and mitochondria for pancreatic neuroendocrine tumor treatment. Mol Ther Oncolytics 23:277-287 (2021). PubMed: 34761107
- Gao C et al. Effect of receptor for hyaluronan-mediated motility inhibition on radiosensitivity of lung adenocarcinoma A549 cells. Transl Cancer Res 8:410-421 (2019). PubMed: 35116773
- Chen YT et al. Immunohistochemical analysis of RHAMM expression in normal and neoplastic human tissues: a cell cycle protein with distinctive expression in mitotic cells and testicular germ cells. Oncotarget 9:20941-20952 (2018). PubMed: 29765511
- Wang K & Zhang T Prognostic significance of CD168 overexpression in colorectal cancer. Oncol Lett 12:2555-2559 (2016). IHC ; Human . PubMed: 27698827
- Wang D et al. Expression of the receptor for hyaluronic acid mediated motility (RHAMM) is associated with poor prognosis and metastasis in non-small cell lung carcinoma. Oncotarget 7:39957-39969 (2016). WB, IHC . PubMed: 27220886
- Picaud L et al. Evaluation of the effects of hyaluronic acid-carboxymethyl cellulose barrier on ovarian tumor progression. J Ovarian Res 7:40 (2014). WB ; Human . PubMed: 24739440