Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (metastatic melanoma)
Permeabilization
No
Specification
metastatic melanoma
Blocking step
(agent) for 10 minute(s) · Concentration: 0.25% · Temperature: RT°C
Fixative
Acetone
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Diluent: 1% FBS in TBS
Dilution
1/50
Secondary antibody
Dilution
1/200
Name
Non-Abcam antibody was used: Alexa 647 anti-rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 647
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 647
Additional data
Abcam response
Thank you for your valuable feedback and for participating in our Abtrial testing program. We are sorry to hear that this antibody has not given you good results in IHC-Fr using human sample. This antibody has not been tested with this IHC-Fr before, and it is possible that it may not be suitable for use.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Sep 11 2015