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RabMAb

Anti-CD163 抗体 [EPR14643-36] - C-terminal (ab189915)

製品の概要

  • 製品名
    Anti-CD163 antibody [EPR14643-36] - C-terminal
    CD163 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR14643-36] to CD163 - C-terminal
  • 由来種
    Rabbit
  • アプリケーション
    適用あり: IP, WB, IHC-Pmore details
    適用なし: Flow Cyt
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant fragment within Human CD163 aa 1000 to the C-terminus (C terminal). The exact sequence is proprietary.

  • ポジティブ・コントロール
    • Human placenta and Human fetal liver lysates; Human liver and tonsil tissue.
  • 特記事項

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab189915 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IP 1/30 - 1/40.
WB 1/1000 - 1/10000.
IHC-P 1/500 - 1/1000.
  • 追加情報
    Is unsuitable for Flow Cyt.
  • ターゲット情報

    • 機能
      Acute phase-regulated receptor involved in clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages and may thereby protect tissues from free hemoglobin-mediated oxidative damage. May play a role in the uptake and recycling of iron, via endocytosis of hemoglobin/haptoglobin and subsequent breakdown of heme. Binds hemoglobin/haptoglobin complexes in a calcium-dependent and pH-dependent manner. Exhibits a higher affinity for complexes of hemoglobin and multimeric haptoglobin of HP*1F phenotype than for complexes of hemoglobin and dimeric haptoglobin of HP*1S phenotype. Induces a cascade of intracellular signals that involves tyrosine kinase-dependent calcium mobilization, inositol triphosphate production and secretion of IL6 and CSF1. Isoform 3 exhibits the higher capacity for ligand endocytosis and the more pronounced surface expression when expressed in cells.
      After shedding, the soluble form (sCD163) may play an anti-inflammatory role, and may be a valuable diagnostic parameter for monitoring macrophage activation in inflammatory conditions.
    • 組織特異性
      Expressed in monocytes and mature macrophages such as Kupffer cells in the liver, red pulp macrophages in the spleen, cortical macrophages in the thymus, resident bone marrow macrophages and meningeal macrophages of the central nervous system. Expressed also in blood. Isoform 1 is the lowest abundant in the blood. Isoform 2 is the lowest abundant in the liver and the spleen. Isoform 3 is the predominant isoform detected in the blood.
    • 配列類似性
      Contains 9 SRCR domains.
    • ドメイン
      The SRCR domain 3 mediates calcium-sensitive interaction with hemoglobin/haptoglobin complexes.
    • 翻訳後修飾
      A soluble form (sCD163) is produced by proteolytic shedding which can be induced by lipopolysaccharide, phorbol ester and Fc region of immunoglobulin gamma. This cleavage is dependent on protein kinase C and tyrosine kinases and can be blocked by protease inhibitors. The shedding is inhibited by the tissue inhibitor of metalloproteinase TIMP3, and thus probably induced by membrane-bound metalloproteinases ADAMs.
      Phosphorylated.
    • 細胞内局在
      Secreted and Cell membrane. Isoform 1 and isoform 2 show a lower surface expression when expressed in cells.
    • Information by UniProt
    • 参照データベース
    • 別名
      • C163A_HUMAN antibody
      • CD 163 antibody
      • CD163 antibody
      • CD163 antigen antibody
      • CD163 molecule antibody
      • Hemoglobin scavenger receptor antibody
      • M130 antibody
      • M130 antigen precursor antibody
      • Macrophage associated antigen antibody
      • MM130 antibody
      • OTTHUMP00000238617 antibody
      • OTTHUMP00000238618 antibody
      • OTTHUMP00000238619 antibody
      • OTTHUMP00000238620 antibody
      • SCARI1 antibody
      • Scavenger receptor cysteine rich type 1 protein M130 antibody
      • sCD163 antibody
      • Soluble CD163 antibody
      see all

    画像

    • Immunohistochemical analysis of paraffin embedded Human liver tissue labeling CD163 with ab189915 at 1/1000 with prediluted ImmunoHistoprobe(Ready to use) HRP Polymer for Rabbit IgG as secondary antibody.

    • Immunoprecipitation. ab189915 at 1/1000 labeling CD163 in Human fetal kidney lysate immunoprecipitated using ab189915 at 1/50.

    • Immunohistochemical analysis of paraffin embedded human liver tissue labeling CD163 with ab189915 at 1/100. No blocking step was performed.Anti-Rabbit HRP polymer was used as the secondary detection system. Heat-mediated antigen retrieval was performed using EDTA based pH 9.0 buffer.

    • Immunohistochemical analysis of paraffin embedded human tonsil tissue labeling CD163 with ab189915 at 1/100. No blocking step was performed. Anti-Rabbit HRP polymer was used as the secondary detection system. Heat-mediated antigen retrieval was performed using EDTA based pH 9.0 buffer.

    • All lanes : Anti-CD163 antibody [EPR14643-36] - C-terminal (ab189915) at 1/5000 dilution

      Lane 1 : Human placenta lysate
      Lane 2 : Human fetal liver lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    参考文献

    This product has been referenced in:
    • Tseng HY  et al. Oncogenic MCT-1 activation promotes YY1-EGFR-MnSOD signaling and tumor progression. Oncogenesis 6:e313 (2017). IHC ; Human . Read more (PubMed: 28394354) »
    • Cheng Z  et al. CD163 as a novel target gene of STAT3 is a potential therapeutic target for gastric cancer. Oncotarget 8:87244-87262 (2017). Read more (PubMed: 29152078) »
    See all 6 Publications for this product

    レビューと Q&A

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step
    BlockAid as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 20°C
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citric buffer, pH 6
    Sample
    Human Tissue sections (Skin)
    Specification
    Skin
    Permeabilization
    Yes - 0.2% Triton-X100
    Fixative
    Formaldehyde

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    投稿 Apr 06 2015

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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