Anti-CD163 抗体 [BLR087G] - BSA free (ab272066)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD163 antibody [BLR087G] - BSA free (ab272066)](https://www.abcam.co.jp/ps/products/272/ab272066/Images/ab272066-3-ab265592366263AntiCD163antibodyBLR087Gimmunohistochemistrylivercarcinomahuman.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD163 antibody [BLR087G] - BSA free (ab272066)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [BLR087G] to CD163 - BSA free
- Suitable for: IHC-P
- Reacts with: Human
製品の概要
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製品名
Anti-CD163 antibody [BLR087G] - BSA free
CD163 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [BLR087G] to CD163 - BSA free -
由来種
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P Human
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免疫原
Synthetic peptide within Human CD163 aa 850-900. The exact sequence is proprietary. NP_004235.4
Database link: Q86VB7 -
ポジティブ･コントロール
- IHC-P: Human liver carcinoma and lung carcinoma tissue. Human lung carcinoma pleural fluid.
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特記事項
This product is sold under License from Bethyl Laboratories, Inc
ab272066 is the carrier-free version of ab265592. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 8.20
Preservative: 0.09% Sodium azide
Constituent: 99% Borate buffered saline -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
Purified from cell culture supernatant. -
ポリ/モノ
モノクローナル -
クローン名
BLR087G -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Related Products
アプリケーション
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab272066 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| アプリケーション | Species |
|---|---|
| IHC-P |
Human
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| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| 特記事項 |
|---|
|
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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機能
Acute phase-regulated receptor involved in clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages and may thereby protect tissues from free hemoglobin-mediated oxidative damage. May play a role in the uptake and recycling of iron, via endocytosis of hemoglobin/haptoglobin and subsequent breakdown of heme. Binds hemoglobin/haptoglobin complexes in a calcium-dependent and pH-dependent manner. Exhibits a higher affinity for complexes of hemoglobin and multimeric haptoglobin of HP*1F phenotype than for complexes of hemoglobin and dimeric haptoglobin of HP*1S phenotype. Induces a cascade of intracellular signals that involves tyrosine kinase-dependent calcium mobilization, inositol triphosphate production and secretion of IL6 and CSF1. Isoform 3 exhibits the higher capacity for ligand endocytosis and the more pronounced surface expression when expressed in cells.
After shedding, the soluble form (sCD163) may play an anti-inflammatory role, and may be a valuable diagnostic parameter for monitoring macrophage activation in inflammatory conditions. -
組織特異性
Expressed in monocytes and mature macrophages such as Kupffer cells in the liver, red pulp macrophages in the spleen, cortical macrophages in the thymus, resident bone marrow macrophages and meningeal macrophages of the central nervous system. Expressed also in blood. Isoform 1 is the lowest abundant in the blood. Isoform 2 is the lowest abundant in the liver and the spleen. Isoform 3 is the predominant isoform detected in the blood. -
配列類似性
Contains 9 SRCR domains. -
ドメイン
The SRCR domain 3 mediates calcium-sensitive interaction with hemoglobin/haptoglobin complexes. -
翻訳後修飾
A soluble form (sCD163) is produced by proteolytic shedding which can be induced by lipopolysaccharide, phorbol ester and Fc region of immunoglobulin gamma. This cleavage is dependent on protein kinase C and tyrosine kinases and can be blocked by protease inhibitors. The shedding is inhibited by the tissue inhibitor of metalloproteinase TIMP3, and thus probably induced by membrane-bound metalloproteinases ADAMs.
Phosphorylated. -
細胞内局在
Secreted and Cell membrane. Isoform 1 and isoform 2 show a lower surface expression when expressed in cells. - Information by UniProt
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参照データベース
- Entrez Gene: 9332 Human
- Omim: 605545 Human
- SwissProt: Q86VB7 Human
- Unigene: 504641 Human
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別名
- C163A_HUMAN antibody
- CD 163 antibody
- CD163 antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD163 antibody [BLR087G] - BSA free (ab272066)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded human liver carcinoma tissue labeling CD163 with ab265592 at 1/100 dilution. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.
This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (ab265592).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD163 antibody [BLR087G] - BSA free (ab272066)](https://www.abcam.co.jp/ps/products/272/ab272066/Images/ab272066-2-ab265592366264AntiCD163antibodyBLR087Gimmunohistochemistrylungcarcinomahuman.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD163 antibody [BLR087G] - BSA free (ab272066)Immunohistochemical analysis of formalin-fixed, paraffin-embedded human lung carcinoma tissue labeling CD163 with ab265592 at 1/100 dilution. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.
This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (ab265592).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD163 antibody [BLR087G] - BSA free (ab272066)](https://www.abcam.co.jp/ps/products/272/ab272066/Images/ab272066-1-ab265592366265AntiCD163antibodyBLR087Gimmunohistochemistrylungcarcinomapleuralfluidhuman.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD163 antibody [BLR087G] - BSA free (ab272066)Immunohistochemical analysis of formalin-fixed, paraffin-embedded human lung carcinoma pleural fluid tissue labeling CD163 with ab265592 at 1/100 dilution. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.
This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (ab265592).
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![Anti-CD163 antibody [BLR087G] - BSA free (ab272066)](https://www.abcam.co.jp/ps/products/272/ab272066/Images/ab272066-4-benefits-of-recombinant-antibodies.png)
Anti-CD163 antibody [BLR087G] - BSA free (ab272066)
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab272066 は論文での使用が確認できていません。