Anti-CD161 抗体 [B199.2] (ab23624)
Key features and details
- Mouse monoclonal [B199.2] to CD161
- Suitable for: IHC-Fr, IP, Flow Cyt
- Reacts with: Human
- Isotype: IgG1
製品の概要
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製品名
Anti-CD161 antibody [B199.2]
CD161 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [B199.2] to CD161 -
由来種
Mouse -
特異性
ab23624 recognises CD161 cell surface antigen, expressed by almost all NK cells and with a small subset of CD3 positive T cells. -
アプリケーション
適用あり: IHC-Fr, IP, Flow Cytmore details
適用なし: WB -
種交差性
交差種: Human -
免疫原
Tissue, cells or virus corresponding to Human CD161. Activated human NK cells.
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エピトープ
Clone B199.2 recognises a similar epitope to the DX9 monoclonal antibody. -
特記事項
Myeloma cell line: P2X63.Ag8.653The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading... -
精製度
Protein G purified -
ポリ/モノ
モノクローナル -
クローン名
B199.2 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab23624の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-Fr |
Use at an assay dependent concentration.
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| IP |
Use at an assay dependent concentration.
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| Flow Cyt |
Use at an assay dependent concentration.
Use 10ul for 10^6 cells in 100ul, or 1/50 - 1/100. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
| 特記事項 |
|---|
|
IHC-Fr
Use at an assay dependent concentration. |
|
IP
Use at an assay dependent concentration. |
|
Flow Cyt
Use at an assay dependent concentration. Use 10ul for 10^6 cells in 100ul, or 1/50 - 1/100. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
ターゲット情報
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機能
Plays an inhibitory role on natural killer (NK) cells cytotoxicity. Activation results in specific acid sphingomyelinase/SMPD1 stimulation with subsequent marked elevation of intracellular ceramide. Activation also leads to AKT1/PKB and RPS6KA1/RSK1 kinases stimulation as well as markedly enhanced T-cell proliferation induced by anti-CD3. Acts as a lectin that binds to the terminal carbohydrate Gal-alpha(1,3)Gal epitope as well as to the N-acetyllactosamine epitope. Binds also to CLEC2D/LLT1 as a ligand and inhibits NK cell-mediated cytotoxicity as well as interferon-gamma secretion in target cells. -
組織特異性
Expressed in a subset of NK cells predominantly in intestinal epithelium and liver. Detected in peripheral blood T-cells and preferentially in adult T-cells with a memory antigenic phenotype. -
配列類似性
Contains 1 C-type lectin domain. -
翻訳後修飾
N-glycosylated. Contains sialic acid residues. -
細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 3820 Human
- Omim: 602890 Human
- SwissProt: Q12918 Human
- Unigene: 169824 Human
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別名
- C-type lectin domain family 5 member B antibody
- CD161 antibody
- CLEC5B antibody
see all
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (2)
ab23624 は 2 報の論文で使用されています。
- Llibre A et al. LLT1 and CD161 Expression in Human Germinal Centers Promotes B Cell Activation and CXCR4 Downregulation. J Immunol 196:2085-94 (2016). IHC . PubMed: 26829983
- Barnig C et al. Lipoxin a4 regulates natural killer cell and type 2 innate lymphoid cell activation in asthma. Sci Transl Med 5:174ra26 (2013). IHC-P ; Human . PubMed: 23447017
