Anti-CD16a 抗体 [SP189] - C-terminal (ab227665)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [SP189] to CD16a - C-terminal
- Suitable for: WB, IHC-P, Flow Cyt (Intra)
- Reacts with: Human
Related conjugates and formulations
製品の概要
-
製品名
Anti-CD16a antibody [SP189] - C-terminal
CD16a 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [SP189] to CD16a - C-terminal -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-P, Flow Cyt (Intra)more details -
種交差性
交差種: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
ポジティブ・コントロール
- IHC-P: Human lung, liver, thymus, spleen, placenta, B-cell lymphoma, cervical squamous cell carcinoma, colon carcinoma, HK lymphoma, stomach carcinoma, and liver hepatocellular carcinoma tissue; WB: K562 cell lysate; Flow Cyt (Intra): Jurkat cells.
-
特記事項
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading...
-
精製度
Protein A/G purified -
特記事項(精製)
Purified from TCS by protein A/G. -
ポリ/モノ
モノクローナル -
クローン名
SP189 -
アイソタイプ
IgG -
研究分野
関連製品
-
Alternative Versions
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab227665の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB |
1/100. Predicted molecular weight: 29 kDa.
|
|
IHC-P |
1/100.
Antigen retrieval: Boil tissue section in EDTA buffer, pH 8.0 for 10 minutes followed by cooling at |
|
Flow Cyt (Intra) |
1/100.
|
特記事項 |
---|
WB
1/100. Predicted molecular weight: 29 kDa. |
IHC-P
1/100. Antigen retrieval: Boil tissue section in EDTA buffer, pH 8.0 for 10 minutes followed by cooling at |
Flow Cyt (Intra)
1/100. |
ターゲット情報
-
機能
Receptor for the Fc region of IgG. Binds complexed or aggregated IgG and also monomeric IgG. Mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis. -
組織特異性
Expressed on natural killer cells, macrophages, subpopulation of T-cells, immature thymocytes and placental trophoblasts. -
関連疾患
Immunodeficiency 20 -
配列類似性
Contains 2 Ig-like C2-type (immunoglobulin-like) domains. -
翻訳後修飾
Glycosylated. Contains high mannose- and complex-type oligosaccharides. Glycosylation at Asn-180 is mandatory for high affinity binding to the Fc and for discrimination between fucosylated and afucosylated IgG glycoforms.
The soluble form is produced by a proteolytic cleavage. -
細胞内局在
Cell membrane. Secreted. Exists also as a soluble receptor. - Information by UniProt
-
参照データベース
- Entrez Gene: 2214 Human
- SwissProt: P08637 Human
- Unigene: 372679 Human
-
別名
- CD16a antibody
- CD16a antigen antibody
- Fc-gamma RIII antibody
see all
画像
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human liver tissue sections labeling CD16a with ab227665 at 1/100 dilution (1.47 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human spleen tissue sections labeling CD16a with ab227665 at 1/100 dilution (1.47 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
-
Flow cytometric analysis of Jurkat (human T cell leukemia cell line from peripheral blood) cell line labeling CD16a with ab227665 at 1/100 (green) compared with a rabbit isotype control (blue).
-
Anti-CD16a antibody [SP189] - C-terminal (ab227665) at 1/100 dilution + K562 (human chronic myelogenous leukemia cell line from bone marrow) cell lysate
Developed using the ECL technique.
Predicted band size: 29 kDa -
Formalin-fixed, paraffin-embedded human lung tissue stained for CD16a with ab227665 at 1/100 dilution in immunohistochemical analysis.
-
Formalin-fixed, paraffin-embedded human liver tissue stained for CD16a with ab227665 at 1/100 dilution in immunohistochemical analysis.
-
Formalin-fixed, paraffin-embedded human thymus tissue stained for CD16a with ab227665 at 1/100 dilution in immunohistochemical analysis.
-
Formalin-fixed, paraffin-embedded human liver hepatocellular carcinoma tissue stained for CD16a with ab227665 at 1/100 dilution in immunohistochemical analysis.
-
Formalin-fixed, paraffin-embedded human placenta tissue stained for CD16a with ab227665 at 1/100 dilution in immunohistochemical analysis.
-
Formalin-fixed, paraffin-embedded human B-cell lymphoma tissue stained for CD16a with ab227665 at 1/100 dilution in immunohistochemical analysis.
-
Formalin-fixed, paraffin-embedded human cervical squamous cell carcinoma tissue stained for CD16a with ab227665 at 1/100 dilution in immunohistochemical analysis.
-
Formalin-fixed, paraffin-embedded human colon adenocarcinoma tissue stained for CD16a with ab227665 at 1/100 dilution in immunohistochemical analysis.
-
Formalin-fixed, paraffin-embedded human HK lymphoma tissue stained for CD16a with ab227665 at 1/100 dilution in immunohistochemical analysis.
-
Formalin-fixed, paraffin-embedded human spleen tissue stained for CD16a with ab227665 at 1/100 dilution in immunohistochemical analysis.
-
Formalin-fixed, paraffin-embedded human stomach adenocarcinoma tissue stained for CD16a with ab227665 at 1/100 dilution in immunohistochemical analysis.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (0)
ab227665 は論文での使用が確認できていません。