Anti-CD16 + CD32 抗体 [EPR22345] (ab228971)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22345] to CD16 + CD32
- Suitable for: WB, IHC-P
- Reacts with: Mouse
Related conjugates and formulations
製品の概要
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製品名
Anti-CD16 + CD32 antibody [EPR22345] -
製品の詳細
Rabbit monoclonal [EPR22345] to CD16 + CD32 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details
適用なし: Flow Cyt,ICC/IF or IP -
種交差性
交差種: Mouse -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- WB: Mouse spleen, liver and thymus lysate. His-tagged mouse CD16 recombinant protein(aa31-215) and His-tagged mouse CD32 recombinant protein (aa30-210). J774A.1 whole cell lysate. IHC-P: Mouse liver and colon tissue.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR22345 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Immunohistochemistry reagents
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab228971の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/1000. Predicted molecular weight: 29 kDa.
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IHC-P | (1) |
1/4000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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特記事項 |
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WB
1/1000. Predicted molecular weight: 29 kDa. |
IHC-P
1/4000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ターゲット情報
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細胞内局在
CD16: Cell membrane. Secreted. Exists also as a soluble receptor. CD32: Cell membrane. -
参照データベース
- Entrez Gene: 14130 Mouse
- Entrez Gene: 14131 Mouse
- SwissProt: P08101 Mouse
- SwissProt: P08508 Mouse
画像
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All lanes : Anti-CD16 + CD32 antibody [EPR22345] (ab228971) at 1/1000 dilution
Lane 1 : Mouse spleen lysate
Lane 2 : Mouse thymus lysate
Lane 3 : Mouse liver lysate
Lane 4 : J774A.1 (mouse reticulum cell sarcoma monocyte macrophage) whole cell lysate
Lane 5 : bEnd.3 (mouse brain endothelioma) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 29 kDa
Observed band size: 30-55 kDa why is the actual band size different from the predicted?Blocking/Diluting buffer and concentration: 5% NFDM/TBST
Exposure time:
Lanes 1,2,3 and 5: 3 minutes;
Lanes 4: 15 seconds.Bands between 30-55 KD are likely to be glycosylated forms of CD16 and CD32 (PMID: 9378972, PMID: 19503602).
Negative control: bEnd.3 (PMID: 23911392)
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Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling CD16 with ab228971 at 1/4,000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic staining on Kupffer cells and sinusoidal cells of mouse liver (PMID:26512139) is observed. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). -
All lanes : Anti-CD16 + CD32 antibody [EPR22345] (ab228971) at 1/1000 dilution
Lane 1 : His-tagged mouse CD16 recombinant protein (aa31-215), 20 ng
Lane 2 : His-tagged mouse CD32 recombinant protein (aa30-210), 20 ng
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 29 kDa
Observed band size: 21 kDa why is the actual band size different from the predicted?
Exposure time: 6 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
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Immunohistochemical analysis of paraffin-embedded mouse colon tissue labeling CD16 with ab228971 at 1/4,000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic staining on stromal cells in mouse colon (PMID:26512139) is observed. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
Certificate of Compliance
参考文献 (2)
ab228971 は 2 報の論文で使用されています。
- Zhang B et al. Shionone Attenuates Sepsis-Induced Acute Kidney Injury by Regulating Macrophage Polarization via the ECM1/STAT5 Pathway. Front Med (Lausanne) 8:796743 (2021). PubMed: 35141243
- Feng YW et al. hUCMSCs Mitigate LPS-Induced Trained Immunity in Ischemic Stroke. Front Immunol 11:1746 (2020). PubMed: 33013828