Anti-CD15 抗体 [P12] (ab3358)

Submit a review Q&A (3)References (5)

Key features and details

  • Mouse monoclonal [P12] to CD15
  • Suitable for: IHC-P, ICC/IF
  • Isotype: IgM

製品の概要

  • 製品名

    Anti-CD15 antibody [P12]
    CD15 一次抗体 製品一覧

  • 製品の詳細

    Mouse monoclonal [P12] to CD15

  • 由来種

    Mouse

  • アプリケーション

    適用あり: IHC-P, ICC/IFmore details

  • 種交差性


    交差が予測される動物種: Human

  • 免疫原

    Fresh (non-cultured) placenta cell preparation (Human).

  • ポジティブ･コントロール

    • Human kidney tissue.

  • 特記事項


    Blood group related antigens represent a group of carbohydrate determinants carried on both glycolipids and glycoproteins. They are usually mucin-type, and are detected on erythrocytes, certain epithelial cells, and in secretions of certain individuals. Sixteen genetically and biosynthetically distinct but inter-related specificities belong to this group of antigens, including A, B, H, Lewis a, Lewis b, Lewis x, Lewis y, and precursor type 1 chain antigens. This antibody belongs to the BG-7 group and detects the Lewis x (type 2 chain) blood-group antigen, which is expressed in gastrointestinal tumors, and lung cancers. It is found in crypt cells, which represent the proliferative compartment of the colonic mucosa, and strong homogeneous immunostaining is observed in most metastases and invasive cancer cells.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab3358の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IHC-P
1/40.
ICC/IF
1/200 - 1/400.
特記事項
IHC-P
1/40.
ICC/IF
1/200 - 1/400.

ターゲット情報

  • 関連性

    CD15 is a carbohydrate adhesion molecule (and not a protein) that mediates phagocytosis and chemotaxis. Synthesis is directed by FUT4 in lymphoid cells and mature granulocytes, and by FUT9 in promyelocytes and monocytes.

  • 細胞内局在

    Golgi Apparatus; Membrane-bound form in trans cisternae of Golgi.

  • 別名

    • 3 fucosyl N acetyl lactosamine antibody
    • 3-FAL antibody
    • 3-fucosyl-N-acetyl-lactosamine antibody
    • Leu M1 antibody
    • Lewis X antibody
    • LeX antibody
    • SSEA-1 antibody
    • stage-specific embryonic antigen 1 antibody
    see all

参考文献 (5)

ab3358 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab3358 は 5 報の論文で使用されています。

  • Lu HH  et al. Fucosyltransferase 4 shapes oncogenic glycoproteome to drive metastasis of lung adenocarcinoma. EBioMedicine 57:102846 (2020). PubMed: 32629386
  • Purohit S  et al. Multiplex glycan bead array for high throughput and high content analyses of glycan binding proteins. Nat Commun 9:258 (2018). PubMed: 29343722
  • Morelle W  et al. Glycosylation pattern of brush border-associated glycoproteins in enterocyte-like cells: involvement of complex-type N-glycans in apical trafficking. Biol Chem 390:529-44 (2009). PubMed: 19426135
  • Shi CS  et al. Lectin-like domain of thrombomodulin binds to its specific ligand Lewis Y antigen and neutralizes lipopolysaccharide-induced inflammatory response. Blood 112:3661-70 (2008). PubMed: 18711002
  • Nyström K  et al. Virus-induced transcriptional activation of host FUT genes associated with neo-expression of Ley in cytomegalovirus-infected and sialyl-Lex in varicella-zoster virus-infected diploid human cells. Glycobiology 17:355-66 (2007). PubMed: 17202152

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1-3 of 3 Abreviews or Q&A

Question

Would you kindly indicate the long term storage of the following items:

ab9100

ab3358

ab3359

ab30667

ab86128



We noticed that for example #ab9100 is shipped by 4°C but has to be stored at -20°C !!!

From which day the long term storage applays?



Further more, would you please arrange that this information will be printed on the vials ???!!!

Thank you -

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Answer

Thank you for your inquiry.

I can confirm that the storage temperature of our products can be found on the individual datasheets.

Short term storage is usually two weeks. If the products is not used within two weeks of purchase, we in general recommend to aliquot it and to store as stated for the long term storage. As a general rule, freeze/thaw cycle are to be avoided.

Please find below the long term storage conditions for the products you are interested in:

ab9100 : Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

ab3358 : Store at +4°C.

ab3359 : Store at +4°C.

ab30667 : Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

ab86128 : Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Since the vials are small and we want the text to be readable, we strongly suggest to check the datasheets for the storage information. This will have the positive side effect that also all the other valuable information on the datasheet will be seen.

I hope this information is helpful and wish you a sunny day.

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Question

Product code: 3358
Lot number: GR31367-2

Inquiry: Dear Abcam, I recently received an antibody against LeX from Abcam. Could you please tell me what the concentration of the antibody is? Reference number: ab3358 Lot number: GR31367-2 Kind regards,

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Answer

Thank you for your enquiry.

I can confirm that ab3358Blood Group Lewis x antibody [P12] is sold as tissue culture supernatant. I have requested that the datasheetisupdated so thisinformation is clearer. Unpurified antibodies, such as those sold as whole antiserum, ascites or tissue culture supernatant will not have a concentration stated on the datasheet. Antibody concentration is usually determined by protein assay,and tissue culture supernatant will contain a lot of other proteins, which means the antibody quantification would not be accurate.

I can confirm that for this particular product, theconcentration is estimated at 10-50ug/ml.

I am sorry we are not able to provide an exact concentration on this occasion, but hope this information will be helpful to you. If you have any further questions, please do not hesitate to contact us.

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Question

Was this antibody purified from ascites or tissue culture supernatant? What is the concentration?

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Answer

Thank you for your enquiry. This antibody is tissue culture supernatant concentrated by ammonium sulfate precipitation. The concentration of our last lot was 180 ug/ml. We don't currently have any stock of this antibody, so the concentration may change with new stock. I hope this information helps. Please do not hesitate to contact us if you need anything further.

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