Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Cord blood)
Specification
Cord blood
Fixative
Methanol
Blocking step
Serum as blocking agent for 15 minute(s) · Concentration: 10%
Other product details
Dilution
1/1000
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 24°C
Secondary antibody
Name
Non-Abcam antibody was used: Alexa Fluro 568
Host species: Donkey
Clonality: Monoclonal
Conjugation: Alexa Fluor® 568
Host species: Donkey
Clonality: Monoclonal
Conjugation: Alexa Fluor® 568
Dilution
1/1000
Additional data
Additional Notes
CD15 expressed in stem cells of myeloid lineage in bone marrow and cord blood. Here we identified CD15 positive human cord blood cells using the ab665 primary antibody and Alexa Flour 568 secondary antibody.
Hoechst-blue was also applied to identify nucleated cells. CD15 expressed in neutrophils and in stem cells.
Hoechst-blue was also applied to identify nucleated cells. CD15 expressed in neutrophils and in stem cells.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Ruma Raha-Chowdhury
Verified customer
投稿 May 25 2011