Anti-CD15 抗体 [BRA-4F1] - BSA and Azide free (ab269779)
Key features and details
- Mouse monoclonal [BRA-4F1] to CD15 - BSA and Azide free
- Suitable for: IHC-P
- Reacts with: Species independent
- Isotype: IgM
製品の概要
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製品名
Anti-CD15 antibody [BRA-4F1] - BSA and Azide free
CD15 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [BRA-4F1] to CD15 - BSA and Azide free -
由来種
Mouse -
アプリケーション
適用あり: IHC-Pmore details -
種交差性
交差種: Species independent -
免疫原
Tissue, cells or virus corresponding to Human CD15. (Myelomonocytic leukemia cells).
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ポジティブ･コントロール
- IHC-P: Human Hodgkin's lymphoma tissue.
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特記事項
ab269779 is a carrier free version of ab233868. This format is designed for use in antibody labeling, including fluorochromes, metal isotypes, oligonucleotides, enzymes.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Constituent: PBS -
キャリア・フリー
はい -
Concentration information loading... -
特記事項（精製）
Purified from Bioreactor Concentrate. -
ポリ/モノ
モノクローナル -
クローン名
BRA-4F1 -
アイソタイプ
IgM -
軽鎖の種類
kappa -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab269779 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P |
Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Primary incubation for 30 minutes at RT.
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| 特記事項 |
|---|
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IHC-P
Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. Primary incubation for 30 minutes at RT.
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ターゲット情報
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関連性
CD15 is a carbohydrate adhesion molecule (and not a protein) that mediates phagocytosis and chemotaxis. Synthesis is directed by FUT4 in lymphoid cells and mature granulocytes, and by FUT9 in promyelocytes and monocytes. -
細胞内局在
Golgi Apparatus; Membrane-bound form in trans cisternae of Golgi. -
別名
- 3 fucosyl N acetyl lactosamine antibody
- 3-FAL antibody
- 3-fucosyl-N-acetyl-lactosamine antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD15 antibody (ab269779)
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab269779 は論文での使用が確認できていません。