Anti-CD147 抗体 [MEM-M6/1] (ab666)
製品の概要
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製品名Anti-CD147 antibody [MEM-M6/1]
CD147 一次抗体 製品一覧 -
製品の詳細Mouse monoclonal [MEM-M6/1] to CD147
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由来種Mouse
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特異性Human CD147 antigen. This antibody recognizes an epitope in the N-terminal Ig domain (D1). This high-affinity antibody is capable of binding to unstimulated peripheral blood T cells.
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アプリケーション適用あり: IHC-P, Flow Cyt, IP, WBmore details
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種交差性交差種: Human
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免疫原
Recombinant full length protein corresponding to Human CD147. Purified soluble recombinant form of CD147, CD147Rg, which consists of the cDNA coding for the hinge region, CH2-and CH3 domain of human IgG1 (CD147Rg is secreted by transfectants as a dimer).
Database link: P35613 -
ポジティブ・コントロール
- This antibody gave a positive result in IHC in the following FFPE tissue: Human normal heart muscle. Flow Cytometry: Peripheral blood lymphocytes.
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特記事項
This product was changed from ascites to tissue culture supernatant on 24th January 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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バッファーpH: 7.40
Preservative: 0.097% Sodium azide
Constituent: PBS -
Concentration information loading...
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精製度>95% by SDS-PAGE
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特記事項(精製)Purified from TCS
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ポリ/モノモノクローナル
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クローン名MEM-M6/1
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アイソタイプIgG1
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研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
アプリケーション
Our Abpromise guarantee covers the use of ab666 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
アプリケーション | Abreviews | 特記事項 |
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IHC-P | Use a concentration of 10 µg/ml. | |
Flow Cyt | Use 1µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
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IP | Use at an assay dependent concentration. | |
WB | Use a concentration of 1 µg/ml. Use under non reducing condition. |
ターゲット情報
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機能Plays pivotal roles in spermatogenesis, embryo implantation, neural network formation and tumor progression. Stimulates adjacent fibroblasts to produce matrix metalloproteinases (MMPS). May target monocarboxylate transporters SLC16A1, SLC16A3 and SLC16A8 to plasma membranes of retinal pigment epithelium and neural retina. Seems to be a receptor for oligomannosidic glycans. In vitro, promotes outgrowth of astrocytic processes.
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組織特異性Present only in vascular endothelium in non-neoplastic regions of the brain, whereas it is present in tumor cells but not in proliferating blood vessels in malignant gliomas.
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配列類似性Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
翻訳後修飾N-glycosylated.
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細胞内局在Cell membrane. Melanosome. Colocalizes with SLC16A1 and SLC16A8 (By similarity). Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
- Information by UniProt
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参照データベース
- Entrez Gene: 682 Human
- Omim: 109480 Human
- SwissProt: P35613 Human
- Unigene: 501293 Human
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別名
- 5A11 antigen antibody
- 5F7 antibody
- BASI_HUMAN antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD147 antibody [MEM-M6/1] (ab666)
IHC image of CD147 staining in human normal heart muscle formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6, epitope retrieval solution 1) for 20 minutes. The section was then incubated with ab666, 5 µg/ml, for 15 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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Flow cytometry of human peripheral blood cells with ab666 at 1 µg/ml
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Overlay histogram showing peripheral blood lymphocytes stained with ab666 (red line). The cells were incubated with the antibody (ab666, 1 µg/1x106 cells) for 30 minutes at 4ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 minutes at 4ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2 µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed gating on peripheral blood lymphocytes.
プロトコール
参考文献
This product has been referenced in:
- Badillo-Soto MA et al. Potential protein targets of the peptidylarginine deiminase 2 and peptidylarginine deiminase 4 enzymes in rheumatoid synovial tissue and its possible meaning. Eur J Rheumatol 3:44-49 (2016). Read more (PubMed: 27708970) »
- Huang XQ et al. Co-expression of CD147 and GLUT-1 indicates radiation resistance and poor prognosis in cervical squamous cell carcinoma. Int J Clin Exp Pathol 7:1651-66 (2014). Read more (PubMed: 24817962) »