Anti-CD14 抗体 [UCHM1] (ab6623)

Submit a review Q&A (1)
Flow Cytometry - Anti-CD14 antibody [UCHM1] (ab6623)

    Key features and details

    • Mouse monoclonal [UCHM1] to CD14
    • Suitable for: Flow Cyt
    • Reacts with: Human, Cynomolgus monkey, Rhesus monkey
    • Isotype: IgG2a

    製品の概要

    • 製品名

      Anti-CD14 antibody [UCHM1]
      CD14 一次抗体 製品一覧

    • 製品の詳細

      Mouse monoclonal [UCHM1] to CD14

    • 由来種

      Mouse

    • 特異性

      ab6623 recognises a monocyte surface antigen of 55 kDa found chiefly on monocytes. UCHM1 was clustered at the Third International Workshop and Conference on Human Leucocyte Differentiation Antigens. Oxford 1986, as CDw14, group A. In tonsil tissue sections UCHM1 gives positive staining reactions with monocytic cells, the interfollicular tissue macrophages seen under the capsule, and dendritic reticulum cells. Skin Langerhans cells are always negative. UCHM1 also reacts with Kupffer cells and sinus lining cells on the liver.

    • アプリケーション

      適用あり: Flow Cytmore details
      適用なし: IHC-P

    • 種交差性

      交差種: Human, Cynomolgus monkey, Rhesus monkey

    • 免疫原

      Human Thymocytes followed by peripheral blood mononuclear cells

    • ポジティブ･コントロール

      • Tonsil.

    • 特記事項

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    製品の特性

    アプリケーション

    Our Abpromise guarantee covers the use of ab6623 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    アプリケーション Abreviews 特記事項
    Flow Cyt Use at an assay dependent concentration.

    ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

     
  • 追加情報
    Is unsuitable for IHC-P.

    • ターゲット情報

    • 機能

      Cooperates with MD-2 and TLR4 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Up-regulates cell surface molecules, including adhesion molecules.

    • 組織特異性

      Expressed strongly on the surface of monocytes and weakly on the surface of granulocytes; also expressed by most tissue macrophages.

    • 配列類似性

      Contains 11 LRR (leucine-rich) repeats.

    • 翻訳後修飾

      N- and O- glycosylated. O-glycosylated with a core 1 or possibly core 8 glycan.

    • 細胞内局在

      Cell membrane.
    • Information by UniProt

    • 参照データベース

      • 別名

        • CD 14 antibody
        • CD_antigen=CD14 antibody
        • CD14 antibody
        • CD14 antigen antibody
        • CD14 molecule antibody
        • CD14_HUMAN antibody
        • LPS-R antibody
        • Mo2 antibody
        • Monocyte differentiation antigen CD14 antibody
        • Monocyte differentiation antigen CD14 urinary form antibody
        • Monocyte differentiation antigen CD14, membrane-bound form antibody
        • Myeloid cell specific leucine rich glycoprotein antibody
        • Myeloid cell-specific leucine-rich glycoprotein antibody
        see all

      画像

      • Flow Cytometry - Anti-CD14 antibody [UCHM1] (ab6623)
        Flow Cytometry - Anti-CD14 antibody [UCHM1] (ab6623)
        ab6623 staining of human peripheral blood monocytes, visualised with rabbit anti-mouse IgG: FITC.

      参考文献 (0)

      ab6623 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

      ab6623 は論文での使用が確認できていません。

      レビューと Q&A

      Show All レビュー Q&A
      レビューを送る 質問を送る

      Question

      Can this antibody be used with paraffin sections or only frozen as stated on the datasheet?

      Read More
      Answer

      This antibody has been tested on paraffin sections and does not work. In IHC, it is known to work only with frozen sections.

      Read More

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com