Anti-CD14 抗体 [MEM-18] - Low endotoxin, Azide free (ab6083)
![Flow Cytometry - Anti-CD14 antibody [MEM-18] - Low endotoxin, Azide free (ab6083)](https://www.abcam.co.jp/ps/products/6/ab6083/Images/ab6083-199461-anti-cd14-antibody-mem-18-flow-cytometry.jpg "Flow Cytometry - Anti-CD14 antibody [MEM-18] - Low endotoxin, Azide free (ab6083)")
Key features and details
- Mouse monoclonal [MEM-18] to CD14 - Low endotoxin, Azide free
- Suitable for: Flow Cyt
- Reacts with: Human
- Isotype: IgG1
Related conjugates and formulations
製品の概要
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製品名
Anti-CD14 antibody [MEM-18] - Low endotoxin, Azide free
CD14 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [MEM-18] to CD14 - Low endotoxin, Azide free -
由来種
Mouse -
アプリケーション
適用あり: Flow Cytmore details -
種交差性
交差種: Human
交差が予測される動物種: Monkey, Non human primates
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免疫原
Full length protein corresponding to Human CD14. A crude mixture of human urinary proteins precipitated by ammonium sulphate from the urine of a patient suffering from proteinuria.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.40
Constituent: PBS -
キャリア・フリー
はい -
Concentration information loading... -
精製度
Tissue culture supernatant -
ポリ/モノ
モノクローナル -
クローン名
MEM-18 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab6083の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Flow Cyt |
Use 0.01µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
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| 特記事項 |
|---|
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Flow Cyt
Use 0.01µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
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ターゲット情報
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機能
Cooperates with MD-2 and TLR4 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Up-regulates cell surface molecules, including adhesion molecules. -
組織特異性
Expressed strongly on the surface of monocytes and weakly on the surface of granulocytes; also expressed by most tissue macrophages. -
配列類似性
Contains 11 LRR (leucine-rich) repeats. -
翻訳後修飾
N- and O- glycosylated. O-glycosylated with a core 1 or possibly core 8 glycan. -
細胞内局在
Cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 929 Human
- Omim: 158120 Human
- SwissProt: P08571 Human
- Unigene: 163867 Human
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別名
- CD 14 antibody
- CD_antigen=CD14 antibody
- CD14 antibody
see all
画像
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Flow Cytometry - Anti-CD14 antibody [MEM-18] - Low endotoxin, Azide free (ab6083)
Human peripheral blood cells stained with ab6083 (red line). Human whole blood was processed using a modified protocol based on Chow et al, 2005 (PMID: 16080188). In brief, human whole blood was fixed in 4% formaldehyde (methanol-free) for 10 min at 22°C. Red blood cells were then lyzed by the addition of Triton X-100 (final concentration - 0.1%) for 15 min at 37°C. For experimentation, cells were treated with 50% methanol (-20°C) for 15 min at 4°C. Cells were then incubated with the antibody (ab6083, 0.01μg/1x106 cells) for 30 min at 4°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 4°C. Acquisition of >30,000 total events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
データシートおよび資料
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Datasheet download
参考文献 (8)
ab6083 は 8 報の論文で使用されています。
- van Montfoort N et al. Hepatitis B Virus Surface Antigen Activates Myeloid Dendritic Cells via a Soluble CD14-Dependent Mechanism. J Virol 90:6187-99 (2016). PubMed: 27099316
- Landa-Solís C et al. Cryopreserved CD90+ cells obtained from mobilized peripheral blood in sheep: a new source of mesenchymal stem cells for preclinical applications. Cell Tissue Bank : (2015). IF . PubMed: 26220398
- Suzuki K et al. Human anti-microbial cathelicidin peptide LL-37 suppresses the LPS-induced apoptosis of endothelial cells. Int Immunol 23:185-93 (2011). PubMed: 21393634
- He J et al. Mycoplasma genitalium-derived lipid-associated membrane proteins activate NF-kappaB through toll-like receptors 1, 2, and 6 and CD14 in a MyD88-dependent pathway. Clin Vaccine Immunol 16:1750-7 (2009). PubMed: 19793902
- Maurice S et al. Isolation of progenitor cells from cord blood using adhesion matrices. Cytotechnology 54:121-33 (2007). PubMed: 19003027
- Sundan A et al. Soluble CD14 from urine copurifies with a potent inducer of cytokines. Eur J Immunol 24:1779-84 (1994). PubMed: 7519994
- Bazil V et al. Structural relationship between the soluble and membrane-bound forms of human monocyte surface glycoprotein CD14. Mol Immunol 26:657-62 (1989). PubMed: 2779588
- Bazil V et al. Biochemical characterization of a soluble form of the 53-kDa monocyte surface antigen. Eur J Immunol 16:1583-9 (1986). PubMed: 3493149
