Anti-CD14 抗体 [MEM-18] - Low endotoxin, Azide free (ab6083)

Submit a review Q&A (2)References (8)
Flow Cytometry - Anti-CD14 antibody [MEM-18] - Low endotoxin, Azide free (ab6083)

    Key features and details

    • Mouse monoclonal [MEM-18] to CD14 - Low endotoxin, Azide free
    • Suitable for: Flow Cyt
    • Reacts with: Human
    • Isotype: IgG1

    Conjugates logo Related conjugates and formulations

    Alexa Fluor® 700 APC Biotin FITC

    製品の概要

    • 製品名

      Anti-CD14 antibody [MEM-18] - Low endotoxin, Azide free
      CD14 一次抗体 製品一覧

    • 製品の詳細

      Mouse monoclonal [MEM-18] to CD14 - Low endotoxin, Azide free

    • 由来種

      Mouse

    • アプリケーション

      適用あり: Flow Cytmore details

    • 種交差性

      交差種: Human
      交差が予測される動物種: Monkey, Non human primates

    • 免疫原

      Full length protein corresponding to Human CD14. A crude mixture of human urinary proteins precipitated by ammonium sulphate from the urine of a patient suffering from proteinuria.

    • 特記事項

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    製品の特性

    アプリケーション

    The Abpromise guarantee

    Abpromise保証は、 次のテスト済みアプリケーションにおけるab6083の使用に適用されます

    アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

    アプリケーション Abreviews 特記事項
    Flow Cyt
    Use 0.01µg for 106 cells.

    ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

     
    特記事項
    Flow Cyt
    Use 0.01µg for 106 cells.

    ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

     

    ターゲット情報

    • 機能

      Cooperates with MD-2 and TLR4 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Up-regulates cell surface molecules, including adhesion molecules.

    • 組織特異性

      Expressed strongly on the surface of monocytes and weakly on the surface of granulocytes; also expressed by most tissue macrophages.

    • 配列類似性

      Contains 11 LRR (leucine-rich) repeats.

    • 翻訳後修飾

      N- and O- glycosylated. O-glycosylated with a core 1 or possibly core 8 glycan.

    • 細胞内局在

      Cell membrane.
    • Information by UniProt

    • 参照データベース

      • 別名

        • CD 14 antibody
        • CD_antigen=CD14 antibody
        • CD14 antibody
        • CD14 antigen antibody
        • CD14 molecule antibody
        • CD14_HUMAN antibody
        • LPS-R antibody
        • Mo2 antibody
        • Monocyte differentiation antigen CD14 antibody
        • Monocyte differentiation antigen CD14 urinary form antibody
        • Monocyte differentiation antigen CD14, membrane-bound form antibody
        • Myeloid cell specific leucine rich glycoprotein antibody
        • Myeloid cell-specific leucine-rich glycoprotein antibody
        see all

      画像

      • Flow Cytometry - Anti-CD14 antibody [MEM-18] - Low endotoxin, Azide free (ab6083)
        Flow Cytometry - Anti-CD14 antibody [MEM-18] - Low endotoxin, Azide free (ab6083)

        Human peripheral blood cells stained with ab6083 (red line). Human whole blood was processed using a modified protocol based on Chow et al, 2005 (PMID: 16080188). In brief, human whole blood was fixed in 4% formaldehyde (methanol-free) for 10 min at 22°C. Red blood cells were then lyzed by the addition of Triton X-100 (final concentration - 0.1%) for 15 min at 37°C. For experimentation, cells were treated with 50% methanol (-20°C) for 15 min at 4°C. Cells were then incubated with the antibody (ab6083, 0.01μg/1x106 cells) for 30 min at 4°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 4°C. Acquisition of >30,000 total events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

      参考文献 (8)

      ab6083 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

      ab6083 は 8 報の論文で使用されています。

      • van Montfoort N  et al. Hepatitis B Virus Surface Antigen Activates Myeloid Dendritic Cells via a Soluble CD14-Dependent Mechanism. J Virol 90:6187-99 (2016). PubMed: 27099316
      • Landa-Solís C  et al. Cryopreserved CD90+ cells obtained from mobilized peripheral blood in sheep: a new source of mesenchymal stem cells for preclinical applications. Cell Tissue Bank : (2015). IF . PubMed: 26220398
      • Suzuki K  et al. Human anti-microbial cathelicidin peptide LL-37 suppresses the LPS-induced apoptosis of endothelial cells. Int Immunol 23:185-93 (2011). PubMed: 21393634
      • He J  et al. Mycoplasma genitalium-derived lipid-associated membrane proteins activate NF-kappaB through toll-like receptors 1, 2, and 6 and CD14 in a MyD88-dependent pathway. Clin Vaccine Immunol 16:1750-7 (2009). PubMed: 19793902
      • Maurice S  et al. Isolation of progenitor cells from cord blood using adhesion matrices. Cytotechnology 54:121-33 (2007). PubMed: 19003027
      • Sundan A  et al. Soluble CD14 from urine copurifies with a potent inducer of cytokines. Eur J Immunol 24:1779-84 (1994). PubMed: 7519994
      • Bazil V  et al. Structural relationship between the soluble and membrane-bound forms of human monocyte surface glycoprotein CD14. Mol Immunol 26:657-62 (1989). PubMed: 2779588
      • Bazil V  et al. Biochemical characterization of a soluble form of the 53-kDa monocyte surface antigen. Eur J Immunol 16:1583-9 (1986). PubMed: 3493149

      レビューと Q&A

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      Question

      will this detect membrane AND soluble CD14?

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      Answer

      The Anti-CD14 antibody [MEM-18] - Azide free (ab6083) will react with both membrane and soluble CD14.



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      Question

      Product code: 6083
      Lot number: GR109154-1
      Inquiry: Hello, today I received my mouse anti human CD14 that I intend to use for western blotting. I wish to aliquot and store the antibodies for future use. I am quite new to western blotting so I wanted to know at what concentration I should store them at and what concentration I should use them at. Cheers,

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      Answer

      Please make 10 aliquots 10ul each. Do not dilute the antibody.

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