Anti-CD14 抗体 [MEM-18] - Low endotoxin, Azide free (ab6083)

Submit a review Q&A (2)References (8)

Key features and details

  • Mouse monoclonal [MEM-18] to CD14 - Low endotoxin, Azide free
  • Suitable for: Flow Cyt, IP, WB, ELISA, Functional Studies
  • Reacts with: Human
  • Isotype: IgG1

製品の概要

  • 製品名

    Anti-CD14 antibody [MEM-18] - Low endotoxin, Azide free
    CD14 一次抗体 製品一覧

  • 製品の詳細

    Mouse monoclonal [MEM-18] to CD14 - Low endotoxin, Azide free

  • 由来種

    Mouse

  • アプリケーション

    適用あり: Flow Cyt, IP, WB, ELISA, Functional Studiesmore details

  • 種交差性

    交差種: Human
    交差が予測される動物種: Monkey, Non human primates

  • 免疫原

    A crude mixture of human urinary proteins precipitated by ammonium sulphate from the urine of a patient suffering from proteinuria.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab6083 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt Use 0.01µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 
IP Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Use under non reducing condition. Predicted molecular weight: 45 kDa.
ELISA Use at an assay dependent concentration.
Functional Studies Use at an assay dependent concentration. Blocks binding of bacterial LPS to the monocyte surface.

ターゲット情報

  • 機能

    Cooperates with MD-2 and TLR4 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Up-regulates cell surface molecules, including adhesion molecules.

  • 組織特異性

    Expressed strongly on the surface of monocytes and weakly on the surface of granulocytes; also expressed by most tissue macrophages.

  • 配列類似性

    Contains 11 LRR (leucine-rich) repeats.

  • 翻訳後修飾

    N- and O- glycosylated. O-glycosylated with a core 1 or possibly core 8 glycan.

  • 細胞内局在

    Cell membrane.
  • Information by UniProt

  • 参照データベース

    • 別名

      • CD 14 antibody
      • CD_antigen=CD14 antibody
      • CD14 antibody
      • CD14 antigen antibody
      • CD14 molecule antibody
      • CD14_HUMAN antibody
      • LPS-R antibody
      • Mo2 antibody
      • Monocyte differentiation antigen CD14 antibody
      • Monocyte differentiation antigen CD14 urinary form antibody
      • Monocyte differentiation antigen CD14, membrane-bound form antibody
      • Myeloid cell specific leucine rich glycoprotein antibody
      • Myeloid cell-specific leucine-rich glycoprotein antibody
      see all

    画像

    • Flow Cytometry - Anti-CD14 antibody [MEM-18] - Low endotoxin, Azide free (ab6083)
      Flow Cytometry - Anti-CD14 antibody [MEM-18] - Low endotoxin, Azide free (ab6083)

      Human peripheral blood cells stained with ab6083 (red line). Human whole blood was processed using a modified protocol based on Chow et al, 2005 (PMID: 16080188). In brief, human whole blood was fixed in 4% formaldehyde (methanol-free) for 10 min at 22°C. Red blood cells were then lyzed by the addition of Triton X-100 (final concentration - 0.1%) for 15 min at 37°C. For experimentation, cells were treated with 50% methanol (-20°C) for 15 min at 4°C. Cells were then incubated with the antibody (ab6083, 0.01μg/1x106 cells) for 30 min at 4°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 4°C. Acquisition of >30,000 total events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

    参考文献 (8)

    ab6083 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab6083 は 8 報の論文で使用されています。

    • van Montfoort N  et al. Hepatitis B Virus Surface Antigen Activates Myeloid Dendritic Cells via a Soluble CD14-Dependent Mechanism. J Virol 90:6187-99 (2016). PubMed: 27099316
    • Landa-Solís C  et al. Cryopreserved CD90+ cells obtained from mobilized peripheral blood in sheep: a new source of mesenchymal stem cells for preclinical applications. Cell Tissue Bank : (2015). IF . PubMed: 26220398
    • Suzuki K  et al. Human anti-microbial cathelicidin peptide LL-37 suppresses the LPS-induced apoptosis of endothelial cells. Int Immunol 23:185-93 (2011). PubMed: 21393634
    • He J  et al. Mycoplasma genitalium-derived lipid-associated membrane proteins activate NF-kappaB through toll-like receptors 1, 2, and 6 and CD14 in a MyD88-dependent pathway. Clin Vaccine Immunol 16:1750-7 (2009). PubMed: 19793902
    • Maurice S  et al. Isolation of progenitor cells from cord blood using adhesion matrices. Cytotechnology 54:121-33 (2007). PubMed: 19003027
    • Sundan A  et al. Soluble CD14 from urine copurifies with a potent inducer of cytokines. Eur J Immunol 24:1779-84 (1994). PubMed: 7519994
    • Bazil V  et al. Structural relationship between the soluble and membrane-bound forms of human monocyte surface glycoprotein CD14. Mol Immunol 26:657-62 (1989). PubMed: 2779588
    • Bazil V  et al. Biochemical characterization of a soluble form of the 53-kDa monocyte surface antigen. Eur J Immunol 16:1583-9 (1986). PubMed: 3493149

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    Question

    will this detect membrane AND soluble CD14?

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    Answer

    The Anti-CD14 antibody [MEM-18] - Azide free (ab6083) will react with both membrane and soluble CD14.



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    Question

    Product code: 6083
    Lot number: GR109154-1
    Inquiry: Hello, today I received my mouse anti human CD14 that I intend to use for western blotting. I wish to aliquot and store the antibodies for future use. I am quite new to western blotting so I wanted to know at what concentration I should store them at and what concentration I should use them at. Cheers,

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    Answer

    Please make 10 aliquots 10ul each. Do not dilute the antibody.

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