Anti-CD14 抗体 (ab45870)
Key features and details
- Goat polyclonal to CD14
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-CD14 antibody
CD14 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to CD14 -
由来種
Goat -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide within Human CD14 aa 50-150 (internal sequence). The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
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ポジティブ・コントロール
- WB: Human lymph node lysate and human tonsil lysate.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituent: 0.5% BSA -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab45870の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | (1) |
Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 40 kDa).
A 1 hour primary incubation is recommended for this product. The observed molecular weight corresponds to earlier findings in literature with different antibodies (Funda et al, Infect Immun. 2001 Jun;69(6):3772-81; PMID: 11349042). |
特記事項 |
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WB
Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 40 kDa). A 1 hour primary incubation is recommended for this product. The observed molecular weight corresponds to earlier findings in literature with different antibodies (Funda et al, Infect Immun. 2001 Jun;69(6):3772-81; PMID: 11349042). |
ターゲット情報
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機能
Cooperates with MD-2 and TLR4 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Up-regulates cell surface molecules, including adhesion molecules. -
組織特異性
Expressed strongly on the surface of monocytes and weakly on the surface of granulocytes; also expressed by most tissue macrophages. -
配列類似性
Contains 11 LRR (leucine-rich) repeats. -
翻訳後修飾
N- and O- glycosylated. O-glycosylated with a core 1 or possibly core 8 glycan. -
細胞内局在
Cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 929 Human
- Omim: 158120 Human
- SwissProt: P08571 Human
- Unigene: 163867 Human
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別名
- CD 14 antibody
- CD_antigen=CD14 antibody
- CD14 antibody
see all
画像
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All lanes : Anti-CD14 antibody (ab45870) at 2 µg/ml
Lane 1 : Human lymph node lysate
Lane 2 : Human lymph node lysate with Immunizing peptide
Lane 3 : Human tonsil lysate
Lane 4 : Human tonsil lysate with Immunizing peptide
Lysates/proteins at 35 µg per lane.
Predicted band size: 40 kDa
Additional bands at: 55 kDa (possible glycosylated form)Primary incubation 1 hour at room temperature. Detected by chemiluminescence. RIPA buffer used.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (12)
ab45870 は 12 報の論文で使用されています。
- Tanoue S et al. Role of apoptosis inhibitor of macrophages in patients with IgG4‑related disease/autoimmune pancreatitis and the clinical characteristics associated with this condition. Biomed Rep 17:82 (2022). PubMed: 36158321
- Liu M et al. Myeloid-derived suppressor cells in gastroenteropancreatic neuroendocrine neoplasms. Endocrine 71:242-252 (2021). PubMed: 33010002
- Aguirre LA et al. Tumor stem cells fuse with monocytes to form highly invasive tumor-hybrid cells. Oncoimmunology 9:1773204 (2020). PubMed: 32923132
- Hu R et al. Construction of a sensitive pyrogen-testing cell model by site-specific knock-in of multiple genes. Biotechnol Bioeng 116:2652-2661 (2019). PubMed: 31180145
- Han Q et al. Application of a TLR overexpression cell model in pyrogen detection. Biotechnol Bioeng 116:1269-1279 (2019). PubMed: 30684361