Anti-CD14 抗体 [4B4F12] (ab182032)
Key features and details
- Mouse monoclonal [4B4F12] to CD14
- Suitable for: WB, IHC-P, Flow Cyt
- Reacts with: Mouse, Human
- Isotype: IgG1
製品の概要
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製品名
Anti-CD14 antibody [4B4F12]
CD14 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [4B4F12] to CD14 -
由来種
Mouse -
アプリケーション
適用あり: WB, IHC-P, Flow Cytmore details -
種交差性
交差種: Mouse, Human -
免疫原
Recombinant fragment corresponding to Human CD14 aa 1-250. Expressed in E. coli.
Database link: P08571 -
ポジティブ・コントロール
- Human CD14 recombinant protein (amino acids 20-214), lysate from HEK293 cells transfected with CD14 (amino acids 20-214)-hIgGFc, HepG2, A549, HL60, mouse RAW264.7, Hela, HEK293 and mouse NIH 3T3 cell lysates. Jurkat cells. Human stomach cancer tissue.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.05% Sodium azide
Constituent: 99% PBS -
Concentration information loading...
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精製度
Protein G purified -
特記事項(精製)
Purified from tissue culture supernatant. -
ポリ/モノ
モノクローナル -
クローン名
4B4F12 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab182032の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/500 - 1/2000. Predicted molecular weight: 40 kDa.
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IHC-P | (1) |
1/200 - 1/1000.
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Flow Cyt |
1/200 - 1/400.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
特記事項 |
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WB
1/500 - 1/2000. Predicted molecular weight: 40 kDa. |
IHC-P
1/200 - 1/1000. |
Flow Cyt
1/200 - 1/400. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
ターゲット情報
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機能
Cooperates with MD-2 and TLR4 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Up-regulates cell surface molecules, including adhesion molecules. -
組織特異性
Expressed strongly on the surface of monocytes and weakly on the surface of granulocytes; also expressed by most tissue macrophages. -
配列類似性
Contains 11 LRR (leucine-rich) repeats. -
翻訳後修飾
N- and O- glycosylated. O-glycosylated with a core 1 or possibly core 8 glycan. -
細胞内局在
Cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 929 Human
- Entrez Gene: 12475 Mouse
- Omim: 158120 Human
- SwissProt: P08571 Human
- SwissProt: P10810 Mouse
- Unigene: 163867 Human
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別名
- CD 14 antibody
- CD_antigen=CD14 antibody
- CD14 antibody
see all
画像
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Flow cytometric analysis of Jurkat cells labeling CD14 using ab182032 at 1/200 (green) and a negative control (red).
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Immunohistochemical analysis of paraffin-embedded, DAB-stained Human stomach cancer tissues labeling CD14 using ab182032 at 1/200.
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Anti-CD14 antibody [4B4F12] (ab182032) at 1/500 dilution + Human CD14 recombinant protein (amino acids 20-214)
Predicted band size: 40 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse liver tissue section labeling CD14 with ab182032. The tissue was fixed with formaldehyde, heat mediated antigen retrieval was performed followed by blocking with 5% serum for 12 hours at 4°C. The tissue was incubated with undiluted Anti-CD14 antibody [4B4F12] (ab182032) for 12 hours at 4°C.
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All lanes : Anti-CD14 antibody [4B4F12] (ab182032) at 1/500 dilution
Lane 1 : Lysate from HEK293 cells
Lane 2 : Lysate from HEK293 cells transfected with CD14 (amino acids 20-214)-hIgGFc
Predicted band size: 40 kDa -
All lanes : Anti-CD14 antibody [4B4F12] (ab182032) at 1/500 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : A549 cell lysate
Lane 3 : HL60 cell lysate
Lane 4 : mouse RAW264.7 cell lysate
Lane 5 : HeLa cell lysate
Lane 6 : HEK293 cell lysate
Lane 7 : mouse NIH 3T3 cell lysate
Predicted band size: 40 kDa
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (28)
ab182032 は 28 報の論文で使用されています。
- Guo D et al. ELK1-Induced upregulation of long non-coding TNK2-AS1 promotes the progression of acute myeloid leukemia by EZH2-mediated epigenetic silencing of CELF2. Cell Cycle 22:117-130 (2023). PubMed: 35941836
- Mo YQ et al. Lysosomal exocytosis of HSP70 stimulates monocytic BMP6 expression in Sjögren's syndrome. J Clin Invest 132:N/A (2022). PubMed: 35113815
- Nakamura A et al. The potential of soluble CD14 in discriminating nonalcoholic steatohepatitis from nonalcoholic fatty liver disease. Hepatol Res 52:508-521 (2022). PubMed: 35129841
- Wu Z et al. sGRP78 enhances selective autophagy of monomeric TLR4 to regulate myeloid cell death. Cell Death Dis 13:587 (2022). PubMed: 35798718
- Zadka Ł et al. Interplay of stromal tumor-infiltrating lymphocytes, normal colonic mucosa, cancer-associated fibroblasts, clinicopathological data and the immunoregulatory molecules of patients diagnosed with colorectal cancer. Cancer Immunol Immunother 70:2681-2700 (2021). PubMed: 33625532