リコンビナントRabMAb

Anti-CD137 抗体 [EPR23218-111] - BSA and Azide free (ab270527)

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Immunocytochemistry/ Immunofluorescence - Anti-CD137 antibody [EPR23218-111] - BSA and Azide free (ab270527)
  • Flow Cytometry - Anti-CD137 antibody [EPR23218-111] - BSA and Azide free (ab270527)
  • Immunoprecipitation - Anti-CD137 antibody [EPR23218-111] - BSA and Azide free (ab270527)
  • Immunocytochemistry/ Immunofluorescence - Anti-CD137 antibody [EPR23218-111] - BSA and Azide free (ab270527)
  • Anti-CD137 antibody [EPR23218-111] - BSA and Azide free (ab270527)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23218-111] to CD137 - BSA and Azide free
  • Suitable for: WB, ICC/IF, IP, Flow Cyt
  • Reacts with: Mouse

製品の概要

  • 製品名

    Anti-CD137 antibody [EPR23218-111] - BSA and Azide free
    CD137 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR23218-111] to CD137 - BSA and Azide free

  • 由来種

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Mouse
    ICC/IF
    Human
    IP
    Mouse
    See all applications and species data

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: His-tagged mouse CD137 recombinant protein(aa24-187); mouse splenocyte lysate (+/- Con A treatment). ICC/IF: Mouse splenocytes treated with Con A; HEK-293T cells transfected with GFP-tagged CD137 expression vector. Flow cyt: Mouse splenocytes treatedwith Con A. IP: Mouse splenocyte whole cell lysate (Con A treated).

  • 特記事項

    ab270527 is the carrier-free version of ab243648. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab270527 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

アプリケーション Species
Flow Cyt
Mouse
ICC/IF
Human
IP
Mouse
アプリケーション Abreviews 特記事項
WB
Use at an assay dependent concentration. Predicted molecular weight: 28 kDa.
ICC/IF
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent concentration.
特記事項
WB
Use at an assay dependent concentration. Predicted molecular weight: 28 kDa.
ICC/IF
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent concentration.

ターゲット情報

  • 機能

    Receptor for TNFSF14/4-1BBL. Possibly active during T cell activation.

  • 組織特異性

    Expressed on the surface of activated T-cells.

  • 配列類似性

    Contains 4 TNFR-Cys repeats.

  • 細胞内局在

    Membrane.
  • Information by UniProt

  • 参照データベース

    • 別名

      • 4 1BB antibody
      • 4 1BB ligand receptor antibody
      • 4-1BB ligand receptor antibody
      • 4-1BB Ligand Receptor T Cell antibody
      • 4-1BB, mouse, homolog of antibody
      • Antigen 4-1BB Homolog antibody
      • CD 137 antibody
      • CD137 antibody
      • CD137 antigen antibody
      • CDw137 antibody
      • HLDA VI antibody
      • Homolog of mouse 4 1BB antibody
      • ILA antibody
      • induced by lymphocyte activation (ILA) antibody
      • Induced by lymphocyte activation antibody
      • Interleukin activated receptor homolog of mouse Ly63 antibody
      • Ly63, mouse, homolog of antibody
      • MGC2172 antibody
      • OTTHUMP00000044294 antibody
      • Receptor protein 4 1BB antibody
      • T cell antigen 4 1BB homolog antibody
      • T cell antigen ILA antibody
      • T-cell antigen 4-1BB homolog antibody
      • T-cell antigen ILA antibody
      • TNF receptor superfamily member 9 antibody
      • TNFRSF9 antibody
      • TNR9_HUMAN antibody
      • Tumor necrosis factor receptor superfamily member 9 antibody
      see all

    画像

    • Immunocytochemistry/ Immunofluorescence - Anti-CD137 antibody [EPR23218-111] - BSA and Azide free (ab270527)
      Immunocytochemistry/ Immunofluorescence - Anti-CD137 antibody [EPR23218-111] - BSA and Azide free (ab270527)

      Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HEK-293T cells labelling CD137 with ab243648 at 1/100 dilution, followed by ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) antibody at 1/1000 dilution (Red). Confocal image showing cytoplasmic and membranous staining in HEK-293T cells transfected with a GFP-tagged CD137 expression vector -  the GPF tag is shown in Green. The Nuclear counterstain was DAPI (Blue).

      Secondary antibody only control: Secondary antibody is ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) at 1/1000 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab243648).

    • Flow Cytometry - Anti-CD137 antibody [EPR23218-111] - BSA and Azide free (ab270527)
      Flow Cytometry - Anti-CD137 antibody [EPR23218-111] - BSA and Azide free (ab270527)

      Flow cytometric analysis of Mouse splenocytes (treated with 2.5ug/ml concanavalin A  for 72h) cells labelling CD137 with ab243648 at 1/500 (Right) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Left).

      Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

      Cells were stained with rabbit IgG (Left) or ab243648 (Right), then stained with anti-CD25 conjugated to BV421.

      Gated on viable cells.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab243648).

    • Immunoprecipitation - Anti-CD137 antibody [EPR23218-111] - BSA and Azide free (ab270527)
      Immunoprecipitation - Anti-CD137 antibody [EPR23218-111] - BSA and Azide free (ab270527)

      CD137 was immunoprecipitated from 0.35 mg mouse splenocyte (treated with 2.5 μg/ml Concanavalin A for 72 hours) whole cell lysate with ab243648 at 1/30 dilution. Western blot was performed on the immunoprecipitate using ab243648 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

      Lane 1: Mouse splenocyte (treated with 2.5 μg/ml Concanavalin A for 72 hours) whole cell lysate 10 μg

      Lane 2: ab243648 IP in Mouse splenocyte (treated as above)  whole cell lysate

      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab243648 in Mouse splenocyte (treated as above) whole cell lysate

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

      Exposure time: 76 seconds.

      CD137 is a type I transmembrane glycoprotein and has both monomeric and dimeric forms on the surface of activated T cells (PMID: 30355497).

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab243648).

       

    • Immunocytochemistry/ Immunofluorescence - Anti-CD137 antibody [EPR23218-111] - BSA and Azide free (ab270527)
      Immunocytochemistry/ Immunofluorescence - Anti-CD137 antibody [EPR23218-111] - BSA and Azide free (ab270527)

      Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Mouse splenocytes cells labelling CD137 with ab243648 at 1/100 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic and membranous staining in mouse splenocytes treated with Concanavalin A (2.5 ug/ml) for 72 h. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

      Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab243648).

    • Anti-CD137 antibody [EPR23218-111] - BSA and Azide free (ab270527)
      Anti-CD137 antibody [EPR23218-111] - BSA and Azide free (ab270527)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab270527 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab270527 は論文での使用が確認できていません。

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