Anti-CD137 抗体 [EPR23218-111] (ab243648)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23218-111] to CD137
- Suitable for: ICC/IF, IP, Flow Cyt, WB
- Reacts with: Mouse
Related conjugates and formulations
製品の概要
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製品名
Anti-CD137 antibody [EPR23218-111]
CD137 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR23218-111] to CD137 -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, IP, Flow Cyt, WBmore details
適用なし: IHC-P -
種交差性
交差種: Mouse -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- WB: His-tagged mouse CD137 recombinant protein(aa24-187); mouse splenocyte lysate (+/- Con A treatment). ICC/IF: Mouse splenocytes treated with Con A; HEK-293T cells transfected with GFP-tagged mouse CD137 expression vector. Flow cyt: Mouse splenocytes treatedwith Con A. IP: Mouse splenocyte whole cell lysate (Con A treated).
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR23218-111 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab243648の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
ICC/IF |
1/100.
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IP |
1/30.
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Flow Cyt |
1/500.
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WB |
1/1000. Predicted molecular weight: 28 kDa.
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特記事項 |
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ICC/IF
1/100. |
IP
1/30. |
Flow Cyt
1/500. |
WB
1/1000. Predicted molecular weight: 28 kDa. |
ターゲット情報
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機能
Receptor for TNFSF14/4-1BBL. Possibly active during T cell activation. -
組織特異性
Expressed on the surface of activated T-cells. -
配列類似性
Contains 4 TNFR-Cys repeats. -
細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 21942 Mouse
- SwissProt: P20334 Mouse
- Unigene: 244187 Mouse
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別名
- 4 1BB antibody
- 4 1BB ligand receptor antibody
- 4-1BB ligand receptor antibody
see all
画像
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Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HEK-293T cells labelling CD137 with ab243648 at 1/100 dilution, followed by ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) antibody at 1/1000 dilution (Red). Confocal image showing cytoplasmic and membranous staining in HEK-293T cells transfected with a GFP-tagged CD137 expression vector - the GPF tag is shown in Green. The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) at 1/1000 dilution.
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All lanes : Anti-CD137 antibody [EPR23218-111] (ab243648) at 1/1000 dilution
Lane 1 : Untreated mouse splenocytes, whole cell lysate
Lane 2 : Mouse splenocytes (treated with 2.5 µg/ml Concanavalin A for 72 hours), whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
Predicted band size: 28 kDa
Observed band size: 21-24,36-40 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST.
CD137 is a type I transmembrane glycoprotein and has both monomeric and dimeric forms on the surface of activated T cells (PMID: 30355497).
Exposure time: 3 minutes.
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Flow cytometric analysis of Mouse splenocytes (treated with 2.5ug/ml concanavalin A for 72h) cells labelling CD137 with ab243648 at 1/500 (Right) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Left).
Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.
Cells were stained with rabbit IgG (Left) or ab243648 (Right), then stained with anti-CD25 conjugated to BV421.
Gated on viable cells.
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All lanes : Anti-CD137 antibody [EPR23218-111] (ab243648) at 1/1000 dilution
Lane 1 : His-tagged mouse CD137 recombinant protein(aa24-187), 10 ng
Lane 2 : His-tagged human CD137 recombinant protein(aa24-186), 10 ng
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 28 kDaBlocking and diluting buffer and concentration: 5% NFDM/TBST.
Exposure time: 15 seconds.
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Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Mouse splenocytes cells labelling CD137 with ab243648 at 1/100 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic and membranous staining in mouse splenocytes treated with Concanavalin A (2.5 ug/ml) for 72 h. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.
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CD137 was immunoprecipitated from 0.35 mg mouse splenocyte (treated with 2.5 μg/ml Concanavalin A for 72 hours) whole cell lysate with ab243648 at 1/30 dilution. Western blot was performed on the immunoprecipitate using ab243648 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: Mouse splenocyte (treated with 2.5 μg/ml Concanavalin A for 72 hours) whole cell lysate 10 μg
Lane 2: ab243648 IP in Mouse splenocyte (treated as above) whole cell lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab243648 in Mouse splenocyte (treated as above) whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 76 seconds.
CD137 is a type I transmembrane glycoprotein and has both monomeric and dimeric forms on the surface of activated T cells (PMID: 30355497).
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
Certificate of Compliance
参考文献 (1)
ab243648 は 1 報の論文で使用されています。
- Cao Y et al. LSD1 in beige adipocytes protects cardiomyocytes against oxygen and glucose deprivation. Iran J Basic Med Sci 26:30-36 (2023). PubMed: 36594068