Anti-CD134 / OX40L receptor 抗体 [OX40/3108] (ab270727)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse monoclonal [OX40/3108] to CD134 / OX40L receptor
- Suitable for: Protein Array, IHC-P, ICC/IF, Flow Cyt
- Reacts with: Human
製品の概要
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製品名
Anti-CD134 / OX40L receptor antibody [OX40/3108]
CD134 / OX40L receptor 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [OX40/3108] to CD134 / OX40L receptor -
由来種
Mouse -
Tested Applications & Species
Application Species Flow Cyt HumanICC/IF HumanIHC-P Human -
免疫原
Recombinant fragment within Human CD134/ OX40L receptor aa 59-205. The exact sequence is proprietary.
Database link: P43489 -
ポジティブ・コントロール
- IHC-P: Human renal cell carcinoma and human tonsil tissue. ICC/IF: MOLT4 cells. Flow cyt: MOLT4 cells.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.05% BSA -
Concentration information loading...
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精製度
Protein A/G purified -
特記事項(精製)
Purified from Bioreactor Concentrate. -
ポリ/モノ
モノクローナル -
クローン名
OX40/3108 -
アイソタイプ
IgG2c -
軽鎖の種類
kappa -
研究分野
関連製品
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Compatible Secondaries
アプリケーション
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab270727 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
アプリケーション | Species |
---|---|
Flow Cyt |
Human
|
ICC/IF |
Human
|
IHC-P |
Human
|
All applications |
Chimpanzee
|
アプリケーション | Abreviews | 特記事項 |
---|---|---|
Protein Array |
Use at an assay dependent concentration.
|
|
IHC-P |
Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
|
ICC/IF |
Use a concentration of 1 - 2 µg/ml.
|
|
Flow Cyt |
Use 1-2µg for 106 cells.
|
特記事項 |
---|
Protein Array
Use at an assay dependent concentration. |
IHC-P
Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ICC/IF
Use a concentration of 1 - 2 µg/ml. |
Flow Cyt
Use 1-2µg for 106 cells. |
ターゲット情報
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機能
Receptor for TNFSF4/OX40L/GP34. -
配列類似性
Contains 4 TNFR-Cys repeats. -
細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 7293 Human
- Omim: 600315 Human
- SwissProt: P43489 Human
- Unigene: 129780 Human
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別名
- ACT 35 antibody
- ACT35 antibody
- ACT35 antigen antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD134 / OX40L receptor antibody [OX40/3108] (ab270727)
Formalin-fixed, paraffin-embedded human tonsil tissue stained for CD134/OX40L using ab270727 at 2 µg/ml in immunohistochemical analysis.
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Immunocytochemistry/ Immunofluorescence - Anti-CD134 / OX40L receptor antibody [OX40/3108] (ab270727)
Immunofluorescence analysis of human MOLT4 cells labeling CD134/OX40L (Green) using ab270727 at 2 µg/ml, followed by goat anti-mouse IgG conjugated to CF488. Nuclei are stained with Reddot (Red).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD134 / OX40L receptor antibody [OX40/3108] (ab270727)
Formalin-fixed, paraffin-embedded human renal cell carcinoma tissue stained for CD134/OX40L using ab270727 at 2 µg/ml in immunohistochemical analysis.
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Flow cytometry analysis of human MOLT4 cells labeling CD134/OX40L (Blue) using ab270727 at 2 µg/ml, followed by goat anti-mouse IgG conjugated to CF488. Isotype control (Red).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD134 / OX40L receptor antibody [OX40/3108] (ab270727)
Formalin-fixed, paraffin-embedded human renal cell carcinoma tissue stained for CD134/OX40L using ab270727 at 2 µg/ml in immunohistochemical analysis.
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Analysis of Protein Array containing more than 19,000 full-length human proteins using ab270727.
Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD’s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD’s) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
参考文献 (0)
ab270727 は論文での使用が確認できていません。