Anti-CD130 (gp130) 抗体 - C-terminal (ab227058)
Key features and details
- Rabbit polyclonal to CD130 (gp130) - C-terminal
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-CD130 (gp130) antibody - C-terminal
CD130 (gp130) 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to CD130 (gp130) - C-terminal -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-P, ICC/IFmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Dog, Pig, Rhesus monkey -
免疫原
Recombinant fragment within Human CD130 (gp130) (C terminal). The exact sequence is proprietary.
Database link: P40189 -
ポジティブ・コントロール
- WB: HepG2 whole cell lysate (ab7900). IHC-P: J5 xenograft tissue. ICC/IF: HeLa cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab227058の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/500 - 1/3000. Predicted molecular weight: 37,96,104 kDa.
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IHC-P |
1/100 - 1/1000.
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ICC/IF |
1/100 - 1/1000.
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特記事項 |
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WB
1/500 - 1/3000. Predicted molecular weight: 37,96,104 kDa. |
IHC-P
1/100 - 1/1000. |
ICC/IF
1/100 - 1/1000. |
ターゲット情報
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機能
Signal-transducing molecule. The receptor systems for IL6, LIF, OSM, CNTF, IL11, CTF1 and BSF3 can utilize gp130 for initiating signal transmission. Binds to IL6/IL6R (alpha chain) complex, resulting in the formation of high-affinity IL6 binding sites, and transduces the signal. Does not bind IL6. May have a role in embryonic development (By similarity). The type I OSM receptor is capable of transducing OSM-specific signaling events. -
組織特異性
Found in all the tissues and cell lines examined. Expression not restricted to IL6 responsive cells. -
配列類似性
Belongs to the type I cytokine receptor family. Type 2 subfamily.
Contains 5 fibronectin type-III domains.
Contains 1 Ig-like C2-type (immunoglobulin-like) domain. -
ドメイン
The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
The box 1 motif is required for JAK interaction and/or activation. -
翻訳後修飾
Phosphorylation of Ser-782 down-regulates cell surface expression.
Heavily N-glycosylated. -
細胞内局在
Secreted and Cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 3572 Human
- Entrez Gene: 16195 Mouse
- Entrez Gene: 25205 Rat
- Entrez Gene: 574127 Rhesus monkey
- Omim: 600694 Human
- SwissProt: P40189 Human
- SwissProt: Q00560 Mouse
- SwissProt: P40190 Rat
see all -
別名
- CD130 antibody
- CD130 antigen antibody
- CDw130 antibody
see all
画像
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Anti-CD130 (gp130) antibody - C-terminal (ab227058) at 1/1000 dilution + HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate at 30 µg
Secondary
HRP-conjugated anti-rabbit IgG
Predicted band size: 37,96,104 kDa7.5% SDS-PAGE gel.
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HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling CD130 (gp130) (green) using ab227058 in ICC/IF. Cells were fixed in ice-cold methanol for 5 minutes. Nuclear counterstain: Hoechst 33342.
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Paraffin-embedded human J5 xenograft tissue stained for CD130 (gp130) using ab227058 at 1/500 dilution in immunohistochemical analysis.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab227058 は論文での使用が確認できていません。