Anti-CD13 抗体 [SP187] (ab227663)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [SP187] to CD13
- Suitable for: ICC/IF, WB, IHC-P
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-CD13 antibody [SP187]
CD13 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [SP187] to CD13 -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, WB, IHC-Pmore details
適用なし: Flow Cyt -
種交差性
交差種: Human
交差が予測される動物種: Rabbit -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- WB: THP-1, PANC-1, HEK293 and U937 cell lysate. IHC-P: Human kidney, tonsil, spleen, thymus, lung, liver, placenta, bladder, bone marrow, reactive lymph node, HK lymphoma and B-cell lymphoma tissues. ICC/IF: THP-1 cells
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading...
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精製度
Protein A/G purified -
特記事項(精製)
Purified from TCS by protein A/G. -
ポリ/モノ
モノクローナル -
クローン名
SP187 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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KO cell lines
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KO cell lysates
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab227663の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC/IF |
1/25.
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WB |
1/400. Predicted molecular weight: 109 kDa.
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IHC-P |
1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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特記事項 |
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ICC/IF
1/25. |
WB
1/400. Predicted molecular weight: 109 kDa. |
IHC-P
1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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機能
Broad specificity aminopeptidase. Plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases. May play a critical role in the pathogenesis of cholesterol gallstone disease. May be involved in the metabolism of regulatory peptides of diverse cell types including small intestinal and tubular epithelial cells, macrophages, granulocytes and synaptic membranes from the CNS. Found to cleave antigen peptides bound to major histocompatibility complex class II molecules of presenting cells and to degrade neurotransmitters at synaptic junctions. Is also implicated as a regulator of IL-8 bioavailability in the endometrium, and therefore may contribute to the regulation of angiogenesis. Is used as a marker for acute myeloid leukemia and plays a role in tumor invasion. In case of human coronavirus 229E (HCoV-229E) infection, serves as receptor for HCoV-229E spike glycoprotein. Mediates as well human cytomegalovirus (HCMV) infection. -
組織特異性
Expressed in epithelial cells of the kidney, intestine, and respiratory tract; granulocytes, monocytes, fibroblasts, endothelial cells, cerebral pericytes at the blood-brain barrier, synaptic membranes of cells in the CNS. Also expressed in endometrial stromal cells, but not in the endometrial glandular cells. Found in the vasculature of tissues that undergo angiogenesis and in malignant gliomas and lymph node metastases from multiple tumor types but not in blood vessels of normal tissues. A soluble form has been found in plasma. It is found to be elevated in plasma and effusions of cancer patients. -
配列類似性
Belongs to the peptidase M1 family. -
ドメイン
Amino acids 260-353 are essential to mediate susceptibility to infection with HCoV-229E (in porcine/human chimeric studies) and more specifically amino acids 288-295 (mutagenesis studies). -
翻訳後修飾
Sulfated.
N- and O-glycosylated.
May undergo proteolysis and give rise to a soluble form. -
細胞内局在
Cell membrane. Cytoplasm > cytosol. A soluble form has also been detected. - Information by UniProt
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参照データベース
- Entrez Gene: 290 Human
- Omim: 151530 Human
- SwissProt: P15144 Human
- Unigene: 1239 Human
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別名
- Alanyl (membrane) aminopeptidase antibody
- Alanyl aminopeptidase antibody
- Aminopeptidase M antibody
see all
画像
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ab227663 staining CD13 in wild-type THP-1 cells (top panel) and ANPEP knockout THP-1 cells (bottom panel) (ab273759). The cells were fixed with 4% paraformaldehyde (10 min) then permeabilized with 0.1% Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab227663 at 1μg/ml concentration and ab7291 (Mouse monoclonal to alpha Tubulin) at 1/1000 dilution overnight at 4°C followed by a further incubation at room temperature for 1h with a goat secondary antibody to rabbit IgG (Alexa Fluor® 488) (ab150081) at 2 μg/ml (shown in green) and a goat secondary antibody to mouse IgG (Alexa Fluor® 594) (ab150120) at 2 μg/ml (shown in red). Nuclear DNA was labelled in blue with DAPI.
Image was taken with a confocal microscope (Leica-Microsystems TCS SP8). -
Formalin-fixed, paraffin-embedded human kidney tissue stained for CD13 using ab227663 at 1/100 dilution in immunohistochemical analysis.
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All lanes : Anti-CD13 antibody [SP187] (ab227663) at 1/400 dilution
Lane 1 : Wild-type THP-1 cell lysate
Lane 2 : ANPEP knockout THP-1 cell lysate
Lane 3 : PANC-1 cell lysate
Lane 4 : HEK-293 cell lysate
Lysates/proteins at 30 µg per lane.
Performed under reducing conditions.
Predicted band size: 109 kDa
Observed band size: 160 kDa why is the actual band size different from the predicted?Lanes 1 - 4: Merged signal (red and green). Green - ab227663 observed at 160 kDa. Red - loading control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.
ab227663 was shown to react with CD13 in wild-type THP-1 cells in western blot with loss of signal observed in ANPEP knockout cell line ab273759 (knockout cell lysate ab275505). Wild-type and ANPEP knockout THP-1 cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with ab227663 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 400 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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Immunocytochemistry/ Immunofluorescence analysis of THP-1 (human monocytic leukemia monocyte) cells labeling CD13 with purified ab227663 at 1/25 (5.32µg/ml). Cells were fixed in 100% Methanol. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
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Anti-CD13 antibody [SP187] (ab227663) at 1/400 dilution + U937 (human histiocytic lymphoma cell line) cell lysate
Predicted band size: 109 kDa -
Formalin-fixed, paraffin-embedded human tonsil tissue stained for CD13 using ab227663 at 1/100 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human spleen tissue stained for CD13 using ab227663 at 1/100 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human reactive lymph node tissue stained for CD13 using ab227663 at 1/100 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human thymus tissue stained for CD13 using ab227663 at 1/100 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human lung tissue stained for CD13 using ab227663 at 1/100 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human liver tissue stained for CD13 using ab227663 at 1/100 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human placenta tissue stained for CD13 using ab227663 at 1/100 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human bladder tissue stained for CD13 using ab227663 at 1/100 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human HK lymphoma tissue stained for CD13 using ab227663 at 1/100 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human B-cell lymphoma tissue stained for CD13 using ab227663 at 1/100 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human bone marrow tissue stained for CD13 using ab227663 at 1/100 dilution in immunohistochemical analysis.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab227663 は 1 報の論文で使用されています。
- Wei X et al. MicroRNA-1305 Inhibits the Stemness of LCSCs and Tumorigenesis by Repressing the UBE2T-Dependent Akt-Signaling Pathway. Mol Ther Nucleic Acids 16:721-732 (2019). PubMed: 31128423