Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Brain)
Permeabilization
No
Specification
Brain
Blocking step
Serum as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: 23°C
Fixative
Methanol
Other product details
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% normal goat serum in PBS
Dilution
1/750
Secondary antibody
Dilution
1/500
Name
Non-Abcam antibody was used: ThermoFisher
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Additional data
Additional Notes
Both methanol and acetone fixation for 5 minutes at -20C worked.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MR. Victor Bernal
Verified customer
投稿 Jul 23 2018