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Immunology Cell Type Markers CD Myeloid Cells
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遺伝子ノックアウト細胞株 検証済リコンビナントRabMAb

Anti-CD13 抗体 [EPR4058] (ab108310)

  • Datasheet
Reviews (10)Q&A (2)References (8)

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Abpromise

保証された製品品質、優れたカスタマー・サポート。 詳細はこちら。

Immunocytochemistry - Anti-CD13 antibody [EPR4058] (ab108310)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD13 antibody [EPR4058] (ab108310)
  • Western blot - Anti-CD13 antibody [EPR4058] (ab108310)
  • Immunocytochemistry - Anti-CD13 antibody [EPR4058] (ab108310)
  • Western blot - Anti-CD13 antibody [EPR4058] (ab108310)
  • Western blot - Anti-CD13 antibody [EPR4058] (ab108310)
  • Western blot - Anti-CD13 antibody [EPR4058] (ab108310)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD13 antibody [EPR4058] (ab108310)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD13 antibody [EPR4058] (ab108310)
  • Western blot - Anti-CD13 antibody [EPR4058] (ab108310)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD13 antibody [EPR4058] (ab108310)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD13 antibody [EPR4058] (ab108310)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD13 antibody [EPR4058] (ab108310)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD13 antibody [EPR4058] (ab108310)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD13 antibody [EPR4058] (ab108310)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD13 antibody [EPR4058] (ab108310)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD13 antibody [EPR4058] (ab108310)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD13 antibody [EPR4058] (ab108310)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD13 antibody [EPR4058] (ab108310)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD13 antibody [EPR4058] (ab108310)
  • Anti-CD13 antibody [EPR4058] (ab108310)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4058] to CD13
  • Suitable for: ICC, WB, IHC-P
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

こちらの製品もご検討ください

タンパク質
Product image
Recombinant human CD13 protein (ab134454)
一次抗体
Product image
HRP Anti-Interferon gamma antibody [EPR1108] (ab194149)
二次抗体
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

関連製品

製品の概要

  • 製品名

    Anti-CD13 antibody [EPR4058]
    CD13 一次抗体 製品一覧
  • 製品の詳細

    Rabbit monoclonal [EPR4058] to CD13
  • 由来種

    Rabbit
  • アプリケーション

    適用あり: ICC, WB, IHC-Pmore details
    適用なし: Flow Cyt or IP
  • 種交差性

    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide within Human CD13 aa 400-500. The exact sequence is proprietary.

  • ポジティブ・コントロール

    • WB: THP-1, HAP1, PANC-1 and HeLa cell lysate. Mouse and rat kidney lysates; IHC-P: Human kidney, liver, hepatocellular carcinoma, prostatic carcinoma, astrocytoma and breast tissues; mouse and rat kidney tissues; ICC: THP-1 cells.
  • 特記事項

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • バッファー

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • 精製度

    Protein A purified
  • ポリ/モノ

    モノクローナル
  • クローン名

    EPR4058
  • アイソタイプ

    IgG
  • 研究分野

    • Immunology
    • Cell Type Markers
    • CD
    • Myeloid Cells
    • Stem Cells
    • Mesenchymal Stem Cells
    • Surface Molecules
    • Stem Cells
    • Hematopoietic Progenitors
    • Lymphoid
    • B Lymphocytic Lineage
    • Stem Cells
    • Hematopoietic Progenitors
    • Myeloid
    • Dendritic Cell Lineage
    • Stem Cells
    • Hematopoietic Progenitors
    • Myeloid
    • Monocytic Lineage

関連製品

  • Alternative Versions

    • Biotin Anti-CD13 antibody [EPR4058] (ab197630)
    • Anti-CD13 antibody [EPR4058] - Low endotoxin, Azide free (ab227111)
    • Anti-CD13 antibody [EPR4058] - BSA and Azide free (ab271872)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773)
    • Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • KO cell lines

    • Human ANPEP (CD13) knockout THP-1 cell line (ab273759)
  • KO cell lysates

    • Human ANPEP (CD13) knockout THP-1 cell lysate (ab275505)
  • Positive Controls

    • THP1 whole cell lysate (ab7913)
  • Recombinant Protein

    • Recombinant human CD13 protein (ab134454)

アプリケーション

Our Abpromise guarantee covers the use of ab108310 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC 1/100.
WB 1/1000 - 1/10000. Predicted molecular weight: 110 kDa.
IHC-P 1/1600. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

For unpurified use at 1/250 - 1/500.

  • 追加情報
    Is unsuitable for Flow Cyt or IP.
  • ターゲット情報

    • 機能

      Broad specificity aminopeptidase. Plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases. May play a critical role in the pathogenesis of cholesterol gallstone disease. May be involved in the metabolism of regulatory peptides of diverse cell types including small intestinal and tubular epithelial cells, macrophages, granulocytes and synaptic membranes from the CNS. Found to cleave antigen peptides bound to major histocompatibility complex class II molecules of presenting cells and to degrade neurotransmitters at synaptic junctions. Is also implicated as a regulator of IL-8 bioavailability in the endometrium, and therefore may contribute to the regulation of angiogenesis. Is used as a marker for acute myeloid leukemia and plays a role in tumor invasion. In case of human coronavirus 229E (HCoV-229E) infection, serves as receptor for HCoV-229E spike glycoprotein. Mediates as well human cytomegalovirus (HCMV) infection.
    • 組織特異性

      Expressed in epithelial cells of the kidney, intestine, and respiratory tract; granulocytes, monocytes, fibroblasts, endothelial cells, cerebral pericytes at the blood-brain barrier, synaptic membranes of cells in the CNS. Also expressed in endometrial stromal cells, but not in the endometrial glandular cells. Found in the vasculature of tissues that undergo angiogenesis and in malignant gliomas and lymph node metastases from multiple tumor types but not in blood vessels of normal tissues. A soluble form has been found in plasma. It is found to be elevated in plasma and effusions of cancer patients.
    • 配列類似性

      Belongs to the peptidase M1 family.
    • ドメイン

      Amino acids 260-353 are essential to mediate susceptibility to infection with HCoV-229E (in porcine/human chimeric studies) and more specifically amino acids 288-295 (mutagenesis studies).
    • 翻訳後修飾

      Sulfated.
      N- and O-glycosylated.
      May undergo proteolysis and give rise to a soluble form.
    • 細胞内局在

      Cell membrane. Cytoplasm > cytosol. A soluble form has also been detected.
    • Target information above from: UniProt accession P15144 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • 参照データベース

      • Entrez Gene: 290 Human
      • Entrez Gene: 16790 Mouse
      • Entrez Gene: 81641 Rat
      • Omim: 151530 Human
      • SwissProt: P15144 Human
      • SwissProt: P97449 Mouse
      • SwissProt: P15684 Rat
      • Unigene: 1239 Human
      • Unigene: 4487 Mouse
      • Unigene: 11132 Rat
      • Unigene: 179371 Rat
      see all
    • 別名

      • Alanyl (membrane) aminopeptidase antibody
      • Alanyl aminopeptidase antibody
      • Aminopeptidase M antibody
      • Aminopeptidase N antibody
      • AMPN_HUMAN antibody
      • ANPEP antibody
      • AP M antibody
      • AP N antibody
      • AP-M antibody
      • AP-N antibody
      • APN antibody
      • CD 13 antibody
      • CD13 antibody
      • CD13 antigen antibody
      • gp150 antibody
      • hAPN antibody
      • LAP 1 antibody
      • LAP1 antibody
      • Microsomal aminopeptidase antibody
      • Myeloid plasma membrane glycoprotein CD13 antibody
      • p150 antibody
      • PEPN antibody
      see all

    画像

    • Immunocytochemistry - Anti-CD13 antibody [EPR4058] (ab108310)
      Immunocytochemistry - Anti-CD13 antibody [EPR4058] (ab108310)
      ab108310 staining CD13 in wild-type THP-1 cells (top panel) and ANPEP knockout THP-1 cells (bottom panel) (ab273759). The cells were fixed with 4% paraformaldehyde (10 min) then permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab108310 at 1/500 dilution and ab7291 (Mouse monoclonal to alpha Tubulin) at 1/1000 dilution overnight at 4°C followed by a further incubation at room temperature for 1h with a goat secondary antibody to rabbit IgG (Alexa Fluor® 488) (ab150081) at 2 μg/ml (shown in green) and a goat secondary antibody to mouse IgG (Alexa Fluor® 594) (ab150120) at 2 μg/ml (shown in red). Nuclear DNA was labelled in blue with DAPI.
      Image was taken with a confocal microscope (Leica-Microsystems TCS SP8).
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD13 antibody [EPR4058] (ab108310)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD13 antibody [EPR4058] (ab108310)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue sections labeling CD13 with purified ab108310 at 1/1600 dilution (0.43 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

    • Western blot - Anti-CD13 antibody [EPR4058] (ab108310)
      Western blot - Anti-CD13 antibody [EPR4058] (ab108310)
      All lanes : Anti-CD13 antibody [EPR4058] (ab108310) at 1/1000 dilution

      Lane 1 : Wild-type THP-1 cell lysate
      Lane 2 : ANPEP knockout THP-1 cell lysate
      Lane 3 : PANC-1 cell lysate
      Lane 4 : HEK-293 cell lysate

      Lysates/proteins at 30 µg per lane.

      Performed under reducing conditions.

      Predicted band size: 110 kDa
      Observed band size: 160 kDa
      why is the actual band size different from the predicted?



      Lanes 1 - 4: Merged signal (red and green). Green - ab108310 observed at 160 kDa. Red - loading control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.

      ab108310 was shown to react with CD13 in wild-type THP-1 cells in western blot with loss of signal observed in ANPEP knockout cell line ab273759 (knockout cell lysate ab275505). Wild-type and ANPEP knockout THP-1 cell lysates were subjected to SDS-PAGE. Membranes were blocked in fluorescent western blot (TBS-based) blocking solution before incubation with ab108310 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

    • Immunocytochemistry - Anti-CD13 antibody [EPR4058] (ab108310)
      Immunocytochemistry - Anti-CD13 antibody [EPR4058] (ab108310)

      Confocal image showing membranous staining in THP-1 cells

      ab108310 (purified) at 1/100 staining CD13 in the THP-1 (human monocytic leukemia monocyte) cell line by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 100% methanol. Samples were incubated with primary antibody 1/500. ab150077 An Alexa Fluor® 488-conjugated Goat anti-Rabbit IgG at 1/1000 was used as the secondary antibody. Ab195889  Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 was used as a counter stain and DAPI was used as a nuclear counter stain. 

    • Western blot - Anti-CD13 antibody [EPR4058] (ab108310)
      Western blot - Anti-CD13 antibody [EPR4058] (ab108310)

      Lane 1: Wild type HAP1 whole cell lysate (20 µg)
      Lane 2: Empty
      Lane 3: CD13 knockout HAP1 whole cell lysate (20 µg)
      Lane 4: Empty
      Lane 5: HeLa whole cell lysate (20 µg)

      Lanes 1 - 5: Merged signal (red and green). Green - ab108310 (unpurified) observed at 160 kDa. Red - loading control, ab18058, observed at 130 kDa.

      ab108310 was shown to recognize CD13 when CD13 knockout samples were used, along with additional cross-reactive bands. Wild-type and CD13 knockout samples were subjected to SDS-PAGE.  Ab108310 and ab18058 (Mouse anti Vinculin loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

    • Western blot - Anti-CD13 antibody [EPR4058] (ab108310)
      Western blot - Anti-CD13 antibody [EPR4058] (ab108310)
      Anti-CD13 antibody [EPR4058] (ab108310) at 1/20000 dilution (Purified) + Mouse kidney lysates at 15 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 110 kDa
      Observed band size: 150 kDa why is the actual band size different from the predicted?

    • Western blot - Anti-CD13 antibody [EPR4058] (ab108310)
      Western blot - Anti-CD13 antibody [EPR4058] (ab108310)
      All lanes : Anti-CD13 antibody [EPR4058] (ab108310) at 1/5000 dilution (Purified)

      Lane 1 : THP-1 (Human monocytic leukemia monocyte) whole cell lysates
      Lane 2 : Rat kidney lysates

      Lysates/proteins at 15 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 110 kDa
      Observed band size: 150 kDa why is the actual band size different from the predicted?

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD13 antibody [EPR4058] (ab108310)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD13 antibody [EPR4058] (ab108310)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat kidney tissue sections labeling CD13 with purified ab108310 at 1/1600 dilution (0.43 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD13 antibody [EPR4058] (ab108310)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD13 antibody [EPR4058] (ab108310)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse kidney tissue sections labeling CD13 with purified ab108310 at 1/1600 dilution (0.43 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

    • Western blot - Anti-CD13 antibody [EPR4058] (ab108310)
      Western blot - Anti-CD13 antibody [EPR4058] (ab108310)
      All lanes : Anti-CD13 antibody [EPR4058] (ab108310) at 1/1000 dilution (unpurified)

      Lane 1 : THP-1 cell lysate at 10 µg
      Lane 2 : human fetal liver lysate

      Performed under reducing conditions.

      Predicted band size: 110 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD13 antibody [EPR4058] (ab108310)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD13 antibody [EPR4058] (ab108310)

      ab108310 (unpurified), at 1/250, staining CD13 in human kidney tissue by immunohistochemistry. Heat mediated antigen retrieval was performed using citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD13 antibody [EPR4058] (ab108310)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD13 antibody [EPR4058] (ab108310)

      ab108310 (unpurified) showing positive staining in human normal liver tissue. Heat mediated antigen retrieval was performed using citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD13 antibody [EPR4058] (ab108310)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD13 antibody [EPR4058] (ab108310)

      ab108310 (unpurified) showing positive staining in human hepatocellular carcinoma tissue. Heat mediated antigen retrieval was performed using citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD13 antibody [EPR4058] (ab108310)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD13 antibody [EPR4058] (ab108310)

      ab108310 (unpurified) showing positive staining in human astrocytoma tissue. Heat mediated antigen retrieval was performed using citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD13 antibody [EPR4058] (ab108310)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD13 antibody [EPR4058] (ab108310)

      ab108310 (unpurified) showing positive staining in human normal breast tissue. Heat mediated antigen retrieval was performed using citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD13 antibody [EPR4058] (ab108310)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD13 antibody [EPR4058] (ab108310)

      ab108310 (unpurified) showing positive staining in human prostatic carcinoma tissue. Heat mediated antigen retrieval was performed using citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD13 antibody [EPR4058] (ab108310)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD13 antibody [EPR4058] (ab108310)

      ab108310 (unpurified) showing positive staining in human normal tonsil tissue. Heat mediated antigen retrieval was performed using citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD13 antibody [EPR4058] (ab108310)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD13 antibody [EPR4058] (ab108310)

      ab108310 (unpurified) showing positive staining in human normal stomach tissue. Heat mediated antigen retrieval was performed using citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD13 antibody [EPR4058] (ab108310)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD13 antibody [EPR4058] (ab108310)

      ab108310 (unpurified) showing positive staining in human normal colon tissue. Heat mediated antigen retrieval was performed using citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD13 antibody [EPR4058] (ab108310)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD13 antibody [EPR4058] (ab108310)

      ab108310 (unpurified) showing positive staining in human lung adenocarcinoma tissue. Heat mediated antigen retrieval was performed using citrate buffer pH 6 before commencing with IHC staining protocol.

    • Anti-CD13 antibody [EPR4058] (ab108310)
      Anti-CD13 antibody [EPR4058] (ab108310)

    プロトコール

    • Immunoprecipitation protocols
    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    データシートおよび資料

    • Datasheet
  • 参考文献 (8)

    ab108310 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab108310 は 8 報の論文で使用されています。

    • Balhorn R  et al. Aldosterone Induces DNA Damage and Activation of Nrf2 Mainly in Tubuli of Mouse Kidneys. Int J Mol Sci 21:N/A (2020). PubMed: 32630085
    • Xu K  et al. CD163 and pAPN double-knockout pigs are resistant to PRRSV and TGEV and exhibit decreased susceptibility to PDCoV while maintaining normal production performance. Elife 9:N/A (2020). PubMed: 32876563
    • Shui LJ  et al. Aminopeptidase N expression in the endometrium could affect endometrial receptivity. Biochem Biophys Res Commun 514:469-474 (2019). PubMed: 31056265
    • Harada K  et al. Induction of artificial cancer stem cells from tongue cancer cells by defined reprogramming factors. BMC Cancer 16:548 (2016). PubMed: 27464948
    • Nefla M  et al. The pro-inflammatory cytokine 14-3-3e is a ligand of CD13 in cartilage. J Cell Sci 128:3250-62 (2015). IHC . PubMed: 26208633
    • Vaswani K  et al. The rat placental renin-angiotensin system - a gestational gene expression study. Reprod Biol Endocrinol 13:89 (2015). WB, IHC . PubMed: 26260700
    • Cui SX  et al. 13F-1, a novel 5-fluorouracil prodrug containing an Asn-Gly-Arg (NO2) COOCH3 tripeptide, inhibits human colonic carcinoma growth by targeting Aminopeptidase N (APN/CD13). Eur J Pharmacol 734:50-9 (2014). PubMed: 24726845
    • Härdtner C  et al. High glucose activates the alternative ACE2/Ang-(1-7)/Mas and APN/Ang IV/IRAP RAS axes in pancreatic ß-cells. Int J Mol Med 32:795-804 (2013). WB ; Rat . PubMed: 23942780

    レビューと Q&A

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    レビューを送る 質問を送る

    1-10 of 12 Abreviews or Q&A

    Immunocytochemistry/ Immunofluorescence abreview for Anti-CD13 antibody [EPR4058]

    Poor
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HeLa)
    Permeabilization
    Yes - 0.5% TritonX100
    Specification
    HeLa
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 20% · Temperature: 23°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    投稿 Nov 02 2020

    Immunocytochemistry/ Immunofluorescence abreview for Anti-CD13 antibody [EPR4058]

    Inconclusive
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Rat Cell (INS-1)
    Permeabilization
    Yes - 0.5% TritonX100
    Specification
    INS-1
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 20% · Temperature: 23°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    投稿 Nov 02 2020

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD13 antibody [EPR4058]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Liver)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: EDTA pH 9.0
    Permeabilization
    No
    Specification
    Liver
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: RT°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    投稿 Sep 30 2020

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD13 antibody [EPR4058]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (palatine tonsil)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Tris/EDTA pH 9.0
    Permeabilization
    No
    Specification
    palatine tonsil
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    投稿 Aug 03 2020

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD13 antibody [EPR4058]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (kidney)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Tris/EDTA pH 9.0
    Permeabilization
    No
    Specification
    kidney
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    投稿 Aug 03 2020

    Immunocytochemistry abreview for Anti-CD13 antibody [EPR4058]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry
    Sample
    Pig Cultured Cells (Vero cells)
    Permeabilization
    No
    Specification
    Vero cells
    Fixative
    Acetone
    Read More

    Mahesh Bhandari

    Verified customer

    投稿 Nov 29 2017

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD13 antibody [EPR4058]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Sheep Tissue sections (trochlea bone)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: citrate
    Permeabilization
    No
    Specification
    trochlea bone
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 0.24% · Temperature: RT°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    投稿 Jun 06 2017

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD13 antibody [EPR4058]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Rhesus monkey Tissue sections (Jejunum)
    Antigen retrieval step
    None
    Permeabilization
    No
    Specification
    Jejunum
    Fixative
    Paraformaldehyde
    Read More

    Dr. Li Li

    Verified customer

    投稿 May 31 2017

    Immunocytochemistry/ Immunofluorescence abreview for Anti-CD13 antibody [EPR4058]

    Inconclusive
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Pig Cell (brain pericytes)
    Specification
    brain pericytes
    Blocking step
    Serum as blocking agent for 45 minute(s) · Concentration: 10% · Temperature: 22°C
    Fixative
    Paraformaldehyde
    Read More

    Mrs. Verena Ledwig

    Verified customer

    投稿 Mar 09 2016

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD13 antibody [EPR4058]

    Good
    Abreviews
    Abreviews
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Normal kidney)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate pH6
    Permeabilization
    No
    Specification
    Normal kidney
    Blocking step
    BSA as blocking agent for 15 minute(s) · Concentration: 3%
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    投稿 Feb 24 2016

    1-10 of 12 Abreviews or Q&A

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