Anti-CD11c 抗体 [EPR1346] (ab133246)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR1346] to CD11c
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-CD11c antibody [EPR1346]
CD11c 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR1346] to CD11c -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details
適用なし: Flow Cyt,ICC/IF,IHC-P or IP -
種交差性
交差種: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- HL-60 cell lysates, TPA stimulated; THP1 cell lysates, TPA stimulated.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR1346 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab133246の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 127 kDa.
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特記事項 |
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WB
1/1000 - 1/10000. Predicted molecular weight: 127 kDa. |
ターゲット情報
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機能
Integrin alpha-X/beta-2 is a receptor for fibrinogen. It recognizes the sequence G-P-R in fibrinogen. It mediates cell-cell interaction during inflammatory responses. It is especially important in monocyte adhesion and chemotaxis. -
組織特異性
Predominantly expressed in monocytes and granulocytes. -
配列類似性
Belongs to the integrin alpha chain family.
Contains 7 FG-GAP repeats.
Contains 1 VWFA domain. -
ドメイン
The integrin I-domain (insert) is a VWFA domain. Integrins with I-domains do not undergo protease cleavage. -
細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 3687 Human
- Omim: 151510 Human
- SwissProt: P20702 Human
- Unigene: 248472 Human
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別名
- 95 alpha chain antibody
- 95 antibody
- CD 11c antibody
see all
画像
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All lanes : Anti-CD11c antibody [EPR1346] (ab133246) at 1/1000 dilution
Lane 1 : HL60 cell lysate, TPA stimulated
Lane 2 : THP1 cell lysate, TPA stimulated
Lysates/proteins at 10 µg per lane.
Predicted band size: 127 kDa -
All lanes : Anti-CD11c antibody [EPR1346] (ab133246) at 1/1000 dilution
Lane 1 : THP1 (Human acute monocytic leukemia cell line) Whole Cell Lysate
Lane 2 : THP1 (Human acute monocytic leukemia cell line) + PMA Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 127 kDa
Observed band size: 150 kDa why is the actual band size different from the predicted?
Exposure time: 12 minutesThis blot was produced using a 10% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab133246 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab133246 は論文での使用が確認できていません。