Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Lymph node)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris-EDTA
Permeabilization
No
Specification
Lymph node
Blocking step
Casein as blocking agent for 15 minute(s) · Concentration: 0.25% · Temperature: RT°C
Fixative
Formaldehyde
Other product details
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 0.25% Casein 1xTBS 0.05% Tween
Secondary antibody
Name
Non-Abcam antibody was used: Immunologic BrightVision Poly-HRP-Anti-Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Aug 03 2015